Substrate Concentration Lab Report
The 0.1% is the concentration amount. Just like temperature and pH, substrate concentration can speed the reaction only up to a certain limit. When we mixed pH 3 enzyme tube with substrate tube, we used 0.3 mL of hydrogen peroxide, but if we were to increase the amount, then the experiment would have been faster. Our
K.D.A. Saboia et al. , (2007) have been prepared the Bi4Ti3O12–CaCu3Ti4O12 {[BIT(X)–CCTO(100-X)]} composite powders through solid state reaction method and calcined in the range of 900 to 1020 ºC for 12 h. The as-prepared powders have modified in the form of thick film onto alumina ceramic substrate by utilizing screen printing. At 100 Hz, the value of dielectric constant (κ) of CCTO100 and BIT100 is 316.61 and 53.64 respectively. Conversely, the composite with X=20 % shows an unexpected dielectric constant of 409.71, which is around 20% higher in comparison with the CCTO.
LABORATORY REPORT Activity: Enzyme Activity Name: Natalie Banc Instructor: Elizabeth Kraske Date: 09.26.2016 Predictions 1. Sucrase will have the greatest activity at pH 6 2. Sucrase will have the greatest activity at 50 °C (122 °F) 3.
First one is the independent variable which was Magnesium chloride (Mgcl2) that we would be changing. The dependent variable was the speed of the Paramecium and to see how Magnesium chloride will change it. We had two treatment levels they were our control group without Magnesium chloride and experimental group with Magnesium chloride. Our experiment was replicated twenty times. In our control group sample size, we added drop of Paramecium on 217 ml dryl’s solution.
It was hypothesized that the optimal pH for the enzyme was pH 7 while the 1.0 ml peroxidase would have the best reaction rate. At the end of the experiment the results prove the hypothesis to be incorrect. INTRODUCTION Enzymes are proteins that allow a reaction to speed up. These proteins are made up of monomers known as amino acids.
By using a spectrophotometer to measure absorbance at 420 nm, the rate of enzyme activity after all reactions have come to a stop can be
For this lab the objective is to see how the properties of individual substances compare with properties of mixed substances. For this lab the research questions is mixture, homogenous mixture, and heterogeneous mixture. A mixture is a combination of 2 or more substances that are mixed together physically but not combined chemically. Also a homogeneous mixture is a uniform in structure or composition throughout the substance. Lastly, a heterogeneous mixture is a consisting of dissimilar parts of elements (not the same).
As a result of the pGlo gene being inserted into the nonpathogenic substance of E.Coli, this experiment is testing whether or not E.Coli will grow and be bioluminescent. The independent variable in this experiment would be the E.Coli because it is either going to reproduce or not reproduce. Also, the pGlo gene is an independent variable because as a result the E.Coli is either going to be glowing or not glowing. The dependent variable would be the number of bacterial growth with the 12-18 hour time
After an enzyme acts as a catalyst for a substrate it is free to be a catalyst for another substrate, it can infinitely be used as a
VARIABLES: There are independent variables, dependent variables, and controls. the one that is being tested and the one that is the inconsistent variable in the Independent variable like the volume and surface area of the agar cubes. The variable that is kept consistent is the dependent variable such as the percentage of diffusion of pigment in the agar cubes. The constant variable is the features of the experiment that is kept the same throughout the entire experiment such as, the amount of time the agar cubes are left in the beaker and the amount of acid in the beaker.
Controlled Concentration of amylase Amount of amylase/starch Ph of the amylase/starch The concentration of the Amylase was kept at 1% at at times throughout the experiment. 5cm3 of both will be used in each reaction. pH of the Amylase/starch will be kept the same.
ABSTRACT: The purpose of the experiments for week 5 and week 6 support each other in the further understanding of enzyme reactions. During week 5, the effects of a substrate and enzyme concentration on enzyme reaction rate was observed. Week 6, the effects of temperature and inhibitor on a reaction rate were monitored. For testing the effects of concentrations, we needed to use the table that was used in week 3, Cells.
What will be the effects of varying temperatures of hydrogen peroxide on the speed of the chemical reaction that occurs by the catalase enzyme within hydrogen peroxide? If the temperature of the hydrogen peroxide is increased to 40° or more the catalase enzymes reaction speed (time it will take for the filter paper to float to the hydrogen peroxides surface in the test tube) will drastically decrease and ultimately the enzyme will become less effective. Independent.
Critical Micelle Concentration The CMC (Critical Micelle Concentration) is the amount of a surfactant molecule in a bulk stage, beyond which aggregates of surface active agents, so-called micelles. The CMC is a significant distinctive property of surfactants for its application. Generally molecules have two different constituents with differing attraction for the solutes. The component of the molecule that has an empathy for polar solutes, like water, is assumed to be hydrophilic.
Research Question: How does increasing the concentration (1%, 3%, 5%) of amylase from the pancreas affect the rate of the breakdown of starch solution, measured by the time taken for the starch to turn into simple sugars using a stopwatch (0.01s) Personal Engagement: The reason why I chose to do concentration was because I found it to be the most interesting out of all the options. I wanted to learn about how both enzyme and substrate concentrations affect the rate of reaction. Learning about enzyme concentration is not of any significance to me, and I chose it just out of curiosity. *Background Information: Enzymes act as catalysts to kickstart and speedup the rate of reaction without being used up.
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