The TLC plate was used to show the process and length of the reaction between the chalcone and sodium borohydride. It can be observed that reaction has finished when there are no spots of reactant left on the plate. On the plates from this experiment, it can be seen that the reaction had not finished after 40 minutes. Thin Layer Chromatography can be used to identify different chemicals in compounds. Each chemical has its own unique Rf value which can be used for identification.3 UV light was used for visualisation because the reaction solution was clear and the movement up the TLC plate could not be seen with visual light. The solution was fluorescent which made it visible with UV. There is a difference of 0.11 between the two Rf values