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Fermentation by yeast lab results
Fermentation by yeast lab results
Fermentation by yeast lab results
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We got negative for indole (no production of indole, pyruvic acid and ammonia), negative for Methyl Red (our bacteria does not perform mixed-acid fermentation when supplied glucose), negative for Voges-Proskauer (no fermentation of glucose in order to produce 2,3-Butanediol-Butanediol fermentation), but positive for Citrate utilization, which means our bacteria uses citrate as a sole carbon source and energy. Something interesting here is that according to the lab textbook organism that degrade citrate must also use ammonium salts, and in the process, they produce ammonia that causes the medium to become alkaline (under this condition the medium turns to deep Prussian blue, indicating the utilization of citrate). The genus Alcaligenes is well known for being alkali-producing
Dependent Variable amount of product (glucose and fructose) produced 2. Independent Variable temperature 3. Controlled Variables pH, amount of substrate (sucrose) present, sucrase + sucrose incubation time Effect of Substrate Concentration on Enzyme Activity 1. Dependent Variable amount of product (glucose and fructose) produced 2.
I predicted that the control would have a higher alcohol content than the experimental since beta and alpha amylase are working together. Since only Alpha-Amylase worked in the experimental, there was probably bigger carbohydrates present in the flask, therefore, there was a lower alcohol percentage since yeast can’t digest bigger sugars. b. My results also matched my prediction regarding mean reducing carbohydrate levels during the mashing process between the control and the experimental. My prediction stated that there would be less reducing carbohydrate ends in the experimental, which was proven in the data table.
LABORATORY REPORT Activity: Enzyme Activity Name: Natalie Banc Instructor: Elizabeth Kraske Date: 09.22.2016 Predictions 1. Sucrase will have the greatest activity at pH 6 2. Sucrase will have the greatest activity at 50 °C (122 °F) 3. Sucrase activity increases with increasing sucrose concentration Materials and Methods Effect of pH on Enzyme Activity 1. Dependent Variable amount of product (glucose and fructose) produced 2.
Many organisms use energy to perform their cellular functions. That energy comes from the energy that is stored in food then converted to adenosine triphosphate or ATP. ATP can be obtained with or without oxygen, aerobic respiration and anaerobic respiration. Aerobic respiration produces carbon dioxide (CO2) as a by-product while anaerobic respiration produces Ethanol (C2H6O) or Lactic acid (C3H6O3). In aerobic respiration the “CO2 produced during cellular respiration can combine with water to produce carbonic acid.”
This catalyst is necessary for an aerobic organism to survive in aerobic conditions. After the experiment it was observed that my Gram positive organism did in fact bubble and utilize catalase. The third test conducted was on the MSA agar. This test was conducted for the purpose of selective and differential whether or not my organism can tolerate high salt concentrations. It is based on the mannitol fermentation.
Salmonella typhimurium account 1,2-propanediol, de-oxy sugars. Identify an equimolar extent of 1, 2-propanediol when (methyl pentose), rhamnose or fucose was used as substrate. However, still 1, 2-propanediol is not further metabolized by anaerobic cultures, and will disappear gradually from the middle in s. typhimurium cultures maintained in similar surroundings. This meticulous inquiry discovered that when grown on rhamnose, s. typhimurium excreted 1.0 M 1, 2-propanediol/m of sugars in the middle. Now, after collapse of the sugar, the diol concentration has arrived to its maximum and step by step it departed when the culture was kept in the same conditions for more time.
The Effect of Sugar Concentration on CO2 Production by Cellular Respiration in Yeast Introduction In this lab, our main focus was to find how sugar concentration affect yeast respiration rates. This was to simulate the process of cellular respiration. Cellular respiration is the process that cells use to transfer energy from the organic molecules in food to ATP (Adenosine Tri-Phosphate). Glucose, CO2, and yeast (used as a catalyst in this experiment) are a few of the many vital components that contribute to cellular respiration.
Microorganism - can cause illness and is hard to detect. Oxidation - what happens when oxygen meets other substances. Fungus - it is mold, yeast, and bacteria.
Only the heated solution caused the balloon to expand, suggesting that the increase in temperature is linked to the balloon’s expansion. Furthermore, as the solution was only heated to 60°C, no water vapor was produced to fill the balloon, suggesting the gas was produced solely by the yeast. Thus, the yeast reacted to the heat, supporting the claim that yeast can respond and is alive. Sources of error in this experiment could have included incorrect preparation of solutions. The solutions of yeast, water, and sugar, could have been measured incorrectly causing the control and experimental solutions to be different.
The Effect of Temperature on the Amount of Oxygen Consumed During Cellular Respiration of Saccharomyces Cerevisiae Purpose: To determine the temperature at which baker’s yeast (Saccharomyces cerevisiae) respires most efficiently in order to produce the best baked goods as possible when utilizing yeast. Research Question: How does temperature affect amount of oxygen used during cellular respiration of yeasts? Introduction: Respiration is process of releasing energy from organic compounds in order to produce energy.
There are several reactions occur when there is plenty of oxygen present. Then the energy released is used by the yeast for growth and activity. However, when the oxygen supply is limited, the yeast can only partially breakdown the sugar. Alcohol and carbon dioxide are produced in this process known as alcoholic fermentation. The fermentation occur when the carbon dioxide produced in these reactions.
For example, fermentation occurs in yeast in order to gain energy by transforming sugar into alcohol. Fermentation is also used by bacteria, they convert carbohydrates into lactic acid. Ethanol fermentation is done by yeast and certain bacteria, when pyruvate is separated into ethanol and carbon dioxide. Ethanol fermentation has a net chemical equation: C6H12O6 (glucose) > 2C2H5OH (ethanol) + 2CO2 (carbon dioxide). This process of ethanol fermentation is used in the making of wine, bread, and beer.
Abstract — This experiment was conducted to familiarize the students with the procedures regarding distillation—to be more precise, the separation of ethanol from an alcoholic beverage—using a distillation set-up consisting of boiling chips, a Bunsen burner, a condenser, a thermometer and several other materials. In the end, it was discovered that one may actually separate a homogeneous mixture, given that the components of said mixture differ in volatility and that they utilize a complete distillation set-up and follow laboratory safety rules and regulations. Keywords — Matter, homogeneous and hetereogeneous mixtures, distillation, volatility, boiling point I. INTRODUCTION There are typically two categories of matter, these are pure substances
Background Information: Yeast fermentation is directly affected by the change in temperature, because the rate of chemical reactions is affected by temperature. If the yeast has been exposed to its optimum temperature (66.667 degrees Celsius) then it will give off the highest carbon dioxide production. As the temperature gets higher, the yeast will produce more carbon dioxide, until at some point carbon dioxide production will decrease, that is when the yeast cells have become denatured due to the increase in temperature. Chemical reactions