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Thin Layer Chromatography Lab Report

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The molecular formula is a way to tell if a structure is polar or not. Thin layer chromatography was used to see the polarity of components in a mixture. The silica gel on the TLC plate and the mixture can tell the relative Rf or Rf of the different spots on the plate. On the TLC plate, the order was β-carotene, pheophytin a or b, chlorophyll a, chlorophyll b, and then lutein. The relative Rf for β-carotene was closer to one compared to the other pigments, so β-carotene was more polar then the rest of the pigments. On the hand, pheophytin a or b, chlorophyll a, chlorophyll b, and lutein were less polar. The observations of the four pigments in UV light showed which pigment had red in them. The β-carotene had no red in the sample. The chlorophyll

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