Tucson Water Research Paper

Tucson water
Tucson, Arizona is a city of 526,116 people and they are all supplied water from the same organization Tucson water. The water has restrictions on what contaminants can be inside the water, but what are the effects of the contaminants which they do not have restrictions on. If they have a mixture of Nitrate-N, Sodium, and Fluoride inside the water then it will cause harm to Caenorhabditis Elegans (C elegans) because the possible large amounts inside the water. C elegans are very similar to humans cellular wise and they are often being used in medical testing and research before moving onto larger subjects.Tucson water supplies a list of contaminants inside the water of tucson the goal of this research is to find if these amounts

Grow E Coli on an agar plate and grow about 3 of them. Take the e coli colony and swab it with a q tip once swabbed move the e coli to another plate by streaking them across another plate. Incubate the E Coli at 37 degrees celsius for 24 hours. Move the E Coli into the fridge to prevent overgrowth of the E Coli. Place C Elegans into the plates with the E Coli and leave for 24 hours. Examine the C Elegans to insure that the C Elegans have survived at the room temperature and continue to have multiple C Elegans surviving. Once this is done prepare the dilutions of all the subjects which we are testing. Start with 1% solution for Nitrate-N 100ml and move 10ml of the first well into the next. Fill the well with 90ml dh20 to reach 100ml. move 10ml of the second well to the third well. FIll the third with another 90ml dh20 to reach 100ml. Move 10ml of the 3rd well to the 4th well. Fill the fourth well with 90ml dh20 to reach 100ml.Move 10ml of the fourth well to the fifth well. FIll the fifth well with 90ml of dh20 to reach

Fill the well with 90ml dh20 to reach 100ml. move 10 ml of the second well to the third well. FIll the third with another 90ml dh20 to reach 100ml. Move 10 ml of the third well to the fourth well. Fill the fourth well with 90ml dh20 to reach 100ml.Move 10 ml of the fourth well to the fifth well. FIll the fifth well with 90 ml of dh20 to reach 100ml. Start with 1% solution for Fluoride 100 ml and move 10ml of the first well into the next. Fill the well with 90ml dh20 to reach 100ml. move 10 ml of the second well to the third well. FIll the third with another 90ml dh20 to reach 100ml. Move 10 ml of the third well to the fourth well. Fill the fourth well with 90ml dh20 to reach 100ml.Move 10 ml of the fourth well to the fifth well. FIll the fifth well with 90 ml of dh20 to reach 100ml.
Place the C Elegans into these plates with the diluted amounts of the contaminants and observe the amounts surviving in the amounts. by using multiple pictures using a microscope to see the amounts moving etc. Observe the amounts living and record the data to announce the