INTRODUCTION:
Drotaverine is chemically1-[3,4 –diethoxyphenly)methylidene]-6,7-diethoxy-3,4-dihydro-2H-isoquinoline:hydrochloride[1] and empirical formula is [C24H31NO4.HCL][4] has no anticholinergic effects and is used as antispasmodic drug.specific used for smooth muscle spasm and pain. Chemically Ethamsylate is N-ethylamine2,5dihydroxybenzenesulphonate [2]. belongs to class of haemostatic compound that blocks prostacycline synthetase, an enzyme that converts arachidonic acid to Prostacycline and therefore increases platelet aggregation and platelet adhesiveness. It is used for the prevention and treatment of capillary haemorrhage, and postpartum haemorrhage. Ethamsylate is official present in British pharmacopoeia. [3]
Tranexamic acid
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1(A) Drotaverine hydrochloride 1(B) …show more content…
The analysis was carried on C18 shim- pack GIST (150mmx 4.6mm 5µ) column used as stationary phase. A freshly prepared mobile phase consisting of methanol: potassium dihydrogen phosphate buffer in ratio of (30:70 v/v), PH-3 adjusted using ortho phosphoric acid (OPA) these were filtered by 0.45µM Whatmann filter paper and sonicated before use. The flow rate of mobile phase was 1ml/min. The detection was carried out at 220 nm and run time was around 10 minutes.
Selection of wavelength
A UV spectrum of drotaverine hydrochloride, ethamsylate, tranexamic acid in water was noted by scanning the solution in the range of 200-400nm. drotaverine hydrochloride, ethamsylate, tranexamic acid was showing significant absorption at 220nm. thus that was selected as wavelength for analysis.
Preparation of standard stock solution
The standard stock solutions 100mg/ml each of drotaverine hydrochloride, ethamsylate, tranexamic acid was prepared than solution having concentration in range of 80-250µg/ml were prepared by diluting stock solution with mobile phase.
Preparation of sample