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E. Coli Bacteria

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Although the nature of this kind experiment was unable to provide us with numerical data, our graph was constructed using the average percent of E. Coli killed for each pair of cultures. To determine these percents for each substance we used the process of elimination. We put the two petri dishes side by side and used a checklist ranging from 1-100% to narrow down the possibilities. After obtaining a small range of possible percents, we made an estimate to determine the percent. After doing this for both the of cultures for that pair we averaged the two numbers. We repeated this for all of the culture pairs in our experiment. Our data showed us how effectively each antiseptic killed the E. Coli bacteria. For the control cultures we saw no decrease in E. …show more content…

In order to determine of his was an experimental error or a new form of bacteria, we swabbed this bacteria into four new cultures and retested them. The new bacteria had grown back normally. We determined that there could have been experimental error by not keeping the testing area completely sterile. Also, we determined that the E. Coli bacteria could have just grown with abundance in these particular cultures. In this case, it would been helpful to test more than two cultures for each …show more content…

The triclosan antiseptic is the most effective killer of E. Coli bacteria; however, recent studies have shown it could be harmful to human health. We recommend to doctors, hospitals, schools and families to use non- triclosan based products to help kill bacteria. Although the most effective killer, triclosan could potentially contribute to the development of antibiotic-resistant germs and be harmful to the immune system. Scientists must find the balance between effectively killing bacteria and the safety of human

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Gram positive organisms are susceptible to antibiotics such as penicillin (Introduction to Penicillins, 2012). This is because, gram positive bacteria have a thick layer of peptidoglycan, a sugar and peptide coating that gives a cell its shape and helps it stay intact (Wiley, 2004). The original antibiotic, penicillin, and the likes are used to treat infections caused by bacteria that are gram positive. Based on the scope of the experiment and the materials needed, a budget estimate of $1,000.00 would be needed to buy reagents and materials for this experiment.

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Methods Unknown microbial #398 went through several of tests in order to identify its characteristics when isolated from a urine sample of Doris, a 64- year old patient with a kidney infection. To identify unknown #398, must prepare a working and a reserve stock by the inoculation from a broth culture and by quadrant streaking method on a PEM and EMP plates. The following test procedures were incubated at 37°C for 48 hours for observation and identification for unknown #398. The identification of unknown #398 followed test procedures from Brown1.

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Exercise 14: Unknown Identification Lab Report The purpose of the study was to identify the unknown bacterium using various biochemical tests in addition to using scientific methods in determining the outcome of the hypothesis. Each biochemical test will help determine the bacteria based on specific characteristics of each organism. I was giving unknown number 232. The first procedure that needed to be done after obtaining unknown bacterial mixture was to isolate the two bacteria in a pure culture using the streak plate method described in Microbiology Laboratory Manual Eight Edition. The material used was trypticase soy agar (TSA) plate, nutrient plate, starch agar, hydrogen peroxide, iodine reagent and microscope.

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The purpose of this lab report is to employ a myriad of skills, tools and, methods learned throughout this semester to perform the appropriate tests for the identification of the assigned unknown bacteria. Add more background information here!!! The most important tools and techniques used during this identification include aseptic technique, microscopic examination and, the use of selective and differential media. Aseptic technique is an important tool for microbiologists. It is imperative that aseptic technique is maintained throughout the length of any test to avoid any cross-contamination that may lead to inaccurate results.

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The unknown bacteria was then tested on multiple selective and differential media. Growth was present on the MacConkey Agar and the colonies were the same color as the plate, which told me my bacteria was gram negative and did not ferment lactose. There was no growth on the Mannitol Salt Agar, and this told me the unknown was not salt tolerant and did not

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The sterile cotton swab was inserted in the S. epidermidis culture and twirled around to obtain a specimen. The entire plate was inoculate with the swab from top to bottom, to achieve a lawn of growth. The dry forceps was used to remove the antibiotic disk into the appropriate spot on the plate. This process was repeated for the all antibiotics with aseptic technique being used. The plate was incubate with lid up on the bookshelf at room temperature for 48 hours.

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Conclusion In conclusion carprofen has no significant effect on bacterial treatment. Carprofen was given late into the acute phase reaction of the induced E.coli when clinical signs were first seen. Treatments with carprofen had modulatory effects on clinical and immunological parameters.

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Escherichia Coli are bacteria found in the environment, in contaminated food and beverages and in intestines of mammals. It is the most widespread bacteria in the family of gram-negative bacteria (bacteria that can easily enter the host either by urinary and existing catheters, respirators or even wounds) called enterobacteriaceae. It is first discovered in 1885 by German bacteriologist Theodor Escherich. He was devoted in finding out what causes fatal intestinal diseases in children. He experiments by studying fetal feces through recording the organisms that are present in the intestines of healthy children and, comparing them with organisms found in sick children.

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An example of this includes a highly publicized case of over 625 people becoming ill off of Jack in the Box hamburgers contaminated with Escherichia coli (E. coli O157: H7) and in this food poisoning epidemic four children died. The largest outbreak so far of E. coli O157: H7 occurred in 1996 in Japan with radishes. In this enormous case, 9,500

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Then 1 ml from these bacterial solutions were added to 1 ml of MHII containing different concentration of ciprofloxacin 0.5x, 1x, 5x, 10x, 20x, 30x, 50x,75x and 100x MIC to each well in 12-well plate. Colony counts (CFU/ml) were determined at different time points (T0, T3, T24) by using appropriate dilutions of each culture. Using spot-plating method (32) 10µl was spotted on LB agar plates. However after 24 hour exposure the bacterial cell from dilutions of ≤ 1x102 were washed twice with sterile PBS prior to plating in triplicate. These were then incubated overnight at 37°C.

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Should mandatory antimicrobial stewardship programs set in place to control antibiotic use to decrease the chances of superbugs to emerge? Table of contents Introduction 4 Biological background 5 Alternative views for implementing antimicrobial stewardship programs 6 Alternative views against implementing antimicrobial stewardship programs 6 Conclusion 7 Bibliography 8 Introduction The World Health Organisation has announced antibiotic resistance and the rise of superbugs as a threat to human race. Superbugs are defined as bacteria equipped with antibiotic resistance genes. The affected bacteria can transfer the antibiotic resistance genes to other bacteria and completely paralyse humans to combat against bacterial infections (News Limited 2014). According to recent studies

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the objective function was Equation (1-4). The goal of this optimization was to achieve a situation of independent variables (field strength, retention time, power of ultrasound and sonication time), in which with the lowest energy consumption, the amount of E. coli in the sample is equal to zero. Boundary conditions specified in optimization was that the values of the independent variables are in the range of the experiment. All independent and dependent variables by reason of the same importance, the weight of one was given, and at the end, minimizing the value of the objective function was selected (Table 4-2). The optimum values for field strength, retention time, power of ultrasound and sonication time were equal to 10 kv/cm, 35s, 185.90 w, 4.47 min, respectively.

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Tetracyclines prevents protein synthesis in bacteria. Some antibiotics can kill both good and bad cells so it is important for providers to know the patient’s correct diagnosis and health history as to not allow

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Escherichia Coli 0157: H7 This paper will specialize on a specific type of bacterial foodborne illness caused by the bacteria Escherichia Coli. E. coli was discovered by Theodore von Escherich in 1885. E.coli is a natural found bacteria that lies throughout the intestinal tract of warm blooded animals and comes in many forms only one of which is deadly. This form is E. coli 0157:H7 which can be caused by direct exposure to fecal matter to kill this rouge

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Stewardship programs can monitor the prescription of antibiotic to prevent the unnecessary use which will lead to resistance. The laboratory processing and providing test results must provide accurate results quickly. Research towards better the following areas will slow the increase of antibiotic resistance in bacteria. Introduction: twenty-three thousand people die

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