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Sample Lab Report

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Major unknown #202 was given out by the instructor, and the unknown bacterium was streaked out on a Trypticase Soy Agar tube and plate to inoculating the bacterium and incubating. After incubated and grown the morphology was observed and several Gram stains were performed to determinate if the bacterium were gram positive or negative, and the morphology of the bacterium. The Gram Stain of my major unknown #202 was determinate to be Gram negative bacilli, and was double checked by the Gram check slide. Also I noticed that my bacterium was a facultative anaerobe and according to my results of endospore test, my bacterium has not endospores. So according to the list of possible major unknowns provided by the instructor, I narrow my bacterium thru …show more content…

So the results were sulfur: positive, indole: Negative, and for medium: medium motility. And the results indicated that could be Klebsiella pneumoniae but also it could be Enterobacter aerogenes. To confirm the deduction of hat my bacterium could be Klebsiella pneumoniae I decided to do my fourth test. And the Urea Hydrolysis and if the results turn negative the bacterium will be Enterobacter aerogenes, or if it turns positive the bacteria will be Klebsiella pneumoniae. The results turns pink after 48 hours of incubation and this indicated that the Urea Hydrolysis it is positive (slow). As a result this test confirm that my bacterium is Klebsiella pneumoniae. Klebsiella pneumoniae is a Gram negative rod bacterium that can form a capsule. K. pneumoniae can be found in the GI tract in humans and it can cause nosocomial urinary tract infections and pneumonia also it can be cause death. K. pneumoniae can be contracted be direct contact. Washing hands before and after had a direct contact with a patient can avoid and decrease the spread of K.

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