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Sample Lab Report

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Major unknown #202 was given out by the instructor, and the unknown bacterium was streaked out on a Trypticase Soy Agar tube and plate to inoculating the bacterium and incubating. After incubated and grown the morphology was observed and several Gram stains were performed to determinate if the bacterium were gram positive or negative, and the morphology of the bacterium. The Gram Stain of my major unknown #202 was determinate to be Gram negative bacilli, and was double checked by the Gram check slide. Also I noticed that my bacterium was a facultative anaerobe and according to my results of endospore test, my bacterium has not endospores. So according to the list of possible major unknowns provided by the instructor, I narrow my bacterium thru …show more content…

So the results were sulfur: positive, indole: Negative, and for medium: medium motility. And the results indicated that could be Klebsiella pneumoniae but also it could be Enterobacter aerogenes. To confirm the deduction of hat my bacterium could be Klebsiella pneumoniae I decided to do my fourth test. And the Urea Hydrolysis and if the results turn negative the bacterium will be Enterobacter aerogenes, or if it turns positive the bacteria will be Klebsiella pneumoniae. The results turns pink after 48 hours of incubation and this indicated that the Urea Hydrolysis it is positive (slow). As a result this test confirm that my bacterium is Klebsiella pneumoniae. Klebsiella pneumoniae is a Gram negative rod bacterium that can form a capsule. K. pneumoniae can be found in the GI tract in humans and it can cause nosocomial urinary tract infections and pneumonia also it can be cause death. K. pneumoniae can be contracted be direct contact. Washing hands before and after had a direct contact with a patient can avoid and decrease the spread of K.

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Unknown #10 produced no identifiable macroscopic characteristics as a broth, so the first step was to Gram stain a loopful to determine the microscopic characteristics. Gram staining not only helped identify Unknown #10’s microscopic morphology but it also helped ensure the specimen was a pure culture—no other bacteria were visible when Unknown #10 was Gram stained and observed under the microscope. Unknown #10’s key microscopic morphology was that it was a very small, Gram negative bacillus. Though bacilli can possibly form endospores, no empty white centers were visible which suggested that Unknown #10 was not an endospore forming bacteria. No quick endospore stain was performed to validate this assumption since only one assigned organism was endospore forming and unlike Unknown #10, that organism was Gram positive.

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Exercise 14: Unknown Identification Lab Report The purpose of the study was to identify the unknown bacterium using various biochemical tests in addition to using scientific methods in determining the outcome of the hypothesis. Each biochemical test will help determine the bacteria based on specific characteristics of each organism. I was giving unknown number 232. The first procedure that needed to be done after obtaining unknown bacterial mixture was to isolate the two bacteria in a pure culture using the streak plate method described in Microbiology Laboratory Manual Eight Edition. The material used was trypticase soy agar (TSA) plate, nutrient plate, starch agar, hydrogen peroxide, iodine reagent and microscope.

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The unknown bacteria was then tested on multiple selective and differential media. Growth was present on the MacConkey Agar and the colonies were the same color as the plate, which told me my bacteria was gram negative and did not ferment lactose. There was no growth on the Mannitol Salt Agar, and this told me the unknown was not salt tolerant and did not

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Figure 3. Testing of transformed and mutant bacteria on minimal medium Growth was observed on the Transformed (Trsf) section and not on the Mutant (Mut)

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Genetic engineering is changing the DNA code to express different traits. A plasmid is a circular piece of DNA that contains important genetic information. Recombinant DNA is the product after inserting your desired genes. The genes we hoped to insert in the pGLO lab were the GFP gene and the ampicillin resistance gene. GFP was needed so that we would tell if the ampicillin resistance gene had been properly placed when the bacteria glowed under a UV light.

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771 Words | 4 Pages

My bacterium turned out to be gram positive. When conducting these tests, I only had to do the coagulase test and the catalase test because when doing the catalase test, the reaction was that it had bubbled. If it did not bubble, or have a positive reaction, then I would not have had to do the coagulase test. Also, since my bacterium caused a positive catalase test, I only had to do the coagulase test and no other tests. This is because with staphylococcus organisms, these are the only tests

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the patient should stop taking any antibiotics such as Pepto-Bismol, and proton pump inhibitors (PPIs) for two weeks before the test. Then, during the test, special solution contains urea that breaks down protein should be swallowed. The urea used is harmless and radioactive at the same time. Then, the specialist will recognize the presence of H. pylori. In existence of bacteria, carbon dioxide will be noticed and recorded in exhaled breath ten minutes later.

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In order to determine of his was an experimental error or a new form of bacteria, we swabbed this bacteria into four new cultures and retested them. The new bacteria had grown back normally. We determined that there could have been experimental error by not keeping the testing area completely sterile. Also, we determined that the E. Coli bacteria could have just grown with abundance in these particular cultures. In this case, it would been helpful to test more than two cultures for each

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Being able to identify unknown microbes from systematic testing is what makes the field of microbiology so important, especially in infectious disease control. Using the testing procedure laid out by the microbiology field we are able to identify unknown bacteria present in our everyday lives, and along the way learn a lot about their characteristics that separate them from other types of bacteria. Being able to do this is vital in order for us to understand why microbes are present in certain places, how they are able to grow and what restricts their growth, that way they can be combatted if necessary. These techniques for determining unknowns are also important for isolating and testing infectious disease microbes in order to prevent spreading. Another important aspect of being able to identify unknown microbes is the

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The bacterium was determined to be Gram- negative rods. The first attempt of gram stain the unknown appeared to look positive and cocci. But, the next day onwards for 4 consecutive days the unknown showed up as a gram- negative gram stain and appeared pink rods. Triple Sugar Iron Agar is a differential medium. It differentiates Gram-negative enteric bacteria based on the ability to reduce sulfur and ferment carbohydrates (lactose, sucrose, and glucose).

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872 Words | 4 Pages

The cells of the bacteria were rod shaped, therefore, their morphology was bacillus. The following test I did was the Oxidase test. The unknown tested negative for this particular test because the oxistrip failed to turn blue shortly

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The earliest descriptions of Klebsiella pneumoniae

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After experiment on microscope under oil immersion, I learned that my Unknown is gram positive. Under the lens, the bacteria appears in purple color. Its morphology is cocci arranged in cluster. However, during decolorizing process, I put too much alcohol to the crystal violet-iodine complex making the color overly removed. That led to the result of my gram positive has slightly redish

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528 Words | 3 Pages

If the urine lab results show that the urine is too concentrated, this could be an indication that the patients kidneys are not functioning properly. If your lab results showed your urine had a pH of 6, then that is a great sign because that is the average pH in healthy individuals. Although your pH was in what is considered a normal range, the turbid appearance and amber color suggests that you need to increase the amount of water that you are drinking daily. Your lab results showed that there was no protein in your urine. It is important that a patient would have no protein in the urine because then they would have something called proteinuria.

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Kristen Johns BIOL 2164 Disease Report 10/22/15 Klebsiella Pneumoniae Klebsiella pneumonia or also known as Friedlanders bacillus is a disease that is found in humans and animals. The genus is named after Edwin Klebs a 19th century German Microbiologist. Klebsiella begin in soil and the water on plants. K. pneumonia is found in the digestive and respiratory systems causing lobar pneumonia, meningitis, urinary tract infections, and would infections.

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