ipl-logo

Unknown Samples Through Staining And Chemical Test Lab Report

2054 Words9 Pages

Analysis of Unknown Samples Through Staining and Chemical Testing (Unknown 8) Abstract: Identifying bacteria by culture is possible due to various tests ranging from staining techniques to biochemical testing. Bacteria have a wide range of variances. To help identify unknown bacteria, gram staining, selective and differential media, analytical profile index (API), and BLAST tests were performed. It resulted in gram negative, rod shaped, lactose fermenting bacteria. The most likely bacterium it could be is Klebsiella pneumoniae. Introduction: Through various experiments performed over a period of time, it is possible to identify an unknown sample of bacteria. Three were performed in this experiment: The Gram Stain is a differential staining technique that allows the viewer to see if …show more content…

The morphology of the bacteria is a rod shape. Through this experiment, it can be concluded that the bacteria is a Gram negative species. The selective and differential plates were left to incubate and after viewing the results, there was no growth on either of the plates. On the MAC plate, there is a bit of discoloration of the phenol red pH indicator. Though there was a bit of discoloration, it is not a strong enough indication that the bacteria reacted with the agar because there was no growth. The MSA plate also showed discoloration throughout the plate and shows a stronger indication in comparison to the MAC plate shown in Figure 2. Though there was no growth on the plate, the strong discoloration indicates that the bacteria reacted with the sodium chloride in the agar. The API test resulted in mostly negative results in amino acid catabolism and mostly positive in fermentation. As seen in Figure 3, the results of the fermentation processes were not strong, but they were

Show More
Related

Alcaligenes Faecalis Experiment

636 Words | 3 Pages

According to the series of test that my group ran for our unknown specimen, we had a match with the bacteria known as Alcaligenes Faecalis. This bacterium belongs to one of the major group of gram-negative bacteria (Phylum Proteobacteria). Alcaligenes Faecalis (Genus, species) is a rod shaped (bacillus), 0.5-1.2 x 1.0-3.0 µm, round with scalloped margin (colony configuration growth), motile (with one to nine peritrichous flagella), gram-negative, non-fermentative bacteria, obligate aerobic, having oxygen as the principal terminal electron acceptor in the electron transport chain (ETC). We consider we have a match with the species Alcaligenes Faecalis because of the following reasons: Fermentation tests performed (Durham sugars) were negative, which indicate that our bacteria use a different metabolic means for growth (non-fermentative gram-negative bacteria).

Read More

Unknown Bacteria Lab Report

1200 Words | 5 Pages

The purpose of this lab report is to employ a myriad of skills, tools and, methods learned throughout this semester to perform the appropriate tests for the identification of the assigned unknown bacteria. Add more background information here!!! The most important tools and techniques used during this identification include aseptic technique, microscopic examination and, the use of selective and differential media. Aseptic technique is an important tool for microbiologists. It is imperative that aseptic technique is maintained throughout the length of any test to avoid any cross-contamination that may lead to inaccurate results.

Read More

Unknown Lab Report Essay

408 Words | 2 Pages

The unknown bacteria was then tested on multiple selective and differential media. Growth was present on the MacConkey Agar and the colonies were the same color as the plate, which told me my bacteria was gram negative and did not ferment lactose. There was no growth on the Mannitol Salt Agar, and this told me the unknown was not salt tolerant and did not

Read More

Unknown Lab Report

1444 Words | 6 Pages

These microorganisms are used to teach us how multicellular organisms came to be and how they can survive today. These small, microscopic organisms are so unique that the identification of them is paramount in the advancements of science. Knowing the chemical makeup, the shape, and the biochemical processes is important in identifying these organisms to understand how they survive and where. A number of tests can be ran on an unknown bacteria to determine their ideal

Read More

Ca-Mrsa Case Studies

1264 Words | 6 Pages

In lab, we learned to distinguish two types of bacteria using the gram stain, gram positive and gram negative. Since S. Aureus has a thick peptidoglycan cell wall with teichoic acid, it stains gram positive. Further tests will indicate the bacteria, including the presence of catalase, sheep blood hemolysis, mannitol fermentation, halotolerance, and coagulase, which S. Aureus is positive for all. 3.)

Read More

Unknown Lab Report

1706 Words | 7 Pages

The student is to use studied tests to eliminate possible generas and to isolate the correct genus and identify it to the given unknown. This proves the diversity of microorganisms and their uniquely varying traits. The resulting data obtained in class will enhance students’ laboratory skills and knowledge regarding microbial laboratory. Introduction

Read More

Sample Lab Report

488 Words | 2 Pages

Major unknown #202 was given out by the instructor, and the unknown bacterium was streaked out on a Trypticase Soy Agar tube and plate to inoculating the bacterium and incubating. After incubated and grown the morphology was observed and several Gram stains were performed to determinate if the bacterium were gram positive or negative, and the morphology of the bacterium. The Gram Stain of my major unknown #202 was determinate to be Gram negative bacilli, and was double checked by the Gram check slide. Also I noticed that my bacterium was a facultative anaerobe and according to my results of endospore test, my bacterium has not endospores. So according to the list of possible major unknowns provided by the instructor, I narrow my bacterium thru

Read More

Unknown Bacteria By Gram Staining And A Series Of Biochemical Test

1383 Words | 6 Pages

The main purpose of this study is to identify the given unknown bacteria by gram staining and a series of biochemical test. There are many types of bacteria and they produce different enzymes. However, Some bacteria produces same enzymes, so specific tests were done to identify the particular enzymes. There are four main types of Gram-negative bacteria, which are Escherichia coli, Klebsiella pneumonia, Enterobacter aerogenes, and Salmonella typhimurium. Therefore, the unknown could be any of these four bacteria’s, so the main goal of this experiment is to identify the particular unknown bacteria.

Read More

Gram Test Lab Report

836 Words | 4 Pages

The EMB plate served two purposes. It inhibited gram positive growth, and tested for the organisms ability to ferment lactose. After 48 hours the inoculated EMB plate presented with strong, but colorless growth. This result reconfirmed that the organism was gram negative, it also determined that the organism was non-lactose fermenting. The possible organisms were now limited to Proteus mirabilis, Proteus vulgaris, Moraxella morganii, Providencia stuartii, Serratia marcescens, Pseudomonas aeruginosa, and Alcaligenes faecalis.

Read More

Should The Cuti Plates Be Incubated At And Why?

739 Words | 3 Pages

What information does this tell you about the bacteria in the sample, and why did the colony change colour? [2 marks] This tells me that the bacteria in the sample contains coliforms such as Escherichia coli (E.coli). The colonies changed colour as the enzymes produced by the bacterial cells reacted with the red galactosidase in the agar medium, this reaction causes the colonies to turn pink making them easily

Read More

Microorganism Lab Report

1350 Words | 6 Pages

Microbiology lab serves to teach students basic knowledge of concepts, techniques, and tests used by scientists in the laboratories. Thus far, various methods have been learned to identify and distinguish between microorganisms. The purpose of this exercise is to logically outline a plan using techniques and examinations (learned throughout the semester), critical thinking, and application of that knowledge to identify an unknown bacterium from a mixed culture. This report covers the logical progression used to identify Unknown 15, the isolated microorganism. Handling of microorganisms in the lab requires the use of aseptic technique to establish pure cultures and to protect against environmental contamination.

Read More

Blood Agar Lab Report

1057 Words | 5 Pages

After a gram stain was done unknown #257 was identified as a gram positive organism because when observed under the microscope the organism appeared purple with cocci in clusters. The organism was also catalase positive which means that it produced enzyme catalase and bubbled when hydrogen peroxide was added to it. Three test were conducted based on the result of the gram staining procedure. Blood agar with a Novobiocin disk was chosen as well as DNase (DNA) and Mannitol Salts (MSA) agar. The Blood agar is a bright red, opaque plate and the streaking or the inoculation technique was a modified streaking for isolation with a heavy quadrant one.

Read More

Nature And Importance Of Pseudoma

1723 Words | 7 Pages

It is rod shaped in structure and measures 0.5 to 0.8 µm by 1.5 to 3.0 µm. This bacteria is useful to microbiologists and

Read More

Biochemical Test Lab Report

1371 Words | 6 Pages

Biochemical tests are the tests used for the identification of bacterial species based on the differences in the biochemical activities of different bacteria. Bacterial physiology differs from one species to the other. These differences in carbohydrate metabolism, protein metabolism, fat metabolism, production of certain enzymes and ability to utilize a particular compound help them to be identified by the biochemical tests. Gram’s stain was originally devised by histologist Hans Christian Gram in 1884. Gram-positive bacteria stain purple, while Gram-negative bacteria stain pink when subjected to Gram staining.

Read More

Essay On Microorganism

1328 Words | 6 Pages

Introduction In the past, it has been vital to distinguish the identities of microorganisms in the world. Knowing their identity has aided in diagnosing numerous diseases and has discovered the most beneficial treatment. Lately, it has been vital to differentiate the identities of microorganisms worldwide.

Read More

More about Unknown Samples Through Staining And Chemical Test Lab Report

Open Document