ipl-logo

Paper Chromatography Essay

841 Words4 Pages

Complex mixtures can be separated and analyze using physical methods. One of it is chromatography. Two components in a mixture are separated by using the different distribution between two non- miscible phases which is stationary phase and mobile phase. The stationary phase exists as liquid or solid and it is fixed in a system. The mobile phase is a fluid which streams through the chromatographic system. In general, the process of chromatography is about a mixture of various components enter the chromatographic process and different components are flushed in different rates through the system. The mixture migrates at different rates over adsorptive materials during the separation. In short, the principle of chromatographic separation describes …show more content…

Paper chromatography experiment was done by drawing dots on the pencil line using magic pen of three different colours (red, blue and green). The chromatography paper was then placed into the beaker which contains acetone. For thin layer chromatography (TLC), flowers were used in this experiment. Paste-like form of flowers was extracted by grounding the flowers in a mortar and pestle. By using toothpick, spotting points were spotted on TLC plate. TLC plate was put into the beaker with spots towards the bottom of the …show more content…

The different components of the ink mixtures travel accordingly to different rates. The mixtures are then separated into different coloured spots depending on the compound's preference. The mobile phase is solvent in thin and paper chromatography.. Acetone is generally used as dipping reagent in chromatographic process because it shows the characteristics of a good solvent since it is important to choose a solvent for the color reagent in which the substances to be detected are insoluble (Joseph Sherma, 2013). Capillary action causes the solvent to flow up the paper at a uniform rate. A line called as solvent front is created across the paper .As the solvent level increased; the solvent dissolved the ink into its components. The rate of dissolving in the solvent differs because of the different markers which contains different components. The farther the ink travels upwards, the more it is attracted to the

Show More
Related
563 Words | 3 Pages

This addition aids in controlling the reproducibility and retention. Separation of the mixture via RP-HPLC can be done using continuous gradient or stepwise to move out the sample components. For every separation, the ideal gradient and volume must be

Read More
728 Words | 3 Pages

The lab started off by measuring critical materials for the lab: the mass of an an empty 100 mL beaker, mass of beaker and copper chloride together(52.30 g), and the mass of three iron nails(2.73 g). The goal of this experiment is to determine the number of moles of copper and iron that would be produced in the reaction of iron and copper(II) chloride, the ratio of moles of iron to moles of copper, and the percent yield of copper produced. 2.00 grams of copper(II) chloride was added in the beaker to mix with 15 mL of distilled water. Then, three dry nails are placed in the copper(II) chloride solution for approximately 25 minutes. The three nails have to be scraped clean by sandpaper to make the surface of the nail shiny; if the nails are not clean, then some unknown substances might accidentally mix into the reaction and cause variations of the result.

Read More
1286 Words | 6 Pages

Filtering, evaporating, centrifuging, and decanting something will only physically change it. Chromatography is used to separate different parts of a solution so that it can be identified. It can work because different substances have different attractions to things. Distillation can separate substances, such as salt water, as long as it has different boiling points. It can even be used to purify salt water but it is not cost efficient or energy efficient so it is not suitable for everyday use.

Read More
469 Words | 2 Pages

More specifically, this lab was met in terms of gaining an understanding in separating an acid, base and neutral compound from a mixture and identify through melting point. Overall, the experiment was successful as the acid (benzoic), base (5-chloro-2- methoxyaniline) and neutral (biphenyl) compounds were correctly identified. The separation of mixtures compounds to give pure components is of great importance in chemistry and in specific in organic chemistry. Many synthetic reactions give mixtures of products and it is important to isolate the wanted compound with a precise methodology of extraction and purification. Identification of the compound can always be identified by melting point

Read More
615 Words | 3 Pages

When we collected liquid from our distillation separation method, liquids #5 and #4 came out clear (without the food coloring). We believe solid #6 is what made our sludge purple. The density of food coloring is the same as water: 1 gram per square millimeter, our density was very close to this it was 0.51g/ cm3 and we could have made mistakes when reading the graduated cylinder.

Read More
305 Words | 2 Pages

( once again the watercolor paper is attached just to the wooden palette.) On top of the watercolor paper there is a rod ( stick ) that is connected to the wooden palette. So it looks like a half a square. On the rod there is various holes on each side so each time you come down to a lower piece of paper

Read More
1167 Words | 5 Pages

Do the same for the other test tubes. Let the test tubes not be disturbed for about 3- 4 mins. Then add the Amylase solution to the Starch solution and start the stopwatch (immediately). After every 1 min take one drop from the test tubes and place then in the test plate that were

Read More
1486 Words | 6 Pages

Introduction The purpose of this lab is to use control variables to help identify different macromolecules. Biological systems are made up of these four major macromolecules: carbohydrates, lipids, proteins and nucleic acids. Carbohydrates are sugar molecules (monosaccharides, disaccharides, and polysaccharides) which make them the most abundant macromolecule on the earth. Lipids (oils and fats, phospholipids and steroids) are insoluble in water and perform many functions such as energy source, essential nutrients, hormones and insulators (Lehman, 1955).

Read More
1696 Words | 7 Pages

TLC profiling Ammonia in butanol was the appropriate solvent to use for the column chromatography of food dye. After testing for the appropriate solvent, the set- up for column chromatography was done (Figure 2.). With the use of a clamp, the column was clamped onto an iron stand. A small cotton ball was then pushed in the column until it reached the bottom by using a stirring rod.

Read More
1619 Words | 7 Pages

When run through a printing press, the paper is pushed into the grooves filled with ink and the image is

Read More
1545 Words | 7 Pages

INTRODUCTION A gas chromatograph (GC) can be utilized to analyze the contents of a sample quantitatively or in certain circumstances also qualitatively. In the case of preparative chromatography, a pure compound can be extracted from a mixture. The principle of gas chromatography can be explained as following: A micro syringe is used to inject a known volume of vaporous or liquid analyte into the head or entrance of a column whereby a stream of an inert gas acts a carrier (mobile phase). The column acts as a separator of individual or chemically similar components.

Read More
1458 Words | 6 Pages

The solution with the pigments was spotted 15 times on both region A and region B and then allowed to dry. When the plate was dry it was placed into the tank for at least 20

Read More
1182 Words | 5 Pages

Introduction Drug use in sports has always been a controversial issue. With athletes pushing for the top podium position, performance enhancing drugs can be extremely enticing. One of the main types of drugs used by athletes are stimulants such as cocaine, amphetamines or ecstasy. These can create unfair advantages in sports. To keep sports even and fair, certain drugs became prohibited.

Read More
767 Words | 4 Pages

That caused a new initial reading of NaOH on the burette (see Table1 & 2). The drops were caused because the burette was not tightened enough at the bottom to avoid it from being hard to release the basic solution for titrating the acid. The volume of the acid used for each titration was 25ml. The volume of the solution was then calculated by subtracting the initial volume from the final volume. We then calculated the average volume at each temperature.

Read More
967 Words | 4 Pages

DETERMINATION OF PERCENTAGE ETHANOL IN BEVERAGES 1. Introduction to Gas Chromatography Gas chromatography is a very powerful separation technique for compounds that are reasonably volatile. The components of a sample partitions into two phases, the 1st of these phases is a immobile bed with a great surface area, and the other is a gas phase that permeates through the immobile bed. The sample is evaporated and passed by the mobile gas phase or the carrier gas through the column. Samples separates into the stationary liquid phase, based on their solubilities at the given temperature.

Read More

More about Paper Chromatography Essay

Related Topics

Open Document