In a non-reducing sugar 3cm cubed and 10 drops of hydrochloric acid is placed in a test tube for a water bath of 5 minutes to be mixing afterwards. Biurets reagent is added to the protein solution to determine it presence. Testing for
Starch amylase testing was equally unsubstantial since the only amylase producing bacteria was ruled out after Gram staining. Unknown #10’s negative citrate test result was also unhelpful because E. coli is citrate negative and P. vulgaris is a variable citrate producer that can also be citrate negative. H2S production in the Kligler’s Iron Agar test ultimately proved that Unknown #10 was Proteus vulgaris. P. vulgaris is the only assigned bacteria that produces H2S, so when a black precipitate obscured the yellow butt of the Kligler’s Iron Agar slant, E. coli was ruled out. Not only did the H2S product confirmed that Unknown #10 was P. vulgaris, it confirmed P. vulgaris’ motility.
The purpose of this lab report is to employ a myriad of skills, tools and, methods learned throughout this semester to perform the appropriate tests for the identification of the assigned unknown bacteria. Add more background information here!!! The most important tools and techniques used during this identification include aseptic technique, microscopic examination and, the use of selective and differential media. Aseptic technique is an important tool for microbiologists. It is imperative that aseptic technique is maintained throughout the length of any test to avoid any cross-contamination that may lead to inaccurate results.
The Melibiose (MEL), Arabinose ARA, nitrate reduction, and catalase tests were all positive, and the oxidase test was
DIY - What Is Life? How can you determine whether something is alive, dead, or non-living? Whenever we speak of life, we must think in terms of cells.
Crystal violet was then added for 60 seconds before being washed off with water. The mordant, Gram’s Iodine, was added for another 60 seconds before getting washed off with water. The heat fixed smear was then washed with 95% alcohol until the wash ran clear, leading to the final step of adding Safranin for 45 seconds before being rinsed with water. The slide was finally blot dyed with bibulous paper before it was placed under a microscope to observe the color and shape of the bacterium. 2.2 Litmus Milk Reaction
Staphylococcus epidermidis is the organism that was identified based on the tests that I had conducted. The tests that I used to identify this organism were the coagulase test and the catalase test. My bacterium was beta hemolytic as well. First, a gram stain had to be done to determine whether the organism was a gram positive organism or a gram negative organism. This determined which set of tests that had to be done.
Catalase activity test establishes whether the bacterium produces the enzyme catalase. The eosin methylene blue test or EMB, inhibits the growth of gram positive bacteria and tests whether or not gram negative bacteria can ferment lactose. Lactose fermentation testing is done to see if the bacterium is capable of fermenting sugar by testing for acid and gas production. These are the possible tests that are needed in order to identify unknown
Molecular analysis is a well-known method and recently used by researchers. Using this
In this lab, genes for a fluorescent green protein (GFP) and antibacterial resistance (ARG) were inserted into E. coli bacteria. E. coli bacteria was resuspended in an ice-cold CaCl2 solution. DNA containing GFP and ARG was added to half of the cells before they were “heat shocked” in an ice bath and hot water. The heat shocking made the bacteria’s cell membrane more porous, so the DNA could enter. Recovery broth was added to the cell suspension, and the bacteria was placed in warm water for about thirty minutes (see Results and Discussion, paragraph 2).
Joshua Miller 12/18/17 Fermentation Lab report Introduction The term fermentation refers to the chemical breakdown of a substance by bacteria, yeasts, or other microorganisms, typically involving effervescence and the giving off of heat (wikipedia). Sugars are converted to ethyl alcohol when fermentation happens. In this experiment we determined if yeast cells undergo fermentation when placed in a closed flask with no oxygen. Glucose and yeast are mixed together in a closed flask and allowed to incubate for about one hour.
The tests performed to determine these characteristics are: a Feulgen Stain test to determine if the sample contains DNA or not, a Fat test to determine the absence or presence of fats and oils, an Iodine test to determine the absence or presence of starch, a Biuret test to determine whether the sample contains protein or not, a Benedict’s test to determine whether the sample contains a low, high or no amount of reducing sugars, and finally a Tetrazolium test to determine whether the sample is alive or
Finally, the amplified DNA regions are compare using a gel. DNA Profiling
This can be tested by simply mixing the serum of suspected individual which contain the antibodies with the antigens of specific bacteria the accumulation of clumps confirms the presence of particular bacterial infection.[2] This test can be performed in various ways including slide agglutination reaction, tube agglutination reaction, indirect agglutination inhibition reactions etc. Another important practical application involves blood group test of