25 mL of FD&C Blue #1, FD&C Red #40, and FD&C Yellow #5 were each obtained, and then individually placed in a 100 mL erlenmeyer flask up to the 25 mL mark. Then, a UV VIS spectrometer was connected to a laptop with the program Logger Pro. It was then calibrated by filling a cuvette to the three-quarter mark with deionized water, and running it through the calibration tool present in the program. After the spectrometer was calibrated, 5 mL of yellow dye was placed in another cuvette from the erlenmeyer flask using a 5 mL transfer pipette. The absorption level of each wavelength of the yellow dye was then recorded using Logger Pro, along with the peak absorption and its corresponding wavelength. Afterwards, the dye was discarded and the cuvette …show more content…
The volume of each dye in 1 liter was then obtained by dividing the absorption rate to be used in the replicate with the the constant found in the calibration curve equation. This value, multiplied with the amount of moles of the dyes found in 1 liter of the green Powerade, was used to calculate the exact volume of each dye present in 1 liter of the Powerade. Then, after reducing these values to fit into 100 mL of green Powerade (divided volumes by 10), and assuming the remaining volume was made up of deionized volume, it was determined that there was 35.82 mL of yellow dye, 32.27 mL of blue dye, and 31.92 mL of water present in 100 mL of green Powerade. These solutions and their respective volumes where each measured out in a 100 mL graduated cylinder, and then placed in a 100 mL erlenmeyer flask and stirred until equilibrium was reached, to create the replicate. Then, 5 mL of this solution was transferred to a cuvette using a transfer pipette, which was then placed in the UV VIS spectrometer. The absorption peaks were then recorded, and compared to the theoretical values (the original green Powerade) by determining the percent error.# Overall, it was found that the yellow dye had a percent error of 6.76% and that the blue dye had a percent error of