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Drosophila Melanogaster Lab Report

656 Words3 Pages

The species Drosophila Melanogaster was used to test the effects of evolution when it came to selecting against sepia eyed flies. Drosophila Melanogaster or fruit flies were used because of their short reproduction cycle and simplicity. They reproduce and reach maturity fast. In the wild there are two main eye colors for fruit flies red eyes and sepia eyes or dark eyes. The sepia eyes (r) are known to be recessive while the red eyes (R) are dominant. In this lab fruit flies were analyzed for the sepia gene in F2 and F3 generations. This was considered to be microevolution as the flies genotype and phenotype frequencies changed over generations. Microevolution is a change in allele frequencies in a population over time. (Jane Reece 481) Selecting …show more content…

Two different plastic tubes were filled with media and then water was poured in until all the media turned blue in color. After the media turned blue, 3-4 grains of yeast were placed inside the tubes. Next, the flies had to be anesthetized by using FlyNap that was provided by the instructor. Afterwords, the flies needed to be identified by gender, 10 heterozygous male and 10 heterozygous females needed to be placed in one tube. For the second tube the same amount of flies needed to place inside, 10 heterozygous male and 10 heterozygous females. These tubes were then labeled and then placed into an incubator where they were able to mate. After a week the flies were anesthetized once more and then the F1 generation was removed and all the pupa were placed in tubes. After another week past the F2 flies were anesthetized again and documented based on whether they showed the sepia gene. This time all the flies that had sepia eyes were removed from the tube. Next, 10 red eyed F2 male and female flies were placed in a tube to mate and help form the next generation. After another week pasted by the flies were taken out and the flies were analyzed. Only red eyed flies were used in the new cultures. After the F3 generation was allowed to mature they were counted and the numbers of sepia and red eyed flies were counted and

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Pm3110 Unit 1 Assignment 1

532 Words | 3 Pages

There was no indication of phenotypic differences between male and female mutant flies ,illustrating that the mutation was not sex linked because both mutant sexes possessed the same eye mutation. Male flies in both wild type and mutant flies consisted of the sex combs in the front of the legs, and dark coloration located at the end of the abdomen. Both classes of female flies possessed a tiger- striped abdomen and a tag-like tip at the end of the abdomen. After confirmation of our eye mutation we can assume that our mutation can be due to either genes rosy, garnet, brown, purple, and sepia because these are genes that affect the pigmentation color of the Drosophila melanogaster. Our mutation may be caused by the transportation of the enzyme xanthine dehydrogenase to the rosy gene which causes a deficiency in the pigmentation

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1180 Words | 5 Pages

Introduction: The objective of this lab was to study the trait of aldehyde oxidase (AO) in fruit flies. Aldehyde oxidase is responsible for catalyzing the oxidation of many aldehydes. The aldox gene controls the amount of AO activity in Drosophila melanogaster. In the first part of the lab, an enzyme spot test will be performed on two different vials of Drosophila to exhibit the AO activity of both vial 1A and 1B. A positive test for AO test will present a blue color, while a negative test will present no reaction.

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1130 Words | 5 Pages

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1275 Words | 6 Pages

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708 Words | 3 Pages

Then a F1 cross, 4 females and 6 males was made. During third week, the flies from F1 cross were anesthetized in the bottle for scoring purposes. Then at least 100 flies were scored using a chart in lab manual. One member scored flies and one recorded the flies. The female flies need to be virgins so

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Fruit Fly Lab Report

471 Words | 2 Pages

The expected results of these crosses if the genes assort independently, are that all the offspring from the P generation would have wild-type allele with red eyes, long wings and dark bodies, the flies would be heterozygous for each allele. If the genes were sex-linked and have females in P-generation, the traits in F1 generation in males would be wild-type. In the reciprocal cross, males would exhibit phenotypes of female if it is mutant phenotypes because only female would inherited her X chromosome to the male in new generation. The adults from the P generation were removed before the F1 generation developed from

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Drosophila Melanogaster Lab Report

573 Words | 3 Pages

The purpose of this experiment was to determine if there is natural selection against blind, white-eyed male Drosophila melanogaster, as well as if there is any interplay between selection and drift occurring in populations of different size. This was done by creating and monitoring both small and large populations and placing them in an environment with regular light or complete darkness. It was predicted that natural selection would occur against white-eyed males in the light trials, but would not occur in the dark trials. This selection would occur on the level of male mating success and be seen in a decline in the frequency of Q in the light trials. It was also predicted that drift would be larger in small populations, which would be seen

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794 Words | 4 Pages

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942 Words | 4 Pages

Hypothesis IF white eyes are an autosomal dominant trait AND we cross male flies with autosomal recessive sepia eyes with female flies with white eyes THEN the second generation (F2) offspring will all have white eyes. This hypothesis assumes that both white and sepia eyes are an autosomal gene and that there will be no other genes present that will affect the eye color. The hypothesis predicts that the F1 generation will have only the white eye phenotype, but will be carriers for sepia eyes. With each new generation, the variety of eye color should increase. Graph/Table of Results

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1720 Words | 7 Pages

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1536 Words | 7 Pages

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517 Words | 3 Pages

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800 Words | 4 Pages

The model organism used in the O’Sullivan lab: The O’Sullivan lab has opted to use the fruit fly Drosophila melanogaster in order to model HSP. A model organism in which there is a very well conserved homologue to ARL6IP1, CG101026 (Fowler and O’Sullivan 2016). The fruit fly has a number of advantages associated with it as a model organism as for one Drosophila melanogaster has a short life cycle, a rapid generation time and produce a large number of offspring meaning experiments can be performed quite quickly and fly stocks can be maintained inexpensively and easily. In regards to studying neurodegenerative disease specifically, the fruit fly Drosophila melanogaster possesses a well-established nervous system and although an invertebrate species, possess a ventral nerve cord, which is analogous to the spinal cord with motor neurons that connect from the ventral nerve cord and synapse onto muscles at the neuromuscular junction.

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1185 Words | 5 Pages

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