Elodea” was put into a tank labelled “chemically treated Elodea”. Three drops of phenolphthalein were added to each beaker and then all of them were placed on a sheet of white paper. 0.02N NaOH was slowly added to the “tap water with Elodea” beaker with a dropper that delivers 0.025 mL per drop. Each drop was counted and the solution was stirred thoroughly before adding the next drop. This was continued till a faint pink color appeared in the solution. The number of drops was noted. The procedure
Materials and Methods: To gather the needed data on the crayfish, samples were collected at Pearson’s Creek, a little outside Springfield. The spring was right off the road and located directly next to a little over pass. Sampling occurred early morning 0830-0930 on a cloudy day. The weather was a typical mild late summer morning with a rain shower that had passed through the evening before. Two sampling areas that were 10-meters long and the width of the stream were marked off using flags
The product obtained in this experiment was a slightly yellow tinted liquid. The percent yield was 28.99%. This is indicative of a successful product. This percentage indicates, not only was product formed, but the correct amount of anhydrous sodium sulfate was added, which removed the water molecules from the product. Another factor contributing to the percent yield is the amount of time the product was left under gentle air. A higher or lower percent yield would have suggested an incorrect amount
Protocol for Initial TLC: A TLC chamber and a TLC plate were obtained making sure to wear gloves and to not damage the silica coating on the TLC plate. A line was drawn about 1 cm above the bottom of the plate using a pencil. The TLC plate was spotted with the unknown by adding a small drop to the plate with a capillary tube and then allowing the solvent to evaporate. The TLC chamber was prepared by adding enough methylene chloride to cover the bottom of the jar to about 0.5 cm depth. A piece of
Name: Ashrith Epuri Lab Members: Ian, Averi, Serena Percent Yield Lab Objective: Determine the percent yield of NaCl in a chemical reaction. Materials: Evaporating dish Watch glass Digital balance Sodium bicarbonate Wire mesh Scoopula Plastic pipette Hydrochloric acid Hot plate Crucible tongs Methods: First, the mass of the empty, clean, and dry evaporating dish and watch glass was recorded using the digital balance, while the hot plate was set to 450 degrees Fahrenheit. 1 gram of sodium bicarbonate
unknown to different solutions. The amount of 1.0 g was given of the unknown element at the beginning of the experiment. _1.0 g of a compound was placed in 100 mL beaker, then the unknown element was added in the compound . _ The mass of the glassware was taken after and before the unknown and water poured into the beaker. _The unknown compound and 40.0 mL of water were placed in the beaker, then observation was done to test the unknown element. _ The color of the unknown changed to cloudy
The last step for our experiment was creating a filter to clean our water. First cut the bottom off of a plastic bottle off and take the lid off. Next put the filter over the lid of the plastic bottle and place the bottle with the filter side in the 100 mL beaker. Once the bottle is in, pour an inch of charcoal, then another inch of sand. Lastly pour an inch of small rocks into the bottle as the top layer and pour in the contaminated water. The procedure for this experiment is to first get your
Assay of ATP-sulfurylase activity For the determination of ATP-sulfurylase activity in plant 0.3g fresh samples (leaf and root) were ground with chilled mortar and pestle using ice cool buffer consisting of 10 mm Na2EDTA, 20 mm Tris–HCl (pH 8.0), 2 mm dithiothreitol and approximately 0.01 g ml-1 insoluble polyvinylpyrollidone, using 1 : 4 (w⁄ v) tissue to buffer ratio. The homogenate was strained through gauze and centrifuged at 20 000 g for 10 min at 4 °C. The supernatant was used for the in vitro
Experiment 1: Materials: • Alka-Seltzer tablets • Empty and clean water or soda bottles (12 oz to 24 oz) • Balloons • Water • Clock • Stove top Procedure: 1. Pour a sufficient amount of water (about 16 oz) into a small pot and place on the stove at high heat. 2. Watch the clock and after 30 seconds take the water off the heat. 3. Drop one Alka-Seltzer tablet into one empty water bottle. 4. Pour the hot water from the pot into the bottle. 5. Stretch the open end of the balloon around
Synthesis of nanoparticles 1mM aqueous, and ethanol solution of silver nitrate was prepared for synthesis for silver nanoparticles. 1ml of plant crude extracts was added upon the various concentrations of silver nitrate solution such as 1mM, 3mM and 5mM and abrupt color changes indicates silver synthesis. 4.8 Oxalic acid preparation: The 100 mg of oxalic acid was weighed and dissolved in 100 ml of double distilled water for chemical silver synthesis. 4.8.1 Oxalic acid silver synthesis: 1mM of ethanol
In this experiment, racemic 2-methylcyclohexanone was reduced using sodium borohydride as a nucleophile to give a diastereomeric mixture of cis and trans secondary alcohols. The products were analyzed for purity using IR spectroscopy and gas chromatography. 1.2 g of 2-methylcyclohexanone and 10 mL of methanol were combined in a flask and cooled in an ice bath. Two 100 mg portions of sodium borohydride were added to the flask and stirred. 5 mL of 3M sodium hydroxide, 5 mL of de-ionized water, and
Exercise 2 began with measuring the milliliters needed to fill a coffee mug and measuring the liters in a gallon. Then we went to the sink and filled a graduated cylinder with 70 ml of water and then placed a pencil in the water. Our lab partner, Temi had to use both of her hands to push the pencil in the water in order to completely submerge it, because it kept floating upward. After completing these steps, we then calculated and recorded the volume. Once we finished this, we then repeated steps
Table 1 mentions the Column volumes of Gel Filtration experiment: Void Volume (Vo), Elution Volume (Ve), Included Volume (Vi), Entire Volume (Vo + Vi), and Inert Volume (Vg). Void Volume (Vo) came out to be 1.25 ml. It was calculated by adding all the fractions that contained blue dextran. Blue Dextran was the biggest substance in size which made it impossible to fit in the available pore sizes of the beads. Void Volume represents the space between the beads in column. In addition, Elution Volume
Introduction 1.1 Aim: To determine the kinetic parameters, Vmax and Km, of the alkaline phosphatase enzyme through the determination of the optimum pH and temperature. 1.2 Theory and Principles (General Background): Enzymes are highly specific protein catalysts that are utilised in chemical reactions in biological systems.1 Enzymes, being catalysts, decrease the activation energy required to convert substrates to products. They do this by attaching to the substrate to form an intermediate; the
After pumping for awhile, you will probably be able to stockpile a large amount of breastmilk in your freezer to use while you are at work or away from your baby for the evening. When it 's time to use your breastmilk, it 's important to thaw it correctly in order to keep it safe and healthy for your little one. How to Safely Freeze Your Breast Milk After you are finished pumping, immediately pour your breast milk into a plastic storage bag or bottle. Leave about one inch of room at the top of
100 mg Drug was weighed as per the recommended dose of Diclofenac Sodium. Accurately weighed of amount non- pareil seeds were dried to remove moisture at 35oC. These non pareil seeds has been transfer to a coating pan, which has a bed temperature of 350 C. Isopropyl alcohol : water (70:30) with 5% PVP K-30 was sprayed with the help of spray gun till the bed become wet. Immediately drug powder mixed with the talc was applied to the wet bed of the pellets. Mixing was done with a great care
In order to proceed with the experiment certain materials were needed in order to be able to perform well. The materials needed were as follows, unknown liquid, unknown solid, water, and acetone. The first thing that had to be done was to take the mass of a 25 ml flask and stopper which we got a mass of 31.54g. After that we filled the flask plus stopper with H2O and measure a mass of 60.50g. After taking the mass of the water the water was disposed of in the sink. The flask had to be dried to continue
Lab 2 – Water Quality and Contamination Experiment 1: Effects of Groundwater Contamination Table 1: Water Observations (Smell, Color, Etc.) Beaker Observations 1 There was no smell 2 When I pour the oil in the water I noticed the oil started to bubble to the top. So I got to stir the oil that’s when there were smaller bubbles. As I smell the water there was no smell to the water. Putting the vinegar I didn’t smell anything but I did see the water was a change it was dark that’s when I added the
There are many techniques that may be utilized in order to prevent, detect, and deter shoplifting. Roberson and Birzer describe a few of these techniques in “Introduction to Private Security”. Considering shoplifting is a common problem among businesses, many companies utilize one or more of these techniques in order to deter and prevent loss. In today’s world, technology is becoming more and more advanced every day. In turn, prevention and detection techniques are becoming more advanced and effective
SDS-Polyacrylamide gels were prepared and the glass plates were washed with 70% ethanol and water. After drying the plates, water was used for test leakages. Two SDS-Polyacrylamide gels were prepared according to the following recipe. These all above components of the running gel were added in a 50 ml tube and solutions were mixed and pipetted into the prepared gel chambers. Glass plates were filled ¾ and the gel was covered with 100-500 µl Isopropanol in order to achieve an even surface. When the