ipl-logo

Ink Lab Report

1217 Words5 Pages

3.6 EXTRACTION OF INK FROM THE SQUID The squid was dissected using a knife on the aluminum dissecting board. The ink was squeezed out from the ink sac which is behind the eyes of the squid. The fresh ink from the squid was collected on a sterile beaker. 3.7 SPREAD PLATE TECHNIQUE The collected fresh ink from the squid was aseptically transferred a drop of inoculum onto the both LA and SWC solidified media. Then, the sterilized hockey stick was used to spread over the ink onto the solidified media. The glass hockey stick was pass through flame before using to ensure aseptic transfer. Then, the petri dish was sealed with parafilm, wrapped with aluminum foil and kept at room temperature for 24 hours. The production of bioluminescent was …show more content…

On a glass slide, 1 drop of distilled water was placed. Then, a loop-full of culture was transferred on the slide and it must be spread. It is then allowed to dry. Then, the smear must be heat fixed by exposing it to flame for few times until it got fixed. It is to prevent the cell from washing away during the staining and washing process. Then, it is air dried and followed by fixing it with flame from Bunsen burner. After fixing the smear, it must be stained using Gram staining solution, firstly crystal violet solution was flood onto it, and allowed for 1 minute, then wash off with tap water. Then, flood the slide with iodine solution for 1 minute and wash it off with tap water again. The formation of a dye-iodine complex will occur in the cytoplasm. Then, it was flooded with ethanol and washed immediately. It is where the process of decolorization occurs. It should not be prolonged as it can over decolorized and immediate washing would sometimes cause under decolorized smear and. Finally safranins were flooded on slide for 30 seconds and rinse it with tap water and the slide must be dried using a blotting paper before viewing and examine in the …show more content…

Lightly smear it on the glass slide. Then, add a drop of 3% hydrogen peroxide (H202) on the bacterial smear on the glass slide. The formation of bubble indicates it is positive for catalase test. If no formation of bubbles, it is a negative test. 3.12.2 Oxidase Test On a filter paper or cotton bud, pick the desired colonies from the agar plate. Then, using a dropper, take the oxidase reagent and drop it on to the filter paper and observe for colour changes. The blue colour appearance indicates positive reaction whereas if there are no changes, then it’s a negative reaction. 3.12.3 Genus Verification using TCBS agar The TCBS medium, known as Thiosulfate Citrate Bile Salts Sucrose Agar is a recommended selective medium which allows the growth of bacteria belonging to the genera Vibrio. TCBS agar was prepared for about 100ml and poured into petri plates, after solidifying, pure colonies of bioluminescent bacteria was streaked. The petri plates were observed after 24 hours. If there is presence of yellow color colonies, it is concluded as Vibrio spp. 3.12.4 Species Verification by Sub-culturing at 4°C using SWC agar media The pure culture of Bioluminescent bacteria was streaked on the agar media and refrigerate at 4°C. The Photobacterium phosphoreum will exhibit slow growth at

Show More
Related
1117 Words | 5 Pages

1 “substrate” and another “ enzyme.” Instead of using the distilled water, this time you are going to use different pH buffer in the enzyme test tube. In the substrate tube, add 7 mL of distilled water, 0.3 mL of hydrogen peroxide, and 0.2 mL of guaiacol for a total volume of 7.5 mL. For the enzyme tube, instead of distilled water add the pH solution (3) and 1.5 mL of peroxidase which equals a total volume of 7.5 mL. Use the dH2O syringe for our pH solution. To clean the syringe, flush it by drawing 6 mL of distilled water.

Read More
449 Words | 2 Pages

Since unknown organism B was gram positive, I had decided my first biochemical test would be the Catalase test per lab manual. The result of the catalase test after adding H2O2 was immediate air bubbles. The second biochemical test performed on organism B was starch agar per lab manual. Once the reagent was added I did not

Read More
1127 Words | 5 Pages

Irrigate your slide with the salt solution. Leave for a few minutes. View one of your slides under low power on

Read More
1200 Words | 5 Pages

The purpose of this lab report is to employ a myriad of skills, tools and, methods learned throughout this semester to perform the appropriate tests for the identification of the assigned unknown bacteria. Add more background information here!!! The most important tools and techniques used during this identification include aseptic technique, microscopic examination and, the use of selective and differential media. Aseptic technique is an important tool for microbiologists. It is imperative that aseptic technique is maintained throughout the length of any test to avoid any cross-contamination that may lead to inaccurate results.

Read More
408 Words | 2 Pages

The unknown bacteria was then tested on multiple selective and differential media. Growth was present on the MacConkey Agar and the colonies were the same color as the plate, which told me my bacteria was gram negative and did not ferment lactose. There was no growth on the Mannitol Salt Agar, and this told me the unknown was not salt tolerant and did not

Read More
1054 Words | 5 Pages

If the catalase enzyme is present in the organism being tested then when in the presence of hydrogen peroxide (H2O2), the enzyme will convert the solution to water and oxygen, this can be observed bubbling of the organism when hydrogen peroxide is added to the test tube. EMB agar is both a selective and differential media; it is selective for gram-negative cells, in that when a gram-positive culture is plated there will be no colonies after incubation because the eosin and methylene dyes prevent the growth of gram-positive organisms, the

Read More
1444 Words | 6 Pages

The tube was placed back in incubation for 96 more hours to observe any more positives. 2.10 Catalase Test A trypticase soy agar plate was used and after incubation, four drops of 3% Hydrogen Peroxide was added to the plate to flow over the bacterial growth. A presence of bubbling was observed. 2.11 Starch Hydrolysis

Read More
771 Words | 4 Pages

My bacterium turned out to be gram positive. When conducting these tests, I only had to do the coagulase test and the catalase test because when doing the catalase test, the reaction was that it had bubbled. If it did not bubble, or have a positive reaction, then I would not have had to do the coagulase test. Also, since my bacterium caused a positive catalase test, I only had to do the coagulase test and no other tests. This is because with staphylococcus organisms, these are the only tests

Read More
901 Words | 4 Pages

Introduction: Enzymes are needed for survival in any living system and they control cellular reactions. Enzymes speed up chemical reactions by lowering the energy needed for molecules to begin reacting with each other. They do this by forming an enzyme-substrate complex that reduces energy that is required for a specific reaction to occur. Enzymes determine their functions by their shape and structure. Enzymes are made of amino acids, it 's made of anywhere from a hundred to a million amino acids, each they are bonded to other chemical bonds.

Read More
1556 Words | 7 Pages

The Cysteine desulfurase test implies that the bacteria can hydrolyze cysteine to produce hydrogen sulfide. Lastly, the Citrate utilizing enzymes test came back positive which indicates that the bacteria has the ability to use citrate as a sole carbon energy source for growth and to produce alkaline

Read More
1061 Words | 5 Pages

The B. Vulgaris samples were approximately 1cm3. They were kept the same size to ensure accurate results. A control test was conducted in distilled water to obtain a result to compare. The ethanol treatments were 40% and 70%. To prepare the solutions a 70% ethanol solution was used to make 40%.

Read More
517 Words | 3 Pages

Being able to identify unknown microbes from systematic testing is what makes the field of microbiology so important, especially in infectious disease control. Using the testing procedure laid out by the microbiology field we are able to identify unknown bacteria present in our everyday lives, and along the way learn a lot about their characteristics that separate them from other types of bacteria. Being able to do this is vital in order for us to understand why microbes are present in certain places, how they are able to grow and what restricts their growth, that way they can be combatted if necessary. These techniques for determining unknowns are also important for isolating and testing infectious disease microbes in order to prevent spreading. Another important aspect of being able to identify unknown microbes is the

Read More
452 Words | 2 Pages

From the Unknown tube professor Cooper gave me, I scratched a little on the slant surface with the sterilized inoculating loop. Then I place it on a clean prepared slide which already had a slight drop of water. The two substances are mixed together in the middle of the slide and let dry completely. One extra step of “heat fix” is necessary to adhere everything to the surface of the slide. To start gram staining, I slightly pour crystal-violet all over the slide and let it sit for 30 seconds before wash it off with water.

Read More
793 Words | 4 Pages

Materials 16 microscopes, worksheets, scratch paper, pencils, safety googles, chemical-resistant gloves, toothpicks, sugar, salt, onion, pencil shavings, corn starch/flour, elodea leaves, Methylene blue, procedures for each station, prepared slides with pencil shavings, corn starch, sugar, salt. Grouping Students will be working in pairs so that they can discuss their observations, and share their thoughts about what they see. In the end of the lesson, they will share their notes with groups and then the whole class. This will help clarify misconceptions.

Read More
1138 Words | 5 Pages

A pathologist examines it and takes measurements, color, shape, etc. Before the tissue is examined by the microscope, the pathologist cuts the specimen into slim portions and stains it with different types of dyes to show parts of the cell. Then, it is viewed in the microscope. There are two ways to observe the specimen (The permanent and frozen section). To create permanent section, the specimen is placed in a fixative so that the cells become fixed and don’t change.

Read More

More about Ink Lab Report

Open Document