In this lab, the oxidation of a secondary alcohol was performed and analyzed. An environmentally friendly reagent, sodium hypochlorite, was used to oxidize the alcohol, and an IR spectrum was obtained in order to identify the starting compound and final product. The starting compound could have been one of four alcohols, cyclopentanol, cyclohexanol, 3-heptanol, or 2-heptanol. Since these were the only four initial compounds, the ketone obtained at the end of the experiment could only be one of four products, cyclopentanone, cyclohexanone, 3-heptanone, or 2-heptanone. In order to retrieve one of these ketones, first 1.75g of unknown D was obtained.
Title: How Ph Levels Affected the Fermentation of Beer Hypothesis: The beer will be left with more sugar deposit as the Ph levels increase because alpha/beta -amylase will no longer function. Predictions: Alcohol Percentage Analysis for the Control and the Experimental During this experiment, the pH level was increased, therefore Alpha-Amylase was favored. Due to the nature of Alpha-Amylase cutting randomly through a large carbohydrate molecule, it leaves bigger sugars in the flask, which cannot be digested by yeast. Due to this, less reactions should occur in the experimental, therefore leading to a lower percentage of alcohol production, compared to the control.
Therefore, acid fat, lactose fermentation, mannitol fermentation were not needed to be performed, because they are selective to a specific thing. As a result, Unknown bacteria “W” was concluded to be gram stain positive, endospore positive, bacilli shape (rod shape), and arranged in chains (strepto-). Test Purpose Reagents Observation Results Gram Stain To determine gram reaction of bacteria.
Another hypothesis made was that the bacteria would glow with the addition of the sugar arabinose. All three of the objectives seem to go hand in hand. The lab began by inserting transformation
Yeast is alive because it can to metabolize and respond to environmental changes. The purpose of the first experiment was to determine whether yeast can metabolize. The bromothymol blue solution with yeast changed from blue to yellow. Bromothymol blue is an acid-base indicator that turns yellow in the presence of acid. The color change indicates that carbonic acid was formed from the reaction of water and carbon dioxide, a byproduct of metabolization.
Joshua Miller 12/18/17 Fermentation Lab report Introduction The term fermentation refers to the chemical breakdown of a substance by bacteria, yeasts, or other microorganisms, typically involving effervescence and the giving off of heat (wikipedia). Sugars are converted to ethyl alcohol when fermentation happens. In this experiment we determined if yeast cells undergo fermentation when placed in a closed flask with no oxygen. Glucose and yeast are mixed together in a closed flask and allowed to incubate for about one hour.
1% glucose, 1% maltose and 1% lactose all progressively get positive results by changing colours to reddish brown at the end of this experiment. In this case the aldehyde functional group that is present in the products (monosaccharides and some disaccharides) in this reaction is able to reduce copper in the presence of alkali and this produces colour changes while converting to an aldose sugar. Honey is made of fructose and glucose which instantly turned brown after the test-tube was placed in the boiling water because of its active aldehyde and carbonyl group. The copper (II) sulphate present in the Benedict’s solution reacts with electrons from the aldehyde group which results in a redox reaction to from cuprous oxide, a red brown precipitate that seen in all of the above mentioned solutions (Hill, 1982). Beer also gave positive results because it contains aldehydes and ketones (i.e. acetone, trans-2-butenal, furfual) during its beer production process where the sugars are converted through fermentation (Hill, 1982).
This is the second step. During fermentation, from a rough dense mass lacking extensibility and with poor gas holding properties, the dough slowly changes into a smooth, extensible dough with good gas holding properties. As the yeast cells grow, the gluten protein pieces clump together to form networks. Hence, the alcohol and carbon dioxide are formed from the breakdown of carbohydrates that are found naturally in the flour. Enzymes present in yeast and flour also help to speed up this reaction.
- Little to no oxygen will result in the E. coli cells producing large quantities of acetic acid, causing the growth medium to reach pH 4 or lower however, with proper aeration the cells will be able to use many organic acids as carbon sources and the pH of the growth medium will be maintained at neutral or basic ranges. Aeration is another important factor in determining E. coli cell growth however it can continue to grow in the absence of oxygen using fermentation and anaerobic respiration. - Optimal growth temperature is 37C, cannot grow well at temperatures higher than 42C and they can tolerate lower temperatures with lower growth rate. - The doubling time or generation time for most E. coli strain in a rich medium at 37C is 20 minutes.
For example, fermentation occurs in yeast in order to gain energy by transforming sugar into alcohol. Fermentation is also used by bacteria, they convert carbohydrates into lactic acid. Ethanol fermentation is done by yeast and certain bacteria, when pyruvate is separated into ethanol and carbon dioxide. Ethanol fermentation has a net chemical equation: C6H12O6 (glucose) > 2C2H5OH (ethanol) + 2CO2 (carbon dioxide). This process of ethanol fermentation is used in the making of wine, bread, and beer.
CONCEPT OF SHELF-LIFE EXTENSION: Chitosan is a polymer which is extracted from chitin. It is natural, and thusly biodegradable and biocompatible. As a novel food preservative, chitosan offers the possibility to form edible film coatings on fresh or processed perishable foods in order to extend their shelf life. The chitosan film has antimicrobiological properties which allow it to protect the food it is coating.
It is commonly known throughout human history that the energy used is burned from coal which creates biomass. During the Industrial Revolution, coal was an essential need to everyone, hence the discovery of oil as a substitute. Yet, the mass formation from the unearthing of oil causes more damage than benefits for the planet. Humanity had never seen a more compatible source in which came a higher demand for oil. As the public has urged to generate more oil, scientists theorized that fossil fuels will eventually run out, making way for a renewable energy route in the future (Mason).
What is fractional distillation? Fractional distillation is a method of separating miscible liquids using heat. This technique is used for the separation of liquids which dissolve in each other. Several simple distillations are completed during fractional distillation using only one apparatus.
“Biophilic design is the deliberate attempt to translate an understanding of biophilia into the design of the built environment” (Kellert, 2008). It involves building and landscape design that enhance human well-being by fostering positive connections between people and natural environment. It is an innovative design approach that aims to maintain, enhance and restore the benefits of experiencing nature in the built environment. It starts by observing the effect of the mutual interaction between the natural and the built environment. The learned knowledge is then reflected onto the design and construction of the built environment.
Review of Literature Environmental issues began to be discussed and debated only towards the end of the 20th century. Since then significant amount of literature has been penned down raising awareness about issues of pollution, deforestation, animal rights and several others however it has failed to result in major changes, ideas or even actions to save the environment. Several species of animals have become extinct; pollution level is at an all-time high, global warming is leading to severe climate changes all across the globe but these problems do not seem to alarm the decision makers. Leydier & Martin (2013) also states that, “despite the increasing expression of concern in political and media debates about issues such as climate change, pollution and threats to biodiversity, “political ecology” (operating at the confluence of scientific developments, political engagement and ethical debates) is still trying to find its bearings” (p.7). It is quite evident that environmental issues are not treated in equivalence to political, economic, social or even religious issues.
