Escherichia coli Essays

However, certain strains of E. coli are of serious concern to the medical community due to its capability for opportunistic infection leading to a wide variety of severe gastrointestinal diseases [CITATION 2]. The bacterial unknown in this experiment was identified as Staphylococcus

normally used cut foreign DNA and placed it into the plasmid vectors. This lab used Escherichia coli (E. coli) bacteria (Kok , 19840). This is because Escherichia coli can be simply grown in Luria broth or on agar, and also has a comparatively small genome of five million base pairs.

Enterococcus durans, and Escherichia coli. Citrobacter freundii is a Gram-negative rod shape bacteria. The MSA plate will grow Citrobacter freundii, Bacillus subtillis, Enterobacter aerogenes and will have a yellow color change while Staphylococcus epidermis will not grow nor have a color change to yellow. The MacConkey agar will have growth with Escherichia coli, Enterococcus durans, but not Staphylococcus epidermis and Bacillus subtilis since it is a Gram-positive. Only Escherichia coli and Enterococcus

incubation overnight. [1] Colistin is an antibiotic belonging to the polymyxin group of antibiotics, and is used to treat gram negative bacterial infections. [2] In this experiment we tested the antibiotic Colistin at several concentrations against Escherichia coli. This was done to find out the minimum inhibitory concentration (MIC) needed for Colistin to work effectively as a bactericidal. The postulated MIC for Colistin was 2.0 micrograms per milliliter. To test this postulated MIC, several serial dilutions

Serratia: Serratia’s a group of gram (-negative), facultative anaerobic, rod-shaped bacteria of the Enterobacteriaceae family. Serratia are most common and pathogenic of the species within the genus, S. marcescens, is often the sole infective agent and frequently causes health facility infections. S. marcescens is often found in showers, bathroom bowls, and around wetted tiles. The bacterium is associate timeserving, human infective agent, capitalizing on its ability to make integrated surface communities

“STUDY OF VIRULENCE FACTORS AND ANTIBIOTIC SENSITIVITY PATTERN IN ESCHERICHIA COLI STRAINS ISOLATED FROM EXTRAINTESTINAL INFECTIONS” INTRODUCTION Escherichia coli (E. coli) is one of the most important members of the family Enterobacteriaceae and a commensal in the human intestinal tract. (1) As a commensal it contributes to the maintenance of health of a person. However, E.coli when enters into unnatural sites, can cause variety of conditions such as urinary tract infections, wound infections,

Escherichia Coli 0157:H7 This paper will specialize on a specific type of bacterial foodborne illness caused by the bacteria Escherichia Coli. E. coli was discovered by Theodore von Escherich in 1885. E.coli is a natural found bacteria that lies throughout the intestinal tract of warm blooded animals and comes in many forms only one of which is deadly. This form is E. coli 0157:H7 which can be caused by direct exposure to fecal matter to kill this rouge E.coli the contaminated material must be cooked

Worldwide the number of beef cattle being slaughtered who were contaminated with strains of E. Coli ranged from 2.1 to 70.1% (Clark). Diseases that can infect humans have been manifesting within the beef cattle industry for decade resulting in thousands of deaths for humans. Two of more common infections include E. Coli and Mad Cow Disease both of which are consequences of how cattle are raised and treatment in factory farms. Because of the lack of procedures being followed and standards being upheld

palm (Phoenix dactylifera) plants were purchased from local market in Duba province, west Saudi Arabia were assayed for the in vitro antibacterial activity against three human pathogenic bacteria viz Klebsiella pneumoniae, Psuedomonas sp, and Escherichia coli using hole plate diffusion method. The result revealed that some ethanolic and hot water extracts had shown antibacterial activities against the three bacterial strains under investigation. Key words: plant extracts, coffee, black cumin, date

to agriculture. In this lab we will be inserting a gene into an Escherichia coli bacteria with the help of a plasmid. Escherichia coli bacteria also known as E. coli, is a bacterium that is rod shaped and contains flagella to help it move. The bacterial DNA is circular inside of an E. coli bacterium. E coli. is most known for being found in the intestine of humans and animals but it can also be found in other places such as food

Escherichia coli (E. coli) is normally found in living intestine of humans and animals. A bacterium that are harmless and is important to the health of the human’s intestinal tract. The intestinal tract is part of the digestive system; having varies of sizes of intestine that transport the food in order to absorb the nutrients and expel the waste. However, some E. coli are pathogenic, causing illness to the host such as diarrhea or harm the outside of the intestinal tract. The host can be effected

dengue and tuberculosis which is 62.5 cases per 100,000 population in 2008. The example of foodborne disease is Botalism, Campylobacteriosis, E.coli, Hepatitis A., Norovirus Infection, Salmonellosis, Shigellosis and others. E. coli E. coli are known as Escherichia coli which is a bacteria are usually can be found as part of the normal gut flora in the large intestines of mammals such as cows and sheep. There are certainly some deadly strains in its ranks. But most E.coli are harmless. Billions

“Post-Lab Questions” What ingredient(s) makes MacConkey agar selective for Gram-negative bacteria? The ingredients that make MacConkey’s Agar selective is a pH indicator (neutral red), and a disaccharide ( Lactose) “2. What types of bacteria are inhibited on MacConkey agar?” Gram-Positive bacteria that are inhibited with MacConkey agar due to crystal violet and bile salt presence . “3. What ingredient(s) makes MacConkey agar differential?” MacConkey agar are inhibited by a pH indicator (neutral

is typically caused by Escherichia coli (E. coli) or Staphylococcus

trp and ara operon trp operon: The trp operon is a group of genes that are used, or transcribed, together that codes for the components for production of tryptophan. The trp operon is present in many bacteria, but was first characterized in Escherichia coli. The operon is regulated so that when tryptophan synthesis are not expressed. It was an important experimental system for learning about gene regulation, and is commonly used to teach gene regulation. Discovered in 1953 by Jacques Monod and

coli was used in this experiment due to its versatility and usefulness in biological research. E. coli have been used for a long period of time as the subject organism in many biology experiments, making it one the most understand organism. Additionally, E. coli is relatively harmless to the researchers because it is naturally found in the intestines of humans. Furthermore, it has a very fast growth rate, capable of doubling its population every 20 minutes (Lee, 1996). E. coli also contains

removed the person must get a prescription from a doctor for an antibiotic. Deficiencies and Causes An infection usually occurs when the bacteria, Escherichia coli (E. coli), gets into the urine and begins to grow. The infection starts at the opening of the urethra where the urine leaves and moves upward into the urinary tract where it begins. E. coli lives in the colon and around the anus where it is then moved to the urethra. The bacteria travel up the urethra into the bladder, where the infection

Contamination with pathogenic bacteria from the human/animal reservoir can occur when the landing place is unhygienic or when the fish are washed with contaminated water. Upon the processing of fish, its exposure to water is a critical part as pathogens may be present in the water used which can cause contamination and spoilage (Shoolongela & Schneider, 2012). Contamination of food can occur because of problems in food production, preparation, storage or cooking. The aquatic environment and soft

Exponential Growth of E. coli Escherichia coli, also more commonly known by the name E.coli, is a commonly found in the large intestines of warm-blooded organisms. The strains of bacteria that are found in the body are not harmful and can actually aid the body in things like digestion. E.coli, like a lot of bacteria, have a growth stage that can be expressed exponentially in both an equation and on a graph. In order to calculate and predict the number of E. coli bacteria cells growing on a plate

The results for my organism Escherichia coli consist of performing several tests to determine the organism’s characteristics. One of the tests I chose to perform is a starch hydrolysis test this is a differential test to help determine if the organism can produce enzymes amylase used absorb starch. This test helps to determine if E. coli can break down starch, and if it can we know that it has the ability of producing coenzymes called Amylase use in breaking down glucose bonds. If the organism can