Practical 2
Title: Ion selective electrodes (ISE): pH meter, glucometer
Aim:
1. To introduce the use of Ion selective electrodes (ISE): pH meter, glucometer
2. To learn the working principle of Ion selective electrodes (ISE): pH meter, glucometer
Introduction:
Electrolytes are positively and negatively charged molecules and are measured using the method known as potentiometry. It will measure the voltage that develops within the inner and outer surfaces of an ion selective electrode. The electrode membrane is created by a material that is selectively permeable to the ion. Its measure the potential by comparing it to the potential of a reference electrode.
Electrolytes with a positive charge are magnesium, sodium, calcium, and potassium. The negative ions include bicarbonate, phosphate, and chloride. The concentrations of those ions in the bloodstream remain consistent during the day in a healthy person. The changes in the concentration of one or more of those ions can happen through several severe and chronic disease states and can lead to serious outcomes.
The experiment principle is involving the ion-selective electrode (ISE) and also known as a specific ion electrode(SIE). It is a sensor that converts the activity of a specific ion dissolved in a solution into an electrical potential. The voltage is theoretically subject on the logarithm of the ionic activity. In this experiment, it includes two apparatus which are glucometer and pH meter. pH meter recognizes