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Gram Test Lab Report

836 Words4 Pages

INTRODUCTION Discovering an unknown organism or pathogen is a very common project done in microbiology classes. The study of microbiology requires not only knowledge about various types of organisms but also requires a practical knowledge on how to identify those organisms. This project utilizes students abilities to apply the practical knowledge they have learned. At the beginning of the project the professor presented each student with a numbered test tube. The possible organisms were Escherichia coli, Enterobacter aerogenes, Proteus mirabilis, Citrobacter freundii, Moraxella morganii, Providencia stuartii, Serratia marcescens, Citrobacter freundii, Pseudomonas aeruginosa, Alcaligenes faecalis, Enterococcus faecalis, Micrococcus luteus, …show more content…

The gram stain was done to identify whether the organism was a gram positive or gram negative organism. A few colonies were selected from the nutrient agar. The steps of the gram stain were executed. The stain resulted in gram negative rods. This result eliminated all gram positive organisms. Leaving the possible organisms to be Escherichia coli, Enterobacter aerogenes, Proteus mirabilis, Citrobacter freundii, Moraxella morganii, Providencia stuartii, Serratia marcescens, Citrobacter freundii, Pseudomonas aeruginosa, or Alcaligenes …show more content…

The EMB plate served two purposes. It inhibited gram positive growth, and tested for the organisms ability to ferment lactose. After 48 hours the inoculated EMB plate presented with strong, but colorless growth. This result reconfirmed that the organism was gram negative, it also determined that the organism was non-lactose fermenting. The possible organisms were now limited to Proteus mirabilis, Proteus vulgaris, Moraxella morganii, Providencia stuartii, Serratia marcescens, Pseudomonas aeruginosa, and Alcaligenes faecalis. Following the EMB test, a TSI agar was inoculated. The TSI agar was used as a differential medium to determine the organisms ability to ferment lactose, sucrose, and glucose. The agar was also used as a PH indicator. The TSI was read after 48 hours, its butt was red, as well as its slant. This result reconfirmed that the organism did not ferment lactose, as well as it did not ferment sucrose, or glucose. This test also determined that the organism was alkaline. This limited the possible organisms to only Pseudomonas aeruginosa, and Alcaligenes

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