Microbiology Unknown Lab Report

Introduction: Microbiology is the study of the microbial world, which is composed of microorganisms that are too tiny to be seen with a naked eye. This project demonstrates the significance of correctly identifying unknown organisms with methods and techniques acquired in the laboratory as they can make or break one's ability to accurately isolate and identify various species. Studying microbes enables oneself to have an insight on not just their structure or how they function, but how they have an impact on organisms and their environment around them as they can be found nearly anywhere. The importance of the process of identifying unknown bacteria vary from determining the causative agent of a disease, to testing if a specific food or fluid is safe to consume, and simply knowing what is present

The presence of nitrate reductase also came back positive, this implies that the microbe is capable of reducing nitrate to nitrite. The catalase displays that the bacteria is able to break down the toxic forms of oxygen as well. However, the Oxidase test indicates that the microbe is unable to produce cytochrome c oxidase. The MR-VP test shows that the bacteria can carry out mixed acid fermentation when supplied with glucose but is incapable of 2,3 butanediol fermentation after the following mixed-acid fermentation. The tryptophanase test indicates that the bacteria is unable to synthesize tryptophanase in order to produce indole. The Cysteine desulfurase test implies that the bacteria can hydrolyze cysteine to produce hydrogen sulfide. Lastly, the Citrate utilizing enzymes test came back positive which indicates that the bacteria has the ability to use citrate as a sole carbon energy source for growth and to produce alkaline