Dependent Variable amount of product (glucose and fructose) produced 2. Independent Variable temperature 3. Controlled Variables pH, amount of substrate (sucrose) present, sucrase + sucrose incubation time Effect of Substrate Concentration on Enzyme Activity 1. Dependent Variable amount of product (glucose and fructose) produced 2.
The final mass could be far off due to the water and chunks of expanded gummy bears found in the beaker, leading to an inaccurate result. As well, for the sugar solution, the result could have been different if a more accurate measurement of the sugar needed was made. For the specific result, the hypothesis stated, the sugar solution needed to have an equal amount of sugar content to the gummy bear which did not occur. Ensuring that the beaker contained 10 grams of sugar was off, due to prerequisite calculations that lead to too much liquid in the beaker that needed to be removed. To be correct, the hypotheses that were wrong could
3. In this experiment, the percent yield was 90%. This number implies that there was little error in this experiment. However, this result could have been caused by certain external factors.
I predicted that the control would have a higher alcohol content than the experimental since beta and alpha amylase are working together. Since only Alpha-Amylase worked in the experimental, there was probably bigger carbohydrates present in the flask, therefore, there was a lower alcohol percentage since yeast can’t digest bigger sugars. b. My results also matched my prediction regarding mean reducing carbohydrate levels during the mashing process between the control and the experimental. My prediction stated that there would be less reducing carbohydrate ends in the experimental, which was proven in the data table.
In the lab “All That Glitters” the objective that was focused on during the lab was calculating the density, volume and mass of various substances. The method that was used in finding the volume of the samples is called the displacement method. This is a process where the volume of the water in the graduated cylinder is calculated before and after the sample is placed. In this lab, the goal of the experiment was to identify and come to consensus about what the unknown substance might be. For this experiment, the required materials were ten pre and post pennies, unknown sample, graduated cylinder, weigh boat, water, paper towels and a weighing scale.
The dough troughs are large, stainless steel oblong containers with rounded bottoms and are mounted on wheels, to facilitate their being rolled into the fermentation room. Here, where the temperature is held at 80 degrees F., the sponge ferments for about five hours. During this time, there is a chemical interaction of carbonates and acids, causing the sponge to rise. At the completion of the fermentation period, the sponge is ready for the next step in the method, mixing the dough. The sponge is returned to the mixer together with three hundred pounds of flour, 240 pounds of water, nonfat milk solids, and sugar.
Next, weigh out 5 grams of sugar and 1 gram of yeast. Once in proportions, add it to test tube A. Thoroughly mix around the sugar, yeast, and tap water. You can do this by shaking the test tube gently. Make sure you do not turn it upside down. Then, cover the top of the test tube with a balloon and gently place it into a 400mL beaker filled up about half way (about 250mL) with water.
o For all three trails the H202 solution in water increase by 10˚C in terms of before and after yeast is added. o The third trial has the same trend of increase as the first two but begins and ends with a 1˚C higher than the previous trials. Data processing: Number of moles for the hydrogen peroxide (H202) 34.02 =
Only the heated solution caused the balloon to expand, suggesting that the increase in temperature is linked to the balloon’s expansion. Furthermore, as the solution was only heated to 60°C, no water vapor was produced to fill the balloon, suggesting the gas was produced solely by the yeast. Thus, the yeast reacted to the heat, supporting the claim that yeast can respond and is alive. Sources of error in this experiment could have included incorrect preparation of solutions. The solutions of yeast, water, and sugar, could have been measured incorrectly causing the control and experimental solutions to be different.
Hypothesis I predict that the more baking powder is added to the vinegar, the
Joshua Miller 12/18/17 Fermentation Lab report Introduction The term fermentation refers to the chemical breakdown of a substance by bacteria, yeasts, or other microorganisms, typically involving effervescence and the giving off of heat (wikipedia). Sugars are converted to ethyl alcohol when fermentation happens. In this experiment we determined if yeast cells undergo fermentation when placed in a closed flask with no oxygen. Glucose and yeast are mixed together in a closed flask and allowed to incubate for about one hour.
5 water bath were set up each to10 °C. (5 were used do the experiment faster) 5 cm3 of starch solution were added into the 5 test tubes that were labeled test tubes. Then 5 cm3 of amylase enzyme was added into the other 5 test tubes that were labeled. Put one of the starch solution test tube (preferably the one labeled 1) and one of the test tube containing amylase into the water bath (10 °C).
1% glucose, 1% maltose and 1% lactose all progressively get positive results by changing colours to reddish brown at the end of this experiment. In this case the aldehyde functional group that is present in the products (monosaccharides and some disaccharides) in this reaction is able to reduce copper in the presence of alkali and this produces colour changes while converting to an aldose sugar. Honey is made of fructose and glucose which instantly turned brown after the test-tube was placed in the boiling water because of its active aldehyde and carbonyl group. The copper (II) sulphate present in the Benedict’s solution reacts with electrons from the aldehyde group which results in a redox reaction to from cuprous oxide, a red brown precipitate that seen in all of the above mentioned solutions (Hill, 1982). Beer also gave positive results because it contains aldehydes and ketones (i.e. acetone, trans-2-butenal, furfual) during its beer production process where the sugars are converted through fermentation (Hill, 1982).
Abstract The distinction between baking soda and baking powder is acid. At any rate it can have a major effect for different products, so we should clarify. Both baking soda and baking powder are leavening agents, which imply that they are added to heated products before cooking to create carbon dioxide and the reason for them to increase. The two substances are utilized under diverse conditions.
What is the effect of temperatures 10°C , 20°C, 40°C, 60°C and 70°C ± 1/°C on yeast fermentation when baking bread? ii. Aim: The focal aim of this experiment is to investigate the effect that temperature has on the growth and respiration of yeast (Saccharomyces cerevisiae) fermentation. iii.
