Omeprazole Research Paper

Omeprazole (RS)-6-methoxy-2-((4-methoxy-3, 5-dimethylpyridin-2-yl) methyl sulfinyl)-1H-benzoin (d) imidazole is a proton pump inhibitor (PPI) and an anti-secretory compound. It suppresses gastric acid secretion by inhibiting the gastric H+/K+atpase (hydrogen-potassium adenosine triphosphatase) at the secretory surface of the gastric parietal cell[1]. Omeprazole has been characterized as a gastric acid-pump inhibitor, in that it blocks the final step of acid production. After rapid disappearance from plasma, Omeprazole can be found within the gastric mucosa for a day or more. It is freely soluble in ethanol and methanol, slightly soluble in acetone and isopropanol and very slightly soluble in water. Omeprazole is useful in combination with the

The final volume was adjusted with the same solvent to get concentration of 100 µg/ml. the solution was further diluted to get the concentration of 10 µg/ml, filtered through 0.45 µm filter tips , and aliquots of 20 µl from this solution was injected into HPLC by using an

The method was developed on a Novapak C18 (250×4.6mm, 5µ particle size) column using phosphate buffer (pH7.4) and acetonitrile in the ratio of 60:40, v/v as a mobile phase which was pumped at flow rate of 1.0m/min and detection was done at 302nm.the retention time of drug was found to be 7.71min.A prominence isocratic HPLC system (HPLC YL9000 series) with autochro 3000 software an uv detector. A 20 µl hamitton injection syringe was used for sample injection.
Preparation of standard and stock solutions 40 mg of Omeprazole was weighed and transferred to 100ml volumetric flask containing 30 ml of 0.1NNaoH. The drug was allowed to dissolved and volume was made up to 100ml with 0.1 Noah. The above solution was diluted with mobile pause to obtain a working standard solution of 40 µg/ml. The blank solution was prepared and injected. The prepared standard solution was injected and the chromatogram was recorded