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Iodine Value Lab Report

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INTRODUCTION Lipidsaareaa group of naturally occurringamolecules that includeafats, waxes,asterols, fat-soluble vitamins (such as vitamins A, D, E, and K) monoglycerides , diglycerides ,triglycerides, phospholipids, and others. The main biological functions of lipids include storing energy, signaling, and acting as structuralacomponents of cellamembranes. Lipids have applications in the cosmeticaand food industries as well as in nanotechnology. (Ackman, 1967) The iodine value (or "iodine adsorption value" or "iodine number" or "iodine index") in chemistry is the mass of iodine in grams that is consumed by 100 grams of a chemical substance. Iodine numbers are used for determining the amount of unsaturation in fatty …show more content…

Hanusaiodine solution, chloroform, aqueous KI solution, Na2S2O3 and starch solution is used. Iodine values are calculated from the difference between the blankaand the test sample. For peroxide value; solvent mixture (composed of glacial acetic acid and chloroform), saturated KI solution, starch solution and Na2S2O3 soluiton is used and peroxideavalues are calculated. A) Iodine Value: Hanus Method In this experiment, iodine value of sun floweraoil was determined with Hanusamethod. Blank solution and oil solution were prepared and stored in the dark. Then, they were titrated with Na2S2O3 and volume of titrant was determined for blank and oil solution. Finally, iodine value was determined by using volumeaof titrant. B) Determination of Peroxide Value In this experiment, peroxide value of sunflower oil was tried to determine. After preparing a solvent mixture, it was titrated with sodium thiosulphate but during titration time color change was not observed. The experiment was not completed successfully. METHODS AND MATERIALS A) Iodine Value: Hanus …show more content…

Then, putting of choloform in amount of 10 mL and solution of Hanus iodine as amount of 10 mL into conical flask is realized. Addition of these two substance into otheraflask also occurs for blank. Next, waiting for these two samples for exactly 30 minutes is realized. Afterthat, solution of potassium iodine in amount of 15 mL and 40 mL water being distilled are added. Titration of last mixture is performed in company with 0.1 M Na2S2O3 until the obtaining of color in yellow. Next, addition of starch indicator occurs and then titration of mixture in company with Na2S2O3 is realized until observing of color as blue is obtained. All of these procedure is repeated for solution of blank without adding oil. Consequently, quantity of iodine being used for absorption by oil is

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