4.2 Antifreeze Fluids with IBPs As studies show in Dow Chemical Company (DCC), the glycerol-water solution becomes slushy when it freezes (107) indicated with our studies shown in Figure 41(a). Specifically, the experiment shows the interaction between 20% glycerol and IBPs. For example, at 274.2 K the right-side peak becomes shorter because it reaches its freezing point of the 20% glycerol at 268.5K (83). Then, when the temperature is lowered to 241.1 K, the inhomogeneity of the system due to the glycerol is observed. Studies from DCC has shown that when lowering the temperature, the slush becomes increasingly viscous (107). As shown in Figure 41(a-b), the interaction between glycerol and the protein is detected by the different line-shape