ipl-logo

Amino Acids Lab Report

1410 Words6 Pages

Processing Raw Data Calculations The equationsRf(A) =LAL0 and Rf(A) =LBL0 were used to calculate the Rf values, A standing for the distance the green pigment traveled and B standing for the distance the yellow pigment traveled. For trial 1 of Nandina domestica, the L0 = 3.50 cm and the LA = 0.70 cm. Therefore, Rf(A) =LAL0= 0.70 cm3.50 cm= 0.200. A Rf value of 0.200 corresponds with the amino acid arginine. This was repeated for each trial of each leaf and the amino acids were identified. Retention factors and closest corresponding amino acids Rf (A) Nandina domestica Amino acid Buxus sempervirens Amino acid Stachys byzantina Amino acid Trial 1 0.200 arginine 0 none 0 none Trial 2 0.345 threonine 0.257 glycine 0 none Trial 3 0.186 arginine …show more content…

The Rf values of the other trials were not affected enough to change their corresponding amino acids. In order for this to be reduced, a more precise measuring device should be used. Systematic error seems to have played a large part in some of the outlying data points. For example, trial 1 of Buxus sempervirens and trials 2 and 3 of Stachys byzantina didn’t separate into any pigments. This could be due to the texture or the toughness of the leaf. The Buxus sempervirens leaves are very tough and were difficult to rub onto the chromatography paper, possibly causing there to be less available to separate. The Stachys byzantina leaves are very soft and delicate, but also dry. It was difficult to get much liquid to transfer to the paper, which makes it less likely to separate. It seems that the systematic error had a greater effect on the data than the random error. This occurred due to insufficient experience with this method of chromatography. If more trials were conducted, then this error could have been decreased. In addition, the amount of chromatography solvent in each test tube was not always equal, as the pipet was not consistent. Therefore, the amount of solvent traveling up the chromatography may not have been equal in each trial. Despite the errors, the amino acid that was most abundant was arginine, which agrees with what Kumar et al. (2016) …show more content…

Since all three trials for each leaf were done at the same time, there was no way to learn from the mistakes. With more trials, the problem with the transferring of pigment to the paper could have been analyzed and solved. This would have led to more accurate Rf values and subsequently, more accurate amino acids. Each group of trials should have taken about 10-15 minutes but after a few trials, the papers may have been removed from the test tubes early. This could have led to incorrect Rf values by altering the ratio of the distance that the solvent traveled and the distance the pigments travelled. The chromatography solvent may have created a few issues as well. In a first attempt at the experiment, none of the leaf pigments separated from the origin. The solvent that was used was from a bottle of reused chromatography solvent. Once the solvent was used straight from its original bottle, the pigments began to separate. In addition, the solvent was not always at equal levels as it was difficult to precisely measure with the pipet. The measuring tool used for this experiment was not the most precise instrument that could have been used. This may have led to some miscalculations, but as seen before, they were not

Show More
Related
2136 Words | 9 Pages

Additionally, in the initial attempt at the experiment, it was believed that cuvettes 2 and 3 needed to be put through the colorimeter before mixing together in the 4th cuvette, when in reality they did not need to be. A random error would be that there was a logger pro used the second day as there was no go link present as it had been the previous

Read More
775 Words | 4 Pages

The serial 2-fold dilution were done with a volumetric pipette, its pump, and 10 mL volumetric flasks. Eight different solutions were produced, half of which came from Red 40 and the other half, from Blue 1. These different concentrated solutions were placed in a 10 mL volumetric flask, each labelled with either R for Red 40

Read More
1183 Words | 5 Pages

The final mass could be far off due to the water and chunks of expanded gummy bears found in the beaker, leading to an inaccurate result. As well, for the sugar solution, the result could have been different if a more accurate measurement of the sugar needed was made. For the specific result, the hypothesis stated, the sugar solution needed to have an equal amount of sugar content to the gummy bear which did not occur. Ensuring that the beaker contained 10 grams of sugar was off, due to prerequisite calculations that lead to too much liquid in the beaker that needed to be removed. To be correct, the hypotheses that were wrong could

Read More
563 Words | 3 Pages

3. In this experiment, the percent yield was 90%. This number implies that there was little error in this experiment. However, this result could have been caused by certain external factors.

Read More
759 Words | 4 Pages

This could have impact different sized cubes to varying extents. Human error also occurred when the 3cm3 cube had to be repeated as it was initially incorrectly measured. This could have contributed to the results being less reliable as the concentration of phenolphthalein in the agar cube could have been different from that of the other cubes (Unknown, 2008). The method was quite successful and produced relatively accurate results. Next time, a substance such as hydrochloric acid could be used instead of sulphuric

Read More
1650 Words | 7 Pages

Also, if the grass length stayed consistent throughout the experiment, results would have been more

Read More
1237 Words | 5 Pages

The actual data is the result on our experiment vs theoretical, which is based on the calculations above. I have also learned to pay more attention to draining out all of the product completely before continuing to test the experiment, as any small drop of contaminant can veer our results into a different

Read More
1572 Words | 7 Pages

This was done to get more accurate results. The first time the experiment was conducted it was tested at three different time points, at zero minutes, fifteen minutes and

Read More
222 Words | 1 Pages

One big error was the inaccurate information. The error mainly had to deal with the type of rocks used in the experiment. The lava rocks did help but easily fractured into sediments. So it was difficult to gather accurate weight after the trial was done because all of the small sediments couldn’t be gathered and weighed. If this experiment is to be done again in the future, it would be best to change the type of rock that is used in the experiment.

Read More
1672 Words | 7 Pages

In addition, another systematic error could be the weighting scale not being properly calibrated, it can be seen if the 50g masses weren’t 50g then the test would not be reproducible and deemed unreliable. This can be fixed by comparing the weight shown on each weighting scales. Because of the fact, there was no true value given, it was difficult to assume the percent error thus how much systematic error this practical was affected

Read More
1617 Words | 7 Pages

Next, the amount of seedlings in the tray that expressed a green or white phenotype were both counted. The group observed that there were eleven total seedlings; eight of those seedlings were green and the three remaining were white. The next step in the experiment was to add up the class totals of green to albino seedlings, as shown in table 1. After determining a group phenotype ratio of 8:3 and a class ratio of 42:10, the group questioned what defect the white plants could have that might make the seedlings appear albino instead of green. The students concluded that a lack of chloroplast could explain why some of the seedlings were albino. The lab partners also decided that for a recessive, albino phenotype to be expressed, GG, Gg, and gg had to be the genotypes of the seedlings.

Read More
451 Words | 2 Pages

For example, in the response experiment, a yeast solution was prepared without sugar mistakenly and thus had to be prepared again. This suggests that other errors in preparation and measurement could have been encountered. For the future, careful measurements using clean uncontaminated flasks would eliminate possibilities of such error. A source of error for the metabolism experiment involves the yeast’s yellow hue. It is possible that the color of the yeast caused the solution to look more

Read More
1046 Words | 5 Pages

The 3 concentrations of enzymes were 0.5 ml, 1.0 ml, and 2.0 ml of turnip extract, while the substrate consisted of 0.1ml, 0.2 ml, and 0.4 ml of hydrogen peroxide. In a separate tube, the control was made up of turnip extract and guaiacol, known as the color reagent. This was recorded the absorbance every 20 seconds for 3 minutes.

Read More
864 Words | 4 Pages

Due to the unaccountability of the inconsistency in droplet size, many of the numbers may be varied because in one trial a huge droplet may count as one, but in another trial, I may have counted a small droplet as one, which causes results to possibly be

Read More
1458 Words | 6 Pages

Introduction The term chromatography actually means colour writing, and signifies a technique by which the substance to be examined is placed in a vertical glass tube containing an adsorbent, the different segments of the substance traveling through the adsorbent at distinctive rates of velocity, according to their degree of attraction to it, and producing bands of colour at different levels of the adsorption column. The substances least absorbed emerge earliest; those more strongly absorbed emerge later. (Wixom et al., 2011) In chromatography of all types, there is a mobile phase and a stationary phase.

Read More

More about Amino Acids Lab Report

Open Document