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Unknown Bacteria Number 5 Lab Report

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Being able to determining the identity of a bacterium is essential as treatments for different types of bacteria vary greatly. Without correct and timely identification of a bacterium, a patient’s condition is likely to worsen and the probability of death rises due to inaccurate treatment. The identification of bacteria is also crucial in the prevention of bacterial diseases. Identification of bacteria also aids the selection of antibiotics used against them. This assists in the reduction of the overuse and misuse of antibiotics which aids bacterial resistance to antibiotics. The identification of bacteria aids in the detection of a bacterium’s origin and methods of transmission. Knowing the origin and method of transmission of a bacterium enables us to prevent or reduce infection by the bacterium (Tortora). I have been assigned unknown bacterium number 5. In the following paragraphs I will detail the methods used in the identification of unknown bacterium number 5. The initial test conducted after being given unknown bacteria number 5 was a Three-Step gram stain. This test was conducted utilizing aseptic technique. I first aseptically prepared a smear using unknown bacteria number 5 and heat fixed the smear. I then flooded the smear with …show more content…

This test was conducted utilizing aseptic technique. I first properly labeled the test tube and aseptically inoculated an MR-VP broth tube with unknown bacteria number 5 using a sterile inoculating loop. After 4 days of incubation, I added 4 drops of methyl red ph indicator to the tube. The contents of the test tube turned yellow in color. This indicates a negative test result because unknown bacteria 5 utilized the butanediol pathway instead of the mixed acid fermentation pathway. The negative result of the Methyl red test eliminated approximately half of the remaining genera in the family Enterobacteriaceae in the 9th Edition of “Bergey’s Manual of Determinative

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The six bacteria used in this lab were, Citrobacter freundii, Bacillus subtillis, Enterobacter aerogenes, Staphylococcus epidermis, Enterococcus durans, and Escherichia coli. Citrobacter freundii is a Gram-negative rod shape bacteria. The MSA plate will grow Citrobacter freundii, Bacillus subtillis, Enterobacter aerogenes and will have a yellow color change while Staphylococcus epidermis will not grow nor have a color change to yellow. The MacConkey agar will have growth with Escherichia coli, Enterococcus durans, but not Staphylococcus epidermis and Bacillus subtilis since it is a Gram-positive.

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Staphylococcus epidermidis is the organism that was identified based on the tests that I had conducted. The tests that I used to identify this organism were the coagulase test and the catalase test. My bacterium was beta hemolytic as well. First, a gram stain had to be done to determine whether the organism was a gram positive organism or a gram negative organism. This determined which set of tests that had to be done.

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