Disk Diffusion Test Lab Report

The testing for affectability of a life form to antimicrobial agent is normally done utilizing agar dissemination or disk diffusion test. The parameters of this test were indicated (or institutionalized) by the researchers W. M. M. Kirby and A. W. Bauer and is likewise alluded to as the Kirby-Bauer antibiotic testing. In this technique, anti-toxins or antibiotic are impregnated on a specific extraordinary kind of paper circles and are put on the surface of agar containing the bacterium and parasitic (fungi) of our interest. This outcomes in the dispersion of antimicrobial agent into the surrounding medium. The diameter of the zone of inhibition will decide the adequacy or sensitivity of the antibiotic; the bigger the diameter, the more

Cycloheximide applies its impact by interfering with the translocation steps in protein synthesize (development of two tRNA atoms and mRNA in connection to the ribosome), hence blocking translational prolongation. Cycloheximide is generally utilized as a part of biomedical research to repress protein synthesize in eukaryotic cells except for S.aureus and E.coli contemplated in vitro (i.e. outside of microorganism). It is cheap and works quickly. Actually after the interaction of 72 hours, both growth of E.coli and S.aureus will be inhibited by Cycloheximide antibiotic. But for our experiment, E.coli shows negative result which means it is resistant toward Cycloheximide and do not producing a clear zone. Maybe the concentration of Cycloheximide is too low when it was plated on the E.coli, thus it cannot be inhibited by the antibiotic. The inbition could not be seen in both fungal species 2 and 3 also and this might be because of there was no fungal colony on the cut agar due to the poor lawn on the agar

Anti-infection agents are utilized to treat diseases caused by bacteria mostly Gram-negative bacteria. Nalidixic acid works by killing the microbes that are causing a contamination or infection. It does this by entering the bacterial cells and restraining a bacterial protein called DNA-gyrase. This catalyst is associated with duplicating and repairing the genetic material (DNA) of the microbes. On the off chance that this enzyme doesn't work, the microbes can't replicate or repair themselves and this executes them.Nalidixic acid is filtered out of the blood and pass into the urine by the kidneys. Large amounts of the prescription go into the urine, which implies nalidixic acid can be utilized to treat bacterial contaminations in the urinary tract. But as for my practical, we got clearing zone of S.aureus instead of E.coli. Since the inhibition supposed to go to E.coli but maybe due to some technical error like too high concentration of Nalidixic acid when plated on S.aureus compared to E.coli, that is why we got false positive