Kinetic Report

INTRODUCTION Gentamicin, which is a broad spectrum aminoglycoside antibiotic (Fig. 1), belongs to the class of medicinal compounds capable of inhibiting the growth of Gram-positive and Gram-negative bacteria [1]. Gentamicin is one of the most effective drugs used in the treatment of serious suppurative and septic processes, especially those that are caused by Gram-negative microorganisms. The advantages of gentamicin over other aminoglycosides (kanamycin, neomycin) are its activity towards Pseudomonas aeruginosa and microorganisms of the Serratia–Klebsiella–Enterobacter group, a faster bactericidal effect, and the rare development of gentamicin resistant strains. Several methods used for determination of GT including colorimetric [2,3], amperometric enzyme-immunosensors [4], flow injection chemiluminescence [5], spectrofluorometry [6,7], capillary electrophoresis with UV detection [8], liquid chromatography (LC) [9–14] and high performance liquid chromatography (HPLC) [15–23]. Kinetic methods have many advantages over other spectrophotometric methods due to their high selectivity and elimination of

2). All the reaction conditions including the concentration of the oxidant, amount of sodium hydroxide and temperature were studied. The reaction was utilized for developing two direct kinetic spectrophotometric determination of GT by monitoring the increase of the absorbance of manganate ion as a function of time at controlled temperature. These methods (A) and (B) are based on initial-slope (Method A) and fixed-time (Method B), and they were validated for linearity, sensitivity, accuracy and precision. The proposed methods were applied for the determination of GT in pharmaceutical preparations and the results were comparable with standard methods