Synthesis of nanoparticles
1mM aqueous, and ethanol solution of silver nitrate was prepared for synthesis for silver nanoparticles. 1ml of plant crude extracts was added upon the various concentrations of silver nitrate solution such as 1mM, 3mM and 5mM and abrupt color changes indicates silver synthesis.
4.8 Oxalic acid preparation:
The 100 mg of oxalic acid was weighed and dissolved in 100 ml of double distilled water for chemical silver synthesis.
4.8.1 Oxalic acid silver synthesis:
1mM of ethanol solution of silver nitrate was prepared for silver nanoparticles chemical synthesis and 1ml of oxalic acid silver responsible compound dissolved solution was added upon the various concentrations of silver nitrate solution such as 1mM, 3mM and 5mM and abrupt color changes indicates chemical silver synthesis.
4.9 Characterization of silver nanoparticles
4.9.1 UV- Visible spectra analysis
The reduction of metallic Ag+ ions was monitored by measuring the UV- Visible spectrum after about 16 hours of reaction. The UV beams emitted upon the samples and its absorbed values were detected by sensors eventually provides the wavelength from 200nm to 800nm in Ultra visible
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The paper disc (6mm diameter, whatman no.1 filter paper) containing various concentrations of samples 5-20ug/ml was chosen and placed aseptically on the agar surface with the help of a sterile forceps and paper discs were pressed slightly with the forceps to make complete contact with the surface of the medium. The plates were kept at room temperature for half an hour and subsequently incubated at 370C and observed for zone of inhibition after 24 hours. The inhibition zone around each disc was measured in millimeter. The results were recorded by measuring the zone of growth inhibition surrounding the