Prior to the experiment, two empty vials were prepared the food for the flies: a scoop of dry food was obtained with 1.5ml volumes of water, and soaked for few minutes. Next a few granules of yeast were added and the vials were labeled with the initials, date and the cross (Figure 1). To produce new offspring females needs to be virgin: virgin females were obtained by removing all the adult flies and allowing new generation to develop, it takes approximately 8-10 hours to emerge new flies. When the new generation flies appeared, they were gently tapped to bring the flies to the bottom of the vials and transferred into new vial. Using anesthetize drop, flies were anesthetized and were examined with a dissecting microscope: a soft brush was used to move the flies on the stage of the microscope to determine their phenotype: to produce a new offspring, female flies needed to be virgin, but not the males. …show more content…
The expected results of these crosses if the genes assort independently, are that all the offspring from the P generation would have wild-type allele with red eyes, long wings and dark bodies, the flies would be heterozygous for each allele. If the genes were sex-linked and have females in P-generation, the traits in F1 generation in males would be wild-type. In the reciprocal cross, males would exhibit phenotypes of female if it is mutant phenotypes because only female would inherited her X chromosome to the male in new generation. The adults from the P generation were removed before the F1 generation developed from