The purpose of this project is to further test the effect of pH levels, and how they affect the production of enzymes. The hypothesis for this experiment was the more basic the ph buffer the high the activity of the enzyme. The more acidic the pH buffer the less activity of the enzyme. The first time the experiment was done with only three pH levels that were tested. That helped find the range of pH levels that were needed to test the second time.
Discussion PV92 Gel Electrophoresis Results: Through the usage of gel electrophoresis the correct allele for each sample was able to be determined. Lanes one through three in the gel,were the positive control lanes they contained the PCR cocktail and a known high-quality template for the PCR reaction. First lane contained the sample with the +/+ allele, which had two copies of the ALU repeat allele. The first lane had a band at about 941 base pairs.
Equation 3.1 can be simplified to the following equation γ(t,m;m_m )= e^(α-βm)/〖(t+c)〗^p (3.2) Where a_0=a+bm_m , α=a_0 ln10 and β=b ln10 are defined. |γ_m (t,m;m_m )|=|∂γ(t,m;m_m )/∂m|=e^α/〖(t+c)〗^p βe^(-βm) (3.3) Where |γ_m (t,m;m_m )| represents the absolute value of the partial derivative of γ(t,m;m_m ), and it is the instantaneous daily rate density of aftershocks of magnitude m at time t following a main-shock of magnitude m_m. e^α/〖(t+c)〗^p denotes the mean instantaneous daily rate of aftershocks at time t following the main-shock of magnitude m_m. βe^(-βm) is the exponential probability density function of aftershock magnitudes.
I noticed that all 3 different substances provided had a different density. When I put about a ½ inch of the solution “H” it was very thick almost like a jelly consistency. In addition we added another unknown solution “M” into the same test tube that contained the substance “H” we observed that the substance slinked to the bottom of substance “H” and it started creating its own layer. The two layers of solution both did not mix together and was separated by some type of density suspension. Furthermore we introduced another unknown substance “G” This was the densest liquid sinking to the bottom.
Arsenate can replace inorganic phosphate in step 6 of glycolysis that produces 1,3-bisphosphoglycerate instead of glyceraldehyde 3-phospahte. This yields 1-arseno-3-phosphoglycerate instead, which is unstable and quickly hydrolyzes, forming the next intermediate in the pathway, 3-phosphoglycerate. This is the same product that is normally formed in step 7. This is a problem because the product forms before it should and therefore does not reach the enzyme so the energy released cannot be harvested to generated ATP. Arsenate wastes energy by the uncoupling phosphotransfer reaction so its very POISONOUS.
The pH scale is a scale that is used to read weather a substance is basic or acidic. It also has different levels of how basic or how acidic said substance can be. the scale has a range of numbers from zero to fourteen. Acidity goes from the numbers zero to six, with zero being the highest level of acidity and six being the lowest.
Part 2 of the laboratory hypothesizes that different PEG precursor combinations will have an effect on the modulus of the material due to the differences in mesh structure. Unlike part 1, part 2 focuses on variation in mesh structure due to the polymerization of 2, 4, and 8 arm PEGnorb with 2 and 4 arm PEG thiol, rather than the impact of polymerization time. Table II displays the precursor combinations, the resulting hydrogel modulus, and the mesh size of experimental groups 1-2, 4-5, and 7-8. Figure 2 (a) and (b) display the modulus of each group and the mesh size of select groups, respectively. Although trivial, the minimum modulus displayed in Figure 2 (a) is group 1, a combination of 2 arm PEGnorb, or PEG 2k, and PEGdithiol, or 2SH, of
This report is a response to your request on the latest progress of my research project for the GADPH cloning and sequencing on the plant mint. This report is about the rest of the experiment. There are two objectives for this lab, 1. To learn about the molecular biology techniques and bioinformatics analysis. 2.
This is because the amount of NaOH droplet can be altered when a burette is used thus, the NaOH solution can be dripped per drop at the time nearing the end point of the experiment as the colour changes rather quickly from light pink to pink. Conclusion: In conclusion, titration is an effective technique to determine the equivalence point, pH range, pKa, Ka and molecular mass of the unknown acid of the acid-base reaction. This technique is dependent on the colour change of the indicator which makes it easier to determine the end point of the experiment.
Just a side not before naming the ph levels is that the
Introduction: For my final lab, I was given the task of producing the coordination complex Tris(Oxalato)Ferrate(III) Trihydrate using the following equation: FeCl3+3K2C2O4H2OK3Fe(C2O4)33H2O(g) +3KCl(aq) As a result, 4.105g of green crystal complex was produced and analyzed based on percent composition. To complete this analysis, four other experiments using titration, visible spectroscopy, ion sensitive electrodes, and dehydration and were used to determine the composition of oxalate, iron, potassium and water in the produced complex.
A pH indicator is a solution or mixture that changes colour in the presence of an acid or base. Red cabbage contains a specific pigment called flavin(science kiddo), in acidic conditions this pigment will turn a red colour and in basic conditions it will turn a green colour. From this it is noticeable that the colour change
The more basic cell components, such as hemoglobin and eosinophilic granules, are stained orange to pink and are called acidophilic because they stain with acidic dye. Some structures within the cells stain with both components, such as neutrophilic granules, whereas the azurophilic granules stain with methylene azure. (Patel
• pH 7 – This indicates that the soil is neutral and has a balanced pH level. • pH below 7 – This signifies that the soil has a high concentration of acidity. • pH above 7 – This shows that the soil has a high level of alkaline. However, you should also take into consideration that not all plants have the same pH requirement.
ACIDITY TEST INTRODUCTION: Acidity is the total amount of hydrogen ion present in the food sample with the expectation of those bound to alkaline ions. The hydrogen ion can be either attached to acids or in the form of free ions or anions. Titratable acidity is different than total acidity although at times both terms are used to mean the same thing total acidity is the total amount of organic acids in the food sample. This all acids (tartaric, oxalic acid, citric acid, sulfuric acid, lactic acid and acetic acid)
