Synthesis Of Spinach

The data collected seems to support the hypothesis that in increased temperatures, more proteins in the spinach cell’s phospholipid bilayer would denature to allow more leaching of Mg2+ and Ca2+. There is a clear positive correlation between temperature and mass of Mg2+ and Ca2+. found in the heated water sample with spinach. Such is shown through the positive gradient of the regression line that has a high regression value, implying the data collected relatively closely follow the positive correlation.

The trend between increasing temperature and amount of leaching was rather unexpected since the rate at which Mg2+ and Ca2+ leached out of the spinach cells started to slow down at higher temperatures of approximately 45ºC and 50ºC. After

As a result, even in extreme temperatures, there wasn’t considerable change in amount of leaching because nearly all proteins were already damaged. In other words, all of the substances inside the spinach cells escaped outside because all proteins were damaged to create the maximum amount of ‘holes’ in the phospholipid bilayer. Since all of the substances in the cytoplasm were already out by a certain temperature, there would be little to no change if temperature increased further. This explains the plateau that starts to occur by 45ºC and

leached from spinach is totally accurate. In fact, the several random and systematic errors would suggest otherwise. One large source of systematic error was the surface area of the spinach used. Although the mass was equal, the surface area of the spinach used for different trials differed vastly. This would create inconsistencies amongst data sets. However, it is impossible to extrapolate how this would have affected the final data since it is difficult to extrapolate to what extent surface area influences amount of substances leached. Another large source of error is from not knowing that spinach also contained iron, a metal ion that also created a complex with EDTA. This would create a positive direction shift in the data since the calculated amount of Mg2+ and Ca2+ leached would be higher than expected because of the added iron. However, it is again very difficult to deduce to what extent iron affected the final data for each trial. Such errors lead to low confidence in the accuracy of