Chromatography software Essays

Introduction: Benzopinacol can be prepared from benzophenone in presence of sun light (photochemically) using isopropanol as the reducing agent in presence of acetic acid. Acetic acid is added to prevent the cleavage of benzopinacol to benzophenone and benzhydrol by the alkali derived from the glass container used for the reaction. Benzopinacol obtained in the first experiment is converted to benzopinacolone by heating under reflux (5 minutes) with glacial acetic acid containing trace amount of iodine

TFCH2002 Organic Chemistry II Lab Report Experiment title: A Floral Fragrance, Methyl Benzoate Name: Conor Fitzpatrick Student number: C14740151 Course code: DT422/2 Report submission date: 02/03/16 Aims • To convert benzoic acid and produce methyl benzoate by Fischer esterification. • Learn reaction mechanism involved in esterification. Benzoic acid + methanol  Methyl benzoate + water Introduction The ester functional group can be synthesized a number of different

stirred for the appropriate reaction times at 80 °C (Table 2). After completion of the reaction (monitored by thin-layer chromatography, TLC), the mixture was diluted with Et2O and filtered. The organic layer was washed with 10% NaHCO3 solution and then dried over anhydrous Na2SO4. The solvent was evaporated under reduced pressure and the product purified by column chromatography on silica gel to give the corresponding pure aryl

Gellan gum is an anionic deacetylated exocellular polysaccharide secreted by Pseudomonas elodea with a tetrasaccharide repeating unit of one á-L-rhamnose, one â-D-glucuronic acid and two â-D-glucuronic acid residues 30. It has the propensity of gelation which is temperature reliant or cations induced .This gelation involves the formation of double helical junction zones followed by aggregation of the double helical segments to form a three-dimensional network by complexation with cations and hydrogen

Preparation of ketoconazole loaded Proliposomes Ketoconazole (KTZ) loaded proliposomal gel formulations were formulated by method reported by Perret et al. 1991 with slight modification. Constant amount of drug was added to varying ratios of phophatidylcholine and cholesterol which constitute lipid component of 1mmol quantity. This lipid mixture was prepared in clean and dry, wide mouthed glass vials to which 400µL of absolute alcohol was added and after confirming the formation of homogenous dispersion

affinity of the component towards the stationary phase. In general, the more polar component would have a stronger interaction with the stationary phase, and the less polar component would be eluted out first. From the observations of the column chromatography, yellow S2 collected is β-carotene, indicating that

Thin Layer Chromatography (TLC) Abstract This experiment uses the TLC chromatography technique to identify the presence of acetylsalicylic and Acetaminophen in analgesic drugs (Tylenol and Anacin). It was found that the Anacin and acetylsalicylic had very closer Rf values (0.8 and 0.79). The Tylenol and acetaminophen had closer Rf values (0.54 and 0.58). Hence, Acetylsalicylic acid and acetaminophen were present in Anacin and Tylenol tablets respectively. Introduction Chromatography is the technique

COMPARISON OF INTRAVENOUS DEXMEDETOMIDINE VERSUS ESMOLOL FOR ATTENUATION OF HEMODYNAMIC RESPONSE TO LARYNGOSCOPY AND ENDOTRACHEAL INTUBATION Abstract: Background: Esmolol has an established role in attenuation of hemodynamic response to laryngoscopy and endotracheal intubation. We studied the effect of Dexmedetomidine compared to that of esmolol in this study. Aim: To study the role of dexmedetomine in attenuation of hemodynamic response to laryngoscopy and oral endotracheal intubation

Liquid chromatography is first being discovered by a Russian botanist, Mikhail Tsvet. Tsvet had rose the idea of liquid chromatography when he tried to purify and separate the coloured plant pigments by using a liquid-adsorption column containing calcium carbonate in 1890s. He also applied his observations with filter paper extraction to the new techniques for analysis the components in the petroleum. The filter paper extraction is the precursor of paper chromatography. He also found that the polarities

CHAPTER III BIGINELLI REACTION INTRODUCTION Dihydropyrimidinones (DHPMs), commonly known as Biginelli compounds, have attained unprecedented attention due to its greater biological, pharmaceutical and therapeutic properties. In 1893, Pietro Biginelli reported the first synthesis of 3,4-dihydroprimidin-2(1H)ones (DHPM) by a very simple multi-component one-pot condensation reaction of an aromatic aldehyde, urea and ethyl acetoacetate in ethanolic solution1 (Scheme 1.1). This efficient approach to

As previously stated, the following are the calculated RF values for each plate, Plate 1: Acetaminophen; Acetaminophen (Isolated)- 4.0/6.9= 0.57, Acetaminophen (Pure)- 3.8/6.9=0.55, Excedrin- 4.2/6.9=0.61 Plate 2: Caffeine; Caffeine (Isolated)- 1.9/6.8=0.28, Caffeine (Pure)- 0.8/6.8=0.12, Excedrin-4.0/6.8=0.59 and Plate 3: Aspirin; Aspirin (Isolated)-6.2/7.5=0.83, Aspirin (Pure)-6.2/7.5=0.83, Excedrin-6.8/7.5=0.9. The yield of each isolated analgesic was not calculated. The Rf values of the isolated

Before Gel Electrophoresis, separation of small molecules was impossible. Today Gel Electrophoresis is the primary method of separating molecules. The ability to separate has greatly improved forensics. paternity/maternity tests, and many other useful tests. Prosecutors being able to prove that a crime was committed because of DNA instead of testimony has improved the criminal justice system greatly. Oliver Smithies developed Gel Electrophoresis in 1950. To separate molecules an electric current

chromatography is meant those process which allow the resolution of mixtures by effecting separation of some or all their components in concentrated zone on or in phase different from those in which they are originally present , irrespective of the nature of the force or forces causing the substances to remove from one phase to another . ’’ (Williams et al, 1952) In this chromatography consists in applying a small drop of solution that is containing the substances to be separated to a strip of filter

1 Chromatographic decontamination 1) ion trade Decontamination of steed Ig by particle trade strategies has been portrayed. These creators took after the technique for Ter Avest et.al. (1992) with minor adjustments, utilizing DE-52 cellulose or DEAE CL-6b. 1gram DE-52 cellulose in 6ml 0.01m phosphate cradle (PB) ph6.0 was included every ml of serum. The DEAE CL-6b gel was washed twice with 0.5m Hcl, twice with 0.5m Naoh and twice with PB ph6.0 before utilization. For DEAE 1ml gel every 1ml serum

at the reference level. One technical overview Knowing that the room ambient noise didn’t affect the results too much, it would be interesting to cross correlate the best acoustic dampener configuration – the Slits panel vs the control. Not having software to cross correlate in depth, a simple subtraction between the two responses will be done and then plotted: The average amplitude implies that there is an average of around -1dB per frequency over the spectrum comparing the two configurations

44 Several technique such as HPLC, supercritical fluid chromatography (SFC), and GC are used for separation of chiral compound. As a complimentary technique, capillary electrophoresis is also used for enantiomer separation.45 This technique has the advantage of high efficiency due to the plug-like flow profile which is created by the electroosmotic flow (EOF) and the low solvent and selector consumption.46 In CE, electrokinetic chromatography (EKC) is mostly used of chiral separation. In this technique

Leah Romero 10/30/2017 Conclusion Lab 3 Chem 102L In lab 3, fundamentals of chromatography, the purpose was to examine how components of mixtures can be separated by taking advantage of different in physical properties. A huge process in this lab was paper chromatography, which was used to isolate food dyes that are found in different drink mixes. The different chromatograms of FD&C dyes were compared to identify which dyes are present in each of the mixes. Chromatograms where made for the known

I. Attention: Imagine you have to go to McKinley because you accidentally ate spoiled food. You ask for penicillin, but doctor is unwilling to prescribe you the drug and tells you that you will recover after resting for a few days (AA). Most physicians refrain from prescribing drugs to patients because the overuse of drugs can reduce their effectiveness. Transition: However, farmers are ignoring this fact, and it is becoming a public health problem. II. Need: Farmers are giving an excessive amount

Experiment: Density of Liquids Analysis Questions Directions: Answer in complete sentences. In your lab notebook, histogram the class data for the densities of Liquid A and Liquid B. Differentiate the boxes for each liquid with different colors or shading, and indicate this with a Key. For your horizontal axis, please use an interval width of 0.02 g/cm³ and count by odd numbers (i.e., 0.67, 0.69, 0.71, etc.). Don’t forget to give your graph a title, and label both of your axes. Once you’ve created

Spinach Leaf Chromatography Lab was to determine how much chlorophyll a, b and carotenes and xanthophylls the Spinach leaf contained through measuring the distance traveled by the pigments. The hypothesis the group created was that a spinach leaf contained multiple pigments. The group after doing the chromatography of the spinach leaf then determined the rate or flow of migration using Rf(Distance pigment traveled/ distances solvent 2. Materials and Methods A 2x15 strip of chromatography paper was