Birby Bauer Disk Diffusion Report
However, differences were not significant to each other. The increased of bacterial count may be attributed to the application of EM. EM contains different types of bacteria and these bacteria were added to the bacteria that were already in the water samples before the application of EM. Two weeks after EM application, treatment 2 becomes the highest in bacterial count followed by treatment 3 and lastly treatment 1. But after three weeks after EM application, treatment 1 contains the highest bacterial count followed by treatment 3 and treatment 2, respectively.
Diffusion and Osmosis Lab Report By: Jettica Williams BIOL 1107 Lab September 21, 2016 Prepared for Mrs. Fulford Lab Course Page Break The cell membrane act as a roadblock for cells. The cell membrane has a very hectic job. It restricts the access to what comes in and what goes out. The bond the membrane shares with others is the idea of accountability.
Osmosis is a process where the solvent molecules pass through a semipermeable membrane from an area of high concentration to an area of low concentration until it reaches equilibrium. These solvent molecules, typically water, move freely. Carbon dioxide is able to move across the cell membrane through a process called simple diffusion. This works in a similar manner, carbon dioxide is able to move freely across the cell membrane from low to high concentration. When oxygen is breathed in, the red blood cells in the lungs have a low concentration of oxygen and a high concentration of carbon dioxide.
The purpose of this experiment is to compare different types of water, including, well water, city water, bottled water, and water from a water fountain. I plan to take tap water from multiple faucets, with some using well water and others using city, use a brand of bottled water, and samples from a water fountain to grow bacteria. “Water is an excellent solvent and can be sourced from almost anywhere on Earth. This property makes it prone to all kinds
However, when these bacteria are grouped together to have high cell density, the molecules they secrete amount to a certain number, and once that number is reached, the behavior of that bacteria is switched on and in this case, bioluminescence is created. Similarly, in my project, I am screening the anti bacterial activity using oils. Before I use the oil, I have to culture the bacteria overnight so that I could use them in the plates after 16-18 hours of incubation. Based on the talk, I believe that I have an idea on how bacteria grow. This Ted Talk has inspired me about science in numerous aspects.
What is Bulging Disc? A bulging disc is a typical spine injury that is sustained to intervertebral plate of your spine. It can happen in your lumbar spine, which is at the lower back, thoracic spine (upper and mid-back) or your cervical spine (neck). The bulging disc can normally be alluded to as a slipped disc.
The purpose of gram stain and culture is to guide antimicrobial therapy. The laboratory will typically test bacterial growth against commonly prescribed antibiotics and determine resistance patterns. Bacterial cultures generally take at least 2 days to
Anti-bacterial agents kill or inhibit bacterial growth. My conductive research consisted of making agar, growing bacteria that I received from the inside of a human’s mouth and using different toothpastes to see which brands work better. Introducing bacteria
Staphylococcus aureus Introduction/ Background information Staphylococcus aureus (S. aureus) is yellow-pigmented colonies and this is a reason for (aureus = golden) in its genus name. S. aureus is a spherical shape (Coccus), gram positive bacterium that usually present in pairs as seen in microscope.it has short chains, or bunched, grape-like clusters, non-motile, no spores and facultative anaerobic. Members of this species can survive in the aerobic or anaerobic conditions and they can adapt to any of situations. The best environment for them to grow is in dilute salt concentrations and low moisture, which partially introduces human's nasal secretions and skin's surface to be a suitable place for this microorganism to grow. This bacterial growth can lead to staphylococcal infections in skin, nose and throat of human
The Effect of the Molecular Weight of a Substance to its Rate of Diffusion Paulo E. Garcia Group 2 Sec U-7L October 16, 2014 ABSTRACT The rate of diffusion was tested by using a petri dish with agar-water gel and three drops of different solutions. These solutions were observed for thirty minutes and measured every three minutes. The different solutions used are Potassium permanganate with a molecular weight of 158 g/mole diffused the greatest distance among the three, Potassium dichromate with a molecular weight of 294 g/mole diffused the lesser than that of Potassium permanganate, and Methylene Blue with a molecular weight of 374 g/mole diffused the least distance.
The pictures that were incorporated in each section give a visualization of what each section is about, and what to do in each situation; even a child or illiterate person can have an idea what the brochure is all about (www.jointcommission.org). This topic interests me because hand hygiene and infection control as a whole, have great importance in my daily life. Since health care-associated infections (HAIs) result from the delivery of health services in a health care facilities, as a nursing student this information will help me to be more aware to always maintain infection control when providing care to my patients to prevent the spread of infections (Potter, 2013).
The testing for affectability of a life form to antimicrobial agent is normally done utilizing agar dissemination or disk diffusion test. The parameters of this test were indicated (or institutionalized) by the researchers W. M. M. Kirby and A. W. Bauer and is likewise alluded to as the Kirby-Bauer antibiotic testing. In this technique, anti-toxins or antibiotic are impregnated on a specific extraordinary kind of paper circles and are put on the surface of agar containing the bacterium and parasitic (fungi) of our interest. This outcomes in the dispersion of antimicrobial agent into the surrounding medium. The diameter of the zone of inhibition will decide the adequacy or sensitivity of the antibiotic; the bigger the diameter, the more
Gram-Positive bacteria that are inhibited with MacConkey agar due to crystal violet and bile salt presence . “3. What ingredient(s) makes MacConkey agar differential?” MacConkey agar are inhibited by a pH indicator (neutral red), and a disaccharide ( Lactose) “4.
ıt is easy to prepare. It provides the environmental and nutritional conditions similar to their natural habitat. It is useful when a specimen contains a mixed bacterial colonies to achieve distinct colony-forming units. It also has disadvantages like it does not support the simultaneous growth of aerobic and anaerobic bacteria. Consequently, duplicate plates must be inoculated, and incubated in both atmospheres.
aureus bacteria did not develop resistance to lugdunin, even though with the exposure in people and in repeated exposure in the laboratory. In mice, S. aureus causes lugdunin applied topically was able to either partially or totally eradicate skin infections. This antibiotic cannot be used to clear S. aureus colonies in people recently. The researchers trust that the drug might be able to be taken systemically as it did not exhibit any signs of toxicity on a sample of human serum. Furthermore, lugdunin is not very soluble in water because it might be difficult for body to absorb it.
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