Cobalt Synthesis Lab Report
Introduction: In the world, there are a lot of important materials that we use in our everyday lives. Aluminum is a major metal that has many uses and is very helpful in achieving things through the use of recycling. The purpose of this experiment was to show how aluminum undergoes many chemical reactions to produce Alum. Materials and Methods: When this recycling experiment was conducted, we first had to gather the essential material needed.
In order to properly appreciate the importance of aluminum recycling in our world, an aluminum recycling experiment was done through alum synthesis. The process by which this was done recycled solid aluminum can pieces (Al) into the form of solid raw alum crystals (KAl(SO4)2) through a series of reactions. Application of this experiment comes from analyzing the calculated percent yield of alum crystals, understanding where error is involved, and determining how a perfect yield could be obtained. First, 0.9-1.2 grams of aluminum pieces were weighed out and placed in a 250 mL beaker, in which 50 mL of 1.4 M KOH solution was added and a fume hood was placed. The mixture was placed on a hot plate and stirred to speed up the reaction.
After the spectrometer was calibrated, 5 mL of yellow dye was placed in another cuvette from the erlenmeyer flask using a 5 mL transfer pipette. The absorption level of each wavelength of the yellow dye was then recorded using Logger Pro, along with the peak absorption and its corresponding wavelength. Afterwards, the dye was discarded and the cuvette
The purpose of this experiment is to learn about the principles of protein assays as well as to learn how to utilize the Beer-Lambert Law by doing various calculations such as how to calculate absorbencies, concentrations, and extinction coefficients. According to the Beer Lambert Law, absorbance is proportional to path length and concentration. For this experiment we will be learning how to use a spectrophotometer which measures transmitted light intensity. Spectrophotometers measure wavelength based on the color produced. In addition, we will be using standard curves to calculate protein concentrations.
The experiment required us to test first the relationship between wavelength of light and absorbance. The chloroplast pigments from the tissue were used to see which wavelength of light absorbed the most light. The result of this part of the experiment showed that the wavelength 432nm had the highest absorbance of 0.286. Next, we tested the absorbance of different colored lights. This part of the experiment tested the results of absorbance of red, green, white, purple, blue and no light using DCIP.
(Larsen, n.d.). A protein can be quantitatively assayed by many spectrophotometric methods. Generally, most methods used are by a chromophore. A chromophore is the color that appears when a molecule absorbs a specific wavelength of visible light and transmits or reflects others. (Encyclopædia Britannica,
Introduction: Iron is a grey, reflective metal element which is commonly used in infrastructure. Many buildings and everyday objects are made of iron as it is strong, common, has a high melting point and holds its shape. It is a favourite metal used in knives and axes because of how it holds its shape in a point for a long time and is cheap compared to metals such as titanium. The framework of buildings and machinery are just a few examples of how iron is used in a thick and sturdy form. For the purpose of the experiment iron wool was used as its thin strings allow water and oxygen to have better access to the metal therefore accelerating the oxidation process.
Multiple experiments were conducted to test the rate at which O2 production was influenced by the availability of inorganic carbon. In all experiments, a varying concentration of (either 0.1%, 0.4%, 0.6%, 0.8%, or 1.0%) sodium bicarbonate was assigned to each lab bench, to use as the solution in which the aquatic plants were submerged. In the experiment my partner and I conducted, ~225 mL of distilled water was poured into two 250 mL Erlenmeyer flasks. These would later be used as photosynthetic systems— an experimental control to regulate temperature changes.
As a young man, Ira Remsen was intrigued by science, leading him to perform an experiment that he saw in a textbook. This experiment involves a series of chemistry topics, triggered by the reaction that occurs between nitric acid and copper. The experiment involves an elevated flask, plug, a hose, and a container of water the set up is diagrammed below: Initially, place a copper penny in the flask then, standing by with the hose and plug, pour nitric acid into the flask and immediately seal. A green liquid and red gas will form inside the flask, until the pressure decreases, the water is forced up into the flask from the container below. When the water enters the flask, it neutralizes the solution in the bottom and dissolves the noxious gas.
Introduction Aluminum is a significant good in modern society due to its many uses in commercial goods. Because aluminum is consumed so rapidly, it becomes somewhat expensive to extract it from its original source. In recent years, there has been a push toward the recycling of aluminum through electrolytic reactions resulting in significant decreases in cost and aluminum production. Materials and Methods Using a scoopula 1.2141 grams of aluminum can pieces were weighted on an analytical balance and placed inside a 250mL beaker. On end of a rubber cord was then connected to the aspirator (located on the lab table) while the other end was connected to a funnel, functioning as a fume hood.
Chromatography Lab Riley Borklund Table 5, Seat 2A Lab Partners: Martin, Katherine, and Dakari Honors Biology, Mrs. Semaan January 5, 2016 Abstract: The purpose of this lab is to find what pigments are in a spinach leaf. The only pigments visible to the eye are chlorophyll a and chlorophyll b. We know this because chlorophyll reflects the green wavelength of light and shows us that it is present. We also, however, wanted to know what else is present in the spinach leaf.
In this lab we demonstrated a series of sequential steps of chemical reactions in an enclosed system, to detect whether the Law of Conservation of Mass applies to the principles abiding by the Copper Cycle. The first reaction was a redox reaction. This utilised Nitric Acid (strong oxidising reagent) to be combined with Metallic Copper, which formed heat from Nitrogen Dioxide (N2), and a dark green colouration (Cu(NO3)2) was observed. Second, was a double displacement where Sodium Hydroxide (NaOH) and Copper (II) Nitrate reacted together to produce blue solution.
The other two had films on the white light, green film and red film. We used the thylakoids of spinach and they were blended with 0.5 M of sucrose. In three different test tubes, we poured 150L of thylakoids mixture and then we poured them into cuvettes. We also used a spectrophotometer to measure the transmittance reading. As soon as we placed the cuvette into the spectrophotometer, we took the reading and placed it in front of the light source for 30 seconds.
2. In another experiment we could use the SDS page method, this technique uses protein separation by using a detergent to isolate particular proteins. Once this is done then the particles that are moving to the nucleus can be identified. 3. Another technique that can be used is coating gold particles with a nuclear localization signal.
Introduction Circular dichroism (CD) is form of light absorption spectroscopy that measures the difference in absorbance of right- and left-circularly polarized light (rather than the commonly used absorbance of isotropic light) by a substance. It is applicable for molecules have one or more chiral chromophores [1]. Circular dichroism = ΔA(λ) = A(λ)LCPL - A(λ)RCPL, where λ is the wavelength This technique measured a molecule over a range of wavelengths. All chiral molecules can be studied, particularly in study of large biological molecules.
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