Salvadora Persica Disease

INTRODUCTION
Bacterial diseases is any type of disease caused by bacteria. Bacteria 's are a kind of microorganism, which are tiny forms of life that cannot see by easy only be seen with a microscope. The bacteria that cause disease are called pathogenic bacteria. Bacterial diseases happen when pathogenic bacteria inter the body and begin to reproduce and grow in tissues. Pathogenic bacteria may also emit toxins that damage the body. Common pathogenic bacteria and the types of bacterial diseases they cause include: Salmonella and Escherichia coli cause poisoning food . Staphylococcus aureus a different infections in the body canhappened, for example: boils, wound infections, abscesses, toxic shock syndrome, food poisoning and pneumonia.

persica Binomial name Salvadora persicL.Salvadora persica (Arak, Galenia asiatica, Miswak, Peelu, Pīlu, Salvadora persica, or toothbrush tree, mustard tree, mustard bramble), is a types of Salvadora.[15] Salvadora persica has antimicrobial properties. [16] Used for a considerable length of time as a characteristic toothbrush, its sinewy branches have been advanced by the World Health Organization for oral cleanliness utilize. Investigate recommends that it contains various medicinally valuable properties including abrasives, sterilizers, astringent, cleansers, chemical inhibitors, and fluoride.[17][18][19][20][21][22]Distribution]Native to: Algeria, Angola, Botswana, Cameroon, Chad, Egypt, Eritrea, Ethiopia, India, Iran, Israel, Jordan, Kenya, Libya, Malawi, Mali, Mauritania, Mozambique, Niger, Nigeria, Oman, Pakistan, Palestine, Saudi Arabia, Senegal, Somalia, South Africa, Sri Lanka, Sudan, Syria, Tanzania, Uganda, Yemen, Zambia, Zimbabwe[22] Also happens in Namibia.[23]Appearance] Leaves and blossoms in Krishna Wildlife Sanctuary, Andhra Pradesh, India. Salvadora persica is a little tree or bush with a warped trunk, from time to time more than one foot in width. Its bark is scabrous and split, whitish with pendulous limits. The root bark of the tree is like sand, and the internal surfaces are a significantly lighter shade of cocoa. It has a

And , it was washed .About 120g of dried leaves were obtained from fresh leaves weighing about 24g .And, they were powdered and extracted with 100 % Ethanol for about 48 hours. After extraction, the Ethanol extract was filtered by mesh cloth.
. 2-Preparation of nutrient agar plates:
Cup –plate method was used for screening the antibacterial activity of pure and dried 100 % ethanol extract .A commercial sample of amoxicillin was used as a standard and nutrient agar was used as culture medium. The natural agar consist of Yeast Extract, Tryptone , Lactose , Manniiol, Sodium Chloride , Dip otassium Hydrogen Phosphate ,Gelatin and Agar .Then , in a conical flask 14g of nutrient agar was mixed into 500 ml of distilled water and , The mixture was stirred and dissolved until most of the agar dissolve. By non-absorbent cotton wool plug the mouth of the flask was closed. By using the autoclave the agar was sterilized for 15 minutes.The cotton wool was removed. By the flame the mouth of the flask was heated before and after pouring the agar into the Petri dishes. And, the left hand the lid of the Petri dish was lift, just enough to enter the mouth of the flask and quickly was poured in agar (about 15 cm3). The neck of the container was heated again and the plug was replaced. The agar was poured in until base of Petri dish was covered.Then,the lid was replaced,