Influenza virus is a substantial threat for human health and is a big cause of ~500,000 death rates[1] and is responsible for ~50 million death worldwide over last 100 years.[2]-[4] Influenza viruses is generally classified in to three genera (A, B,C). Influenza A and B viruses have rapid evolution and continuous accumulation of amino acid changes in the antibody-binding sites of the surface protein, results in the change in antigenicity. Despite of existing vaccination programs, research in the area of antigenic rise to predominance.[5],[6]. The global population structure and geographic migration patterns both have an affect to influence the evolution of virus H3N2. The research of Russel et al. points out that East and Southeast