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Coagulase Test

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Coagulase test Coagulase is an enzyme that causes plasma to clot by activating prothrombin to form thrombin which then catalyzes the activation of fibrinogen to form fibrin. Coagulase test was performed one drop of citrated human plasma was added on a slide by using a plastic loop or wooden stick,. Mix well and clumping was observed within 10-15 seconds indicate the coagulase positive test. Citrate test- Fresh (16- to 18-hour) pure culture was used as inoculation source. A single isolated colony was slightly streak on the surface of the simmon citrate agar slant. Incubate at 35ºC for 18 to 48 hours. Growth was observed at the slant surface and the color of medium was changed from intense green to a deep Prussian blue. Determination …show more content…

The silver colloidal particles obtain a negative charge because of to the adsorbed citrate ions; The absorption peak was observed at about 420nm which is the typical characteristic absorption peak for Ag nanoparticles. UV absorption peak of chitosan-Ag nanoparticles was observed by other researchers in the range 410–450 …show more content…

The 1 µm size of chitosan conjugated silver nanoparticles confirmed their nano size structure.The profile of FTIR spectrum the main absorption of ch-agnps as 3345, 2123.71, 1639.43, 1045.09cm-1. The FTIR spectrum of the ch-agnps show OH stretch at 3345.78 and N-H bending at 1639 cm-1. In the FTIR spectrum of ch-agnps, the shift of chitosan peak is observed which shows amplification in the intensity of c-o stretchIn the present study all the clinical isolates were identified by observed their colony Morphology,s and biochemical characterization. as e.coli, p.aeruginosa, s.typhii and s. aureus. The results of this antibacterial activity of ch-ag np suggested that the presence of a small percentage of Ag nanoparticles in the composite was enough to enhance antibacterial activity significantly. Chitosan conjugated silver nanoparticles exhibit strong bacteriocidal activity at different concentration as compared to gentamycin. According to the result we can use chitosan conjugated silver nanoparticles to treat a various bacterial infection caused by pathogenic bacteria. Ch-AgNps can also help to reduce the problem of toxicity and to avoid the problem of multi drug resistance The broad spectrum of bioactivity of AgNPs makes them promising agents not only to fight. Both chitosan and silver nanoparticles are antibacterial agents

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