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Capillary Electroosmotic Chromatography Lab Report

517 Words3 Pages

Quantification and separation of chiral compound are considerable appealing because of the difference in pharmacological and toxicological properties of enantiomers. Sometimes one of the enantiomers demonstrates the desirable effect while the other could be less active or inactive or even have adverse effects.44 Several technique such as HPLC, supercritical fluid chromatography (SFC), and GC are used for separation of chiral compound. As a complimentary technique, capillary electrophoresis is also used for enantiomer separation.45 This technique has the advantage of high efficiency due to the plug-like flow profile which is created by the electroosmotic flow (EOF) and the low solvent and selector consumption.46 In CE, electrokinetic chromatography (EKC) is mostly used of chiral separation. In this technique the chiral selector is not immobilized on a stationary phase surface, but it is added to the buffer solution and migrates with a different speed than the surrounding buffer solution. Enantiomers have same electrophoretic mobility CE, but when the chiral selector is added to the background electrolyte different mobilities are acquired because of the different …show more content…

They are cyclic oligosaccharides consisting of linked D-glucose molecules. The most common commercial CDs are α-CD molecules, β -CD, and ϒ -CD which are composed of 6, 7, and 8 glucose molecule, respectively. In most of the CE chiral separations, positively charged or negatively charge CDs are used48. In most cases in EKC, negatively or positively charged derivatives of CDs are added to background electrolyte. In this system, the CD will have a stronger interaction with one of the enantiomers and the combination of the charged CD and the analyte will have a higher or lower velocity than the buffer solution and therefore the separation of the enantiomers will

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