ipl-logo

Drosophila Melanogaster Lab Report

656 Words3 Pages

The species Drosophila Melanogaster was used to test the effects of evolution when it came to selecting against sepia eyed flies. Drosophila Melanogaster or fruit flies were used because of their short reproduction cycle and simplicity. They reproduce and reach maturity fast. In the wild there are two main eye colors for fruit flies red eyes and sepia eyes or dark eyes. The sepia eyes (r) are known to be recessive while the red eyes (R) are dominant. In this lab fruit flies were analyzed for the sepia gene in F2 and F3 generations. This was considered to be microevolution as the flies genotype and phenotype frequencies changed over generations. Microevolution is a change in allele frequencies in a population over time. (Jane Reece 481) Selecting …show more content…

Two different plastic tubes were filled with media and then water was poured in until all the media turned blue in color. After the media turned blue, 3-4 grains of yeast were placed inside the tubes. Next, the flies had to be anesthetized by using FlyNap that was provided by the instructor. Afterwords, the flies needed to be identified by gender, 10 heterozygous male and 10 heterozygous females needed to be placed in one tube. For the second tube the same amount of flies needed to place inside, 10 heterozygous male and 10 heterozygous females. These tubes were then labeled and then placed into an incubator where they were able to mate. After a week the flies were anesthetized once more and then the F1 generation was removed and all the pupa were placed in tubes. After another week past the F2 flies were anesthetized again and documented based on whether they showed the sepia gene. This time all the flies that had sepia eyes were removed from the tube. Next, 10 red eyed F2 male and female flies were placed in a tube to mate and help form the next generation. After another week pasted by the flies were taken out and the flies were analyzed. Only red eyed flies were used in the new cultures. After the F3 generation was allowed to mature they were counted and the numbers of sepia and red eyed flies were counted and

Show More
Related

Pm3110 Unit 1 Assignment 1

532 Words | 3 Pages

There was no indication of phenotypic differences between male and female mutant flies ,illustrating that the mutation was not sex linked because both mutant sexes possessed the same eye mutation. Male flies in both wild type and mutant flies consisted of the sex combs in the front of the legs, and dark coloration located at the end of the abdomen. Both classes of female flies possessed a tiger- striped abdomen and a tag-like tip at the end of the abdomen. After confirmation of our eye mutation we can assume that our mutation can be due to either genes rosy, garnet, brown, purple, and sepia because these are genes that affect the pigmentation color of the Drosophila melanogaster. Our mutation may be caused by the transportation of the enzyme xanthine dehydrogenase to the rosy gene which causes a deficiency in the pigmentation

Read More

Fruit Fly Lab Report

1180 Words | 5 Pages

Introduction: The objective of this lab was to study the trait of aldehyde oxidase (AO) in fruit flies. Aldehyde oxidase is responsible for catalyzing the oxidation of many aldehydes. The aldox gene controls the amount of AO activity in Drosophila melanogaster. In the first part of the lab, an enzyme spot test will be performed on two different vials of Drosophila to exhibit the AO activity of both vial 1A and 1B. A positive test for AO test will present a blue color, while a negative test will present no reaction.

Read More

Bean Beetle Lab Report

1644 Words | 7 Pages

This graph shows that each ratio of beetles had a different mating time so there was no significant difference in the time of the mating ratio beetles. Discussion In experiment our hypothesis stated that the female beetle would lay more eggs on the northern bean because the northern bean looks and feels like the original beans the beetles came from. The original bean which is the black eye peas is where the beetles grew up on for many generations so they are successful and familiar with supported that the beetles would lay majority of their eggs on the Northern beans because the northern beans are close to what the bean beetles originated from, but as we analyzed the data it also showed that the bean beetled laid most of their beans on the Navy beans as well.

Read More

Milkweed Bug Experiment Lab Report

928 Words | 4 Pages

The survivorship initial sample will be dependent on the amount of milkweed bug eggs that hatched from the 35 eggs that we placed in the containers. The amount that hatched will then be compared to the amount of milkweed bugs at the end of the five week

Read More

C. Elegans Lab Report

1130 Words | 5 Pages

The methods of this experiment are really simple. When we started the experiment we all washed our hands and wore gloves. Each group member did their part to conduct and successful experiment, one group member plugs the Bunsen burner to the gas pipe and turned on the gas, then used flint spark lighter to set the flame on the Bunsen burner. While the second group member is setting the dissecting microscope and making sure the lenses are clean. This member is getting all three Petri dishes ready to examine (first Petri dish contains E. coli, the second dish have the mixture of C. elegans, the third dish is where we are transferring female C. elegans to mate up).

Read More

Pill Bugs Lab Report

1275 Words | 6 Pages

Record this information at the end of every minute for ten minutes, in Table 1. For the second part of the experiment, using the pipette and water, place the water on the filter paper on one side of the behavior tray. Allow the filter paper to absorb the water therefore creating the wet environment. Next, place the Pill Bugs in the behavior tray and observe the amount of

Read More

Fruit Fly Lab Report

947 Words | 4 Pages

We placed three female bean beetles and two male bean beetles in each petri dish. We had a total of three petri dishes, one for each trial. The petri dishes were then placed in constant light under the same conditions for a week. After one week, the data was then collected by counting the number of eggs on each bean by using a light microscope and this number was then recorded and compared to each bean

Read More

Fruit Fly Lab Report

708 Words | 3 Pages

Then a F1 cross, 4 females and 6 males was made. During third week, the flies from F1 cross were anesthetized in the bottle for scoring purposes. Then at least 100 flies were scored using a chart in lab manual. One member scored flies and one recorded the flies. The female flies need to be virgins so

Read More

Fruit Fly Lab Report

471 Words | 2 Pages

The expected results of these crosses if the genes assort independently, are that all the offspring from the P generation would have wild-type allele with red eyes, long wings and dark bodies, the flies would be heterozygous for each allele. If the genes were sex-linked and have females in P-generation, the traits in F1 generation in males would be wild-type. In the reciprocal cross, males would exhibit phenotypes of female if it is mutant phenotypes because only female would inherited her X chromosome to the male in new generation. The adults from the P generation were removed before the F1 generation developed from

Read More

Drosophila Melanogaster Experiment Essay

805 Words | 4 Pages

Finally regarding Hori’s conclusion that Drosophila Melanogaster have lethal effects, while we did not fully test the hypothesis, it was observed that they do tend to avoid high frequency

Read More

Drosophila Melanogaster Lab Report

573 Words | 3 Pages

The purpose of this experiment was to determine if there is natural selection against blind, white-eyed male Drosophila melanogaster, as well as if there is any interplay between selection and drift occurring in populations of different size. This was done by creating and monitoring both small and large populations and placing them in an environment with regular light or complete darkness. It was predicted that natural selection would occur against white-eyed males in the light trials, but would not occur in the dark trials. This selection would occur on the level of male mating success and be seen in a decline in the frequency of Q in the light trials. It was also predicted that drift would be larger in small populations, which would be seen

Read More

White And Sepia Eyes Lab Report

942 Words | 4 Pages

Hypothesis IF white eyes are an autosomal dominant trait AND we cross male flies with autosomal recessive sepia eyes with female flies with white eyes THEN the second generation (F2) offspring will all have white eyes. This hypothesis assumes that both white and sepia eyes are an autosomal gene and that there will be no other genes present that will affect the eye color. The hypothesis predicts that the F1 generation will have only the white eye phenotype, but will be carriers for sepia eyes. With each new generation, the variety of eye color should increase. Graph/Table of Results

Read More

Stable Fly Lab Report

1720 Words | 7 Pages

Akram Alnounou Prof. Molumby BIOS 101 21 September 2017 Answers to Problem Set #1 1. a) The purpose of this experiment was to demonstrate whether or not stable flies will flourish or have a role in rural cow pastures.

Read More

Fruit Fly Lab Report

1536 Words | 7 Pages

The bacteria, which is found in flies’ natural food source rotting fruit, was represented by the yeast, and the sugar was represented by the maple syrup. The purpose was to be able to identify which substances the flies were attracted to the most

Read More

Pied Flycatcher Lab Report

517 Words | 3 Pages

Title: Is Pied Flycatcher mobbing cooperation due to kin selection, reciprocal altruism, or by-product mutualism? Author: Regan Permito Overview: Understanding the complexity of individual and group organismal interactions has presented challenges for scientists since the study of organismal biology began. The theory of evolution employed by natural selection opened up a whole new world when it came to understanding why organisms act as they do.

Read More

More about Drosophila Melanogaster Lab Report

Related Topics

Open Document