Thin layer chromatography Essays

In this experiment, thin layer chromatography will be used to identify an unknown proprietary drug. From the results, the conclusion can be made that the Unknown drug given was Excedrin. Excedrin contains acetaminophen, caffeine, and aspirin and from our results these three compounds

Introduction The purpose of this experiment was to identify the composition of over-the-counter analgesics by the method of Thin Layer Chromatography (TLC). The TLC method is used for rapid qualitative analysis of mixtures to determine and identify its components and purity. A development solvent was used to separate the analgesics found in both the known drug sample and an unknown sample of over-the-counter medications. The pertinent techniques for this experiment are spotting the stationary phase

Thin Layer Chromatography Lab Report Miranda Tyree Lab Partner: Caitlin Price TA: Yi Zhou Lab Time: Thursday, 12 pm Lab Room: 207 Date: 09/23/2016 Abstract Experiments were performed to determine the active chemical ingredients in Tylenol and Anacin. The active chemical ingredients were determined by performing thin layer chromatography of four substances: Tylenol, Anacin, acetaminophen, and acetylsalicylic acid. The identity of the ingredients was then confirmed by using the co-spotting thin

Utilizing the techniques of acid-base extraction and Thin Layer Chromatography we were able to extract the three active components of Excedrin and confirm the identity of each compound. The three active components of Excedrin- aspirin, acetaminophen, and caffeine- all have varying acidities and polarity which allowed for the successful usage of acid-base extraction and TLC respectively. Acid-base extraction was applied by adding our compound to a basic solvent in order to isolate the components by

This Layer Chromatograph The objective of the lab was to reveal the active components in commercial analgesic preparations using thin layer chromatography. The thin layer chromatography separation method is a liquid mobile phase that carries the components of the mixture over a solid stationary phase. The stationary phase contains polar hydroxide groups on the surface of the silica gel. A component is separated from the mixture in the mobile solvent when it interacts with the immobile silica gel

Thin Layer Chromatography (TLC) Abstract This experiment uses the TLC chromatography technique to identify the presence of acetylsalicylic and Acetaminophen in analgesic drugs (Tylenol and Anacin). It was found that the Anacin and acetylsalicylic had very closer Rf values (0.8 and 0.79). The Tylenol and acetaminophen had closer Rf values (0.54 and 0.58). Hence, Acetylsalicylic acid and acetaminophen were present in Anacin and Tylenol tablets respectively. Introduction Chromatography is the technique

methods used for the analysis of phospholipids. All systems of chromatography consist of a stationary and mobile phase. A monster placed on a stationary phase, i.e., a solid or a liquid, and the mobile phase, a gas or a liquid, is allowed by modifying the system. The components of the sample will be separated on the basis of their ranging physical and chemical properties, imparting different affinities for the two phases. Thin layer chromatography (TLC) was the first chromatographic method for assessing

under ultraviolet light or spraying the plate with a chemical reagent. The distance a spot travels up a thin-layer plate can be assigned a numerical value known as the Rf value. Theory: The technique of thin-layer chromatography (TLC) uses a solid stationary phase and a moving liquid phase to separate the constituent of a mixture. A thin-layer plate is prepared by coating a glass plate with a thin film of a granular material, usually silica gel or aluminium oxide. This granular material serves as the

Thin layer chromatography is performed by using a thin, uniform layer of silica gel and is commonly used to compare and separate most poisons, inks, and dyes. Many inks contain a colorful compound, most can be separated using TLC. The silica gel is the stationary phase. The stationary

triethylamine are used. Thin layer chromatography will be run at a specified time in the reaction, and the results will be examined both quantitatively(via ImageJ) and qualitatively to which reaction reacted more quickly. With this information, the stereochemistry of the alcohol can be deduced. Theory: The competing enantioselective

cyanobacteria mainly use chlorophyll (green) and phycocyanin (blue), it also contains carotene (orange) and xanthophylls (yellow). We deducted an experiment to investigate the photosynthetic pigments of cyanobacteria using the electrophoresis and thin layer chromatography (TLC) methods to divide and examine the pigments. The electrophoresis

NMR analysis. For the Melting Point Range Determination, samples of our re-crystallised p-aminophenol and paracetamol were placed into capillaries and into the melting point apparatus where data was recorded during the melt. Then, for the Thin Layer Chromatography, a TLC plate was prepared with four small dots loaded with p-aminophenol, our recrystallised p-aminophenol, our resultant paracetamol and commercial Panadol® respectively. When TLC was completed, it was transferred to a UV lamp box for analysis

Gurleen Bhangoo Robinjot Kaur CHEM 3301-02 Professor Jeffery Crisman 21 March 2023 Abstract: Chromatography is an analytical method that is used to separate a mixture of chemical compounds into their individual components, allowing the individual components to be thoroughly analyzed. In this experiment, thin-layer chromatography (TLC) is performed to identify an unknown drug, and then column chromatography is used to separate out the components of the drug. The given unknown will contain one of the

Ritchmond P. Togonon, Abiather L. Tsai, Jordan Martin G. Uy Department of Sports Science, College of Rehabilitation Sciences *philliptuazon14@gmail.com Abstract This experiment is about the separation of pigments in capsicum annuum using column chromatography. In this experiment pigments in capsicum annuum or red pepper were separated. Four different eluates with different colors were produced from the column by adding eluents through the column. Keywords: capsicum annuum, eluate, eluent, dichloromethane

TLC Analysis of Analgesic Drugs Introduction The purpose of this experiment was to use thin-layer chromatography (TLC) to determine the composition of various over-the-counter analgesics (acetaminophen, aspirin, caffeine and salicylamide). The methods necessary was thin-layer chromatography Experiment Scheme Prepared at least 12 capillary micropipets to spot the plates. Then obtain two (silica) TLC plates and handle them carefully or the adsorbent may flake off. Handled them only by the edges. Used

Yiming Zhang September 13, 2017 TLC Analysis of Analgesic Drugs Introduction The purpose of this experiment was to determine the composition of the four counter analgesics on the counter by a method which called thin-layer chromatography (TLC). According to the appearing of the distance they have traveled we can identify if the unknown mixture contained acetaminophen, aspirin, caffeine or salicylamide. Experimental scheme Acetaminophen(Ac) Aspirin(Asp) Caffeine(Cf)

After the assigned reaction was complete, samples of authentic cis-cyclohexene-1,2-diol, authentic trans-cyclohexene-1,2-diol, a 50:50 mix of the cis and trans cyclohexene-1,2-diols, and the product were each spotted on the Thin Layer Chromatography (TLC) plate. Then the TLC plate was placed inside a saturated beaker filled ethyl acetate in order to develop the plate. Once the solvent traveled up the solvent front, the plate was stained with anisaldehyde solution and then heated with a heat gun so

The purpose of this experiment was to determine the identity of isolated separated compounds using thin layer chromatography and column chromatography methods. In part A, four TLC were ran once each with ethyl acetate, hexane, toluene, DCM as solvent to separate a mixture. The stock solution is a mixture of benzophenone, biphenyl, and diphenyl methanol. The last trial include the comparison with the standard using the best solvent. It was predicted that toluene and hexane were the best solvent

Chromatography of Spinach Formal Discussion This lab involved the extraction of pigments from spinach leaves which were then analyzed using thin layer chromatography. The first step of this process was to grind up the leaves in order to extract the pigments. Hexanes facilitated this process and afterwards, the solution was dried over sodium sulfate to remove water. Next, the column was packed with a small piece of glass wool, followed by hexanes, and then packing sand. At this point, the column

In thin layer chromatography, the example is separated in the flexible stage dissolvable. The ensuing course of action is then spotted near the base of the rectangular sheet of glass or plastic secured with silica. The sheet is then submerged in a chamber containing a little measure of dissolvable, keeping the spots over the surface of the dissolvable. Once the sheet is set in the chamber, the dissolvable begins to increase on the sheet through thin movement. The covering on the sheet is the staionary