Agar plate Essays

Bacillus thuringiensis was isolated from raw milk (Taif, KSA) on nutrient agar at 37oC for overnight. The supernatant of the bacterial isolate was screened for synthesis of AgNPs. The bacterial isolate was morphologically and biochemically characterized according to Bergy’s Manual of Systemic Bacteriology[21]. Also, this bacterial isolate was further identified by 16S rRNA sequencing. The culture was maintained on a nutrient agar plate/slants at 4°C and as glycerol stocks 40% at −70°C. Preparation of bacterial

The creation of the master plate was made up of specially selected bacteria from both experiment 3.2 and 4.1, the goal was to achieve a diverse selection of bacteria to observe closely at its texture, color, shaper, and margins. When selecting specific bacteria, I looked for the best combination of variation and choosing across all five of my plates for both agars. Through using morphological observation I was able to determine different bacteria such as colonies that are irregular and circular continuing

Streak plate The streak plate was successful due to the growth of colonies in the media. The unknown microbe was separated in small individual colonies. The colonies are light yellow in color, irregular shape, and shiny in texture. The successful separation of individual colonies is the foundation for many tests that will be used for the identification of the unknown bacteria. Gram Stain Gram stain is a common technique used to distinguish between gram positive and gram negative. Gram positive

the unknown bacteria we had to use the streak plate technique to isolate separate colonies. We used a nutrient and MacConkey agar plate to begin. We used both agars because the nutrient agar allows us to grow the largest number of different types of microbes, yet not all bacteria can grow because it is rich with beef broth and some yeast extracts. McConkey agar only allows gram negative bacteria to grow while inhibiting gram positive bacteria. This agar also contains a pH indicator which allows us

.4 Agar Disk Diffusion The antimicrobial tests were carried out according to disc diffusion tests (Lennette et al., 1985, Kim et al., 1995). Cells were streaked on MHA agar plate to obtain colonies at 37°C for 16 hrs after which they were resuspended in sterile saline solution to give an O.D of 0.1 at 600nm. On solidified 2% MHA plate, the bacterial culture is swabbed and sterile disc impression was made, 10 µl plant extracts was loaded on the agar plate. After 10 minutes the plate is incubated

We took 2 Petri plates containing L-agar and spread 50µl from dilutions 〖10〗^(-3) and 〖10〗^(-5) respectively on L-agar plates by using spreader and incubated them at 37°C for 24 hours. After 24 hours incubation, we selected isolated colony and streak it on L-agar containingPetri plates to purify it. After 24 hours incubation, we got our purified colony, now we checked either it is nitrogen fixing

petri dishes were obtained air and skin. The air petri dish lid was left open so airborne bacteria could land in the petri dish. The skin petri dish was divided into four sections for each lab member. We each pressed our fingerprints into the correct agar section to capture bacteria from our skin (Holbrook and Leicht, 2017).

were added to 1 ml of MHII containing different concentration of ciprofloxacin 0.5x, 1x, 5x, 10x, 20x, 30x, 50x,75x and 100x MIC to each well in 12-well plate. Colony counts (CFU/ml) were determined at different time points (T0, T3, T24) by using appropriate dilutions of each culture. Using spot-plating method (32) 10µl was spotted on LB agar plates. However after 24 hour exposure the bacterial cell from dilutions of ≤ 1x102 were washed twice with sterile PBS prior to plating in triplicate. These

Chemistry Exploration Topic: determining the activation energy of a chemical reaction Research Question: What effect does temperature of the chemical reaction have on the activation energy ? ICT: Microsoft Word Autograph Microsoft Excel Introduction This experiment is designed to help in estimating the activation energy of the rate-limiting step in the acid catalyzed reaction of acetone with iodine. This is achieved by measuring the reaction rates at different reaction temperatures over

Amoxicillin tubes showed growth in all 9 tubes and the control tube. Thus, MIC could not be determined for Amoxicillin. After the incubation period of the six plates prepared from using the clear tubes (Vancomycin), the results were obtained as below, Plate 1 Plate 2 Plate 3 (100 ug/ml) (50 ug/ml) (25

phenospecies test (morphology and biochemistry), based on the protocol of SNI 7303 (2009), plus one control isolate the A. hydrophila ATCC 7699 obtained from Microbiologic Co. Figure 1. The Isolate of A. hydrophila ATCC 7699 Grown on the Rimler-Shoots Agar Medium + Antibiotic Novobiocin at 37C for 24 Hours 3.2 The

Materials and Methods The animal experimental procedure was approved by Ethical committee of ICAR-National Institute of Animal Nutrition and Physiology, Bangalore, India. Incubation and in ovo treatment A total of 200 uniform sized eggs (Cobb broiler) were procured from commercial hatchery and incubated with the dry bulb temperature ranging from 99 - 100°F and wet bulb temperature of 85-87°F from day 1 to 18. On day 18, all infertile eggs were removed and the fertile eggs were divided into 3

E. Discussion: In order to synthesize the polymer, Nylon 6,10, we had to complete a few steps to create the chemical reaction that combined sebacoyl chloride and hexamethylenediamine. First we measured the mass of the two graduated cylinders when they were empty, and measured it again after they were filled with sebacoyl chloride and hexamethylenediamine. We did this in order to find the measurements of the reactants. When we measured the graduated cylinder when they were emptied, one weighed at

register, then going towards the kitchen door. There is another set of footprints starting by seat A going directly to the kitchen door. The cash register is open, and money is shown coming out. On the lunch counter, in front of seat A, there is a served plate with food on it, and a check paper by its left. To the right, there’s an empty seat without a label. Around the lunch counter are three seats, B, C, and D. All of them

Aesop’s Fables in The Modern World Aesop is the originator of this genre (fables); the Greek people chose the name and created a several legends about Aesop. In Norton Anthology of Western Literature book it says about him, “He had supposedly been a slave and was known for his ugliness and outspokenness. But there is no reason to believe that this has any historical accuracy. The stories about Aesop gave the Greeks and Romans a way of talking about the fables.” He gives a new way to represent ancient

The theory of plate tectonics was formulated in the 1960’s, to provide a realistic and complete idea of the processes that produce the Earth’s surface. These plates make up the Earth’s strong outer layer, the lithosphere. “This layer is about 100km thick, which includes the crust and the uppermost part of the mantle.” (Rafferty, 2010) Before the late 1960’s, geologists held the perspective that all the continents and ocean basins were in fixed positions. However this view was quickly dismissed, as

How the Science of earthquakes was started in North America after the California Earthquake of 1906 The California earthquake of April 18, 1906 is one of the most significant earth quakes of all time. The 7.9 magnitude earthquake ruptured the northernmost 477 kilometers of the San Andreas Fault from the northwest of San Juan Bautista to the triple Junction of Cape Mendocino. The earthquake caused severe damage with reports indicating that it caused more than 3,000 deaths and destroyed more than 28

The Tohoku Earthquake was the most powerful earthquake recorded to have hit Japan. The earthquake was a magnitude 9.0 off the coasts of Japan that occurred at 2:46pm on Friday 11 March 2011, which triggered a powerful tsunami that reached the height up to 10.4 meters. A Japanese National Police Agency reported 15,889 deaths, 6,152 injured, and 2,601 people missing, 127,290 buildings totally collapse, 272,788 buildings half collapse, and another 747,989 buildings partially damaged. The Tohoku earthquake

Aerial volcano lies at the convergent plate boundary between an oceanic and continental plate. It is believed to have been formed through the process of subduction. When the two plates converge, the denser and thinner oceanic plate gets bent, or subducted, beneath the lighter and thicker continental plate. The magma rises, becoming lava when it reaches the Earth’s surface. When the lava cools, it forms rock. Over time, after several eruptions in which magma explodes to the surface when pressure in

Speech More than 100,000 tons of gum is being consumed every year by people, and most of those people are students. If you ask anyone if they like gum they will most likely say yes and that is probably because in the modern world, we have incredibly diverse flavors of gum. People chew a lot of gum, but is it actually good? Or bad? Gum actually has a lot of benefits and I am only going to mention multiple out of many. FIrstly gum helps students stay focused in class and outside of class. It’s