Separation Of Pigments In Capsicum Using Column Chromatography Lab Report
Phillip Anthony C. Tuazon*, John Ritchmond P. Togonon, Abiather L. Tsai, Jordan Martin G. Uy Department of Sports Science, College of Rehabilitation Sciences *philliptuazon14@gmail.com Abstract This experiment is about the separation of pigments in capsicum annuum using column chromatography. In this experiment pigments in capsicum annuum or red pepper were separated. Four different eluates with different colors were produced from the column by adding eluents through the column. Keywords: capsicum annuum, eluate, eluent, dichloromethane, hexane Introduction Red Pepper Capsicum annuum or commonly known as red pepper is widely cultivated and found in tropical deciduous forests. Capsicum annuum(red pepper) is composed of six carotenoids: capsanthin, β-carotene, violaxanthin, cryptoxanthin, capsorubin, and crtyocapsin. The colored components of capsicum annuum are capsanthin and …show more content…
To prepare the column for chromatographic separation, a 5.75 inch Pasteur pipette is required; plugged with glass wool (or cotton) at the bottom. The column was filled up to ½ of the height of the pipette, and then loaded with a thin layer of sand. The sample was loaded on top of the sand and loaded again with a thin layer of sand. Eluents were loaded one after the other: hexane; dichloromethane with hexane; dichloromethane; and dichloromethane with methanol. Elution was the main process used in this experiment; there are two types of elution isocratic and gradient. Gradient elution was used in this experiment. Elution is the process of extracting one material from another with the use of a solvent. The eluent is the liquid solvent and the eluate is the product coming out from the chromatograph. Colored eluates were coming out of the column and were collected in separate test
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