Prelab week 1 Calculations Preparation of 1.5μmol/L mixed low-level standard dilution 150μmol/L × V1=1.5μmol/L × 10ml V1=(1.5μmol/L×10ml)/(150μmol/L)=0.1ml Conversion of milliliters to microliters (0.1ml×1000)μL= 100μL Preparation of 3μmol/L mixed low-level standard dilution 150μmol/L × V1=3μmol/L × 10ml V1=(3μmol/L×10ml)/(150μmol/L)=0.2ml Conversion of milliliters to microliters (0.2ml×1000)μL= 200μL Preparation of 3μmol/L mixed low-level standard dilution 150μmol/L × V1=7.5μmol/L × 10ml V1=(7.5μmol/L×10ml)/(150μmol/L)=0.5ml Conversion of milliliters to microliters (0.5ml×1000)μL= 500μL Preparation of the blank samples The volumetric flask will be filled to the mark with 150μmole/L of stock solution to act as blank (reference). Additional two blanks will
= 10^-3 M = 1,000 mL Here C1,C2; are the first and second concentrations of solution V1 and V2 ; are the required and current volumes. The impeller turned on and DDA, and tap water left to be mixed properly with water for 2 minutes. Approximately 150 grams of quartz added into the solution.
The separated mixture was then filtered using vacuum filtration again. Once the filtrate was all pulled through the filter the remaining sand was washed twice with five mL of distilled water. Air was continuously pulled through until the sand was dry. The filtrate was transferred into another 150-mL beaker. The vacuum filtration was cleaned and set up again for the third filtration.
3- Next, add 3 mL of Ferric Chloride coagulant using cylinder tubes and 3 mL of Aluminium Sulphate to the beakers. Place the six beakers on the Flocculation apparatus and mix rapidly for one minute and slowly for fifteen minutes. Measure the time using a stopwatch and allow for another fifteen minutes to settle. 4-
Start the timer for 1 minute, and using a measuring cylinder, collect an outflow of 17-23 ml min-1. Record this initial flow rate. With this flow rate, make sure the large beaker is filled to the top with water and place the magnetic stirrer inside. Using a piece of rolled tissue, insert it into the sprout positioned above the smaller beaker. Turn on the magnetic turner and remove 2 milliliters of water and place it in a cuvette using a 5-milliliter autopipette.
Laboratory balance Glucose solution Nacl solution Congo red dye in 40%sucrose solution Paper towel Procedure 1. Number the beakers 1 to 4 with the wax marking pencil, and half fill all of them with the distilled water except beaker 2, to which you should add 125ml of 40%glucose solution. 2. Prepare the dialysis sacs one at a time by using the funnel, half fill each with 20ml of the specified liquid. 3.
In Section A, the average mass of the 50 ml beaker was weighed 3 times using 2 different electronic balances. The final average mass recorded was 33.73g. The volume of distilled water needed was calculated from the mass of water using the density= 0.9971g/mL in Section B. The volume of water needed was transferred using pipette, graduated cylinder and Erlenmeyer flask respectively. In Section C, a pipette was calibrated by measuring the water temperature and the density was determined.
Abstract This laboratory experiment investigated how different types of media plates affect the growth of skin microflora mainly microflora in the nostril It was hypothesized that the nostril microflora were gram-positive bacteria that belong to the genera Streptococcus, Staphylococcus and Propionibacteria. Results showed that the nostril microfloras were gram-positive (stained purple). However, gram staining was not enough to prove that the bacterium obtained from the nostril are Streptococcus, Staphylococcus, and Propionibacteria spp. The results do not fully support the hypothesis.
Next, we measured out 40mL of pond water 12 times using a plastic beaker, making sure our hands didn’t contaminate the sample, and then we poured the sample into a centrifuge tube.
The final volume was adjusted with the same solvent to get concentration of 100 µg/ml. the solution was further diluted to get the concentration of 10 µg/ml, filtered through 0.45 µm filter tips , and aliquots of 20 µl from this solution was injected into HPLC by using an
Volume of ethanol (10ml) 10 ml of ethanol solution was used for each experiment (except tube 1 for 100% ethanol concentration 20ml was used) Pipette is used for accurate
Weight a clean, dry, porcelain evaporating dish on the electric balance and record this mass on an appropriate data table. If the crucible needs to be washed before use, then heat the crucible in the Bunsen burner flame for a few minutes and remove any residual water. Then allow it to cool before continuing. Fill the crucible about 1 gram with the hydrated salt and reweight. Assemble the ring stand, ring, clay triangle, and Bunsen burner
Again select the flask and select Distillation Head from the drop down menu. ➢ For the third time select the flask and choose Condenser from Distillation from the menu and for last time select the flask Distillation Take-off from the dropdown option. ➢ Select the 100 mL Graduated Cylinder from the Equipment option and put it underneath of distillation take-off.
Introduction Chromatography is a laboratory technique for the separation of a mixture. The mixture is dissolved in a fluid called the mobile phase, which carries it through a structure holding another material called the stationary phase. The various constituents of the mixture travel at different speeds, causing them to be separated. In fact, the separation is based on differential partitioning between the mobile and stationary phases [1]. Chromatography may be preparative or analytical.
Introduction Buffer is a solution that resists a change in pH when bases or acid are added. Solutions that are acidic contain high concentrations of hydrogen ions (H+) and have pH values less than seven. Buffer usually consist of a weak acid, and its conjugate base or a weak base and its conjugate acid. The function of buffer is to resist the changes in hydrogen ion concentration as a result of internal and environmental factor. This buffer experiment is important so that we relies the important of buffer in our life.
