ipl-logo

Antir Spectroscopy Lab Report

1115 Words5 Pages

FTIR Spectroscopic Study on Quantitation of Urea in Human BloodSerum
Abstract: The quantitation of urea has been achieved using FTIR spectroscopy. The FTIR spectra of human blood serum samples are recorded in Mid IR region 4000-400 cm-1.The normal blood serum is treated with urea at different concentrations and FTIR spectra are recorded, which confirm the specific peaks related to urea. A plot between concentration of urea and percentage of absorbancehas shown linear relationship. The study being complementary to chemical analysis is very much useful for the estimation of urea in the blood serum of patients suffering from diabetes and renal diseases.

Key Words: FTIR Spectroscopy, Quantitation, Human blood, Serum, Urea.

INTRODUCTION: IR spectroscopy has been used by Biophysicist and Chemist as a powerful tool to characterize …show more content…

2. MATERIALS AND METHODS:
The blood samples were collected from healthy donors without adding anticoagulant and serum was separated by removing blood clot. The serum was treated with research grade (SD Fine Chem) glucose at the concentrations of 100,200,300,400 and 500 gm/dL. Infrared spectrum was recorded in FTIR spectrophotometer (ShimadzuFTIR - 8400S) in the range of 4000 cm-1 to 400 cm-1. The resolution was kept at 4 cm-1 and scanning time was fixed at38 Sec. A total number of 10 scans were carried out on each sample.

3. RESULTS ANDDISCUSSION:
Fig.1. shows FTIR spectra of human blood serum treated with Urea of concentrations 100, 200, 300, 400, 500 mg/dl. Spectra show a series of bands, in the range of 4000 cm-1 and 400 cm-1, related to functional groups of proteins, lipids and carbohydrates and other inorganic materials present in the blood serum. Characteristic band for Urea can be seen approximately at 1085 cm-1.

Wave Number (cm-1)
Fig.1.(a). FTIR spectrum of human blood serum treated with Urea of concentration
100

Show More
Related
402 Words | 2 Pages

However this test has a low sensitivity where some individuals with low result would be considered to be deficient but show no clinical evidence of deficiency and conversely symptoms of deficiency can be seem when the result does not fall into the low range. There is a large ‘grey zone’ or ‘indeterminate range’ between normal and abnormal levels. In order to detect vitamin B12 deficiency, a more sensitive and specific screening test is required. Haptocorrin (HC) and transcobalamin (TC) are transport proteins for vitamin B12 . Transport of vitamin B12 to the tissues is brought about by TC.

Read More
1366 Words | 6 Pages

Cadet Eric Wiggins Date: 18 September 2014 Course Name: Chem 100 Instructor: Captain Zuniga Section: M3A Identification of a Copper Mineral Intro Minerals are elements or compounds that are created in the Earth by geological processes. The method of isolating metals in a compound mineral is normally conducted through two processes.

Read More
704 Words | 3 Pages

Pure ASA crystals are isolated from the solution with a Hirsch Funnel that was used with a filter. The melting point of the pure ASA crystals were calculated in order to calculate of absorbance. Iron (III) salicylate dianion must contain the acidified solution Fe3+ in order to measure the absorbance values. The level of the impurity can

Read More
1545 Words | 7 Pages

Introduction The purpose of this lab was to use chemical and physical tests to identify indicators of disease in synthetic urine samples. This lab tested samples for protein levels, glucose levels, and pH levels. In a normally functioning individual, proteins cannot pass through the glomerulus; therefore proteins should not be found in urine. However, in the nephrons of individuals with Bright’s Disease, the glomerulus no longer stops all proteins from entering the urine (Giuseppe et al., 2002, pp.

Read More
335 Words | 2 Pages

K.D.A. Saboia et al. , (2007) have been prepared the Bi4Ti3O12–CaCu3Ti4O12 {[BIT(X)–CCTO(100-X)]} composite powders through solid state reaction method and calcined in the range of 900 to 1020 ºC for 12 h. The as-prepared powders have modified in the form of thick film onto alumina ceramic substrate by utilizing screen printing. At 100 Hz, the value of dielectric constant (κ) of CCTO100 and BIT100 is 316.61 and 53.64 respectively. Conversely, the composite with X=20 % shows an unexpected dielectric constant of 409.71, which is around 20% higher in comparison with the CCTO.

Read More
1090 Words | 5 Pages

ABSTRACT To catalyze a reaction, an enzyme will grab on (bind) to one or more reactant molecules. In this experiment we examined how increasing the volume of the extract added to the reaction would affect the rate of the reaction. The enzyme used was horseradish peroxidase which helps catalyze hydrogen peroxide. Using different pH levels, the absorbance rate of the reaction was measured to see at which condition the enzyme worked best. The rates of absorption were calculated using a spectrophotometer in 20 second intervals up to 120 seconds.

Read More
1764 Words | 8 Pages

1.1 Abstract The purpose of quantitative analysis of protein using a spectrophotometer is to measure the concentration of proteins in a given sample. The experiment is conducted by laboratory method (Biuret Test) and using spectrophotometer to analyze the absorbance of reactants at 540 nm, hence determining the concentration of the proteins in a given sample. The purpose of stopped enzyme assay to study B-galactosidase is to determine the effect of temperature and concentrations of substrate on enzyme activity.

Read More
1567 Words | 7 Pages

When this happens the urine will turn a dark blue-black color when it is exposed to air. Therefore it may not look abnormal as soon as it exits the body but actually turns colors if it sets for a period of time so that it is exposed to air to express the blue black color. Researchers define a high urinary level of homogentisic acid as being greater than four to eight grams in a twenty four hour period. With children dark staining in the diapers is a sign that homogentisic acid is present and being excreted and this can indicate that the child may have the AKU disorder. It is important to note that many patients do not show many signs and do not become aware of their disorder until they are in their third or fourth decade of life.

Read More
3223 Words | 13 Pages

The mobile phase used was a mixture of ammonium acetate buffer and acetonitrile at a ratio of 400:600. A flow rate of 1 mL/min was maintained, and the detection wavelength was 292 nm (22). The required studies were carried out to estimate the precision and accuracy of the HPLC method and were found to be within limits [percent coefficient of variation was less than 15%]. Sample preparation briefly involved 0.4 μ membrane filter through which the sample was filtered, diluted with mobile phase, and 10 μL was spiked into

Read More
1232 Words | 5 Pages

1. Hct – Hematocrit, also called packed-cell volume (PCV), is the proportion of the total blood volume that is composed of red blood cells, or erythrocytes, that transport oxygen throughout the body Normal : 38.8 to 50% (men), 34.9 to 44.5% (women) Low = A lower than normal hematocrit may indicate: • An insufficient supply of healthy red blood cells (anemia) • Vitamin or mineral deficiencies • Recent or long-term blood loss High

Read More
681 Words | 3 Pages

As regards the first aspect, it happens that in hemolytic uremic syndrome the blood within the capillaries, the smallest blood vessels in the body, begins to coagulate in an abnormal way. When red blood cells pass through the capillaries clogged, they are punched from one side and broken. (Hemo-blood + lysis-destruction). The second

Read More
398 Words | 2 Pages

The first stage is asymptomatic hyperuricaemia where levels of uric acid

Read More
830 Words | 4 Pages

After treatment with L-thyroxine, the pressure marker is reduced to a considerable degree. MDA can also be used as a useful biomarker to measure and monitor oxidative stress. The role of antichrist in the form of selenium is incomplete (5). In our research we find antioxidant status in hypothyroidism Material and

Read More
1437 Words | 6 Pages

The reaction that occurs can be investigated via the adding of the liver extract which contains the arginase to urea concentrations and distilled water. The amount of urea formed is determined via spectrophotometric analysis α-INPP. The urea produced was known via the color reaction with the α-INPP, it is the reagent used for the colorimetric determination of urea. (Barry J, et al. 1984). The red color formed when the α-INPP is reacted with the urea, is sensitive to light thus it is photo labile.

Read More
3218 Words | 13 Pages

Based on this fact, the primary screening method for all forms of thalassemia relies on red blood cell parameters index cut-offs, which involves an accurate blood count using an electronic blood cell counter. A commonly used approach consisted of a complete blood count to assess the MCV and the MCH (Metcalfe, 2007). The finding of a normal MCV (80fL) in combination with a normal MCH (27pg) would rule out most cases of thalassemia and would require no additional thalassemia testing. Individuals with MCV of less than 80fl and MCH of less than 27pg should be examined further to confirm or exclude the diagnosis of both alpha and beta-thalassemia (The Thalassemia Working Party of the BCSH General Haematology Task Force, 1994; A Working Party of the General Haematology Task Force of the British Committee for Standards in Haematology, 1998). Some screening programs rely on the identification of low MCV alone in the absence of iron deficiency (Ronald, 2006; Ashraf, 2004).

Read More

More about Antir Spectroscopy Lab Report

Related Topics

Open Document