Nt1310 Unit 1 Lab Report

LABORATORY REPORT EXERCISE #5

INTRODUCTION TO THE COMPOUND LIGHT MICROSCOPE, PLANT AND ANIMAL CELLS

Name_______________________________Section_____Teacher______________Date________

PRE-LAB QUESTIONS - answer the following questions using your textbook and valid internet sources. Be sure to cite your sources at the end of the prelab. You can type your answers to all questions except #1 and #9 directly into this document and then submit via Canvas. Type the answers for #1 and #9 at the end of the document.

1. Read and complete [Lab 5 Microscope Workbook Pages.pdf].
2. Identify the parts of the microscopes below.

3. Watch https://www.youtube.com/watch?v=SUo2fHZaZCU to understand how to properly use a microscope. Also watch https://www.youtube.com/watch?v=qSsMe_OXv-0

Describe the main difference between a compound light microscope and a dissecting scope. The main difference between a compound light microscope and a dissecting scope is that a light microscope has a higher resolution and a dissecting scope displays the image in a 3D form.
6. a. Distinguish between magnification and resolution? The difference between magnification and resolution is that magnification refers to how large an object appears compared to its actual size, while resolution describes how clear the specimen can be viewed in the microscope.
b. Explain the benefits of a higher resolution. The benefits of a higher resolution are that a specimen can be seen more clearly and the details of the specimen will be more obvious.
7. Explain how you will adjust the light on the compound light microscope. To adjust the light on the compound light microscope is to focus the condenser and adjust the diaphragm of a microscope until an individual sees the specimen. Also, an individual can adjust the light intensity to select the proper lighting they need.
8. What is Lugol's Solution and why can it be used for microscopic staining? The Lugol’s Solution is a water solution containing iodine and potassium iodine that suppresses thyroid function. It can be used for microscope staining because the solution turns black when interacted with starch. This can make the cell’s organelles more

There may be a quiz on procedures for lab, how to properly handle a microscope, and how to use its parts.

9.
1. Adjust the distance between the eyepieces so that they are comfortable for your eyes.
2. Focus the eyepieces to adjust your view.
3. Adjust the illumination to an appropriate level by adjusting the iris diaphragm and the condenser. The light should appear on the side directly below the objective lens, and give an even amount of illumination.
4. Rotate the objective lens until the shortest lens is in place (pointing down towards the stage). This is the highest power objective lens.
5. Turn on light source.
6. Place the slide on the microscope stage. Secure with the sample clips.
7. Focus and centre the specimen using the high objective lens. Adjust focus using the fine focus knob only.
8. Focus and centre the specimen using the medium objective lens. Focus firstly with the coarse focus knob, then with the fine focus knob (if needed).
9. Focus and centre the specimen using the low objective lens. Focus firstly with the coarse focus knob, then with the fine focus knob.
10. Fine tune the illumination so you can view maximum detail of your