Green No. 3 Dye Lab Report

After analysis, we have determined Red No.3 dye is the most polar of the three dyes. This is due to the fact that this dye consistently had the lowest Rfvalues. Conversely, the most nonpolar of the solutes was Green No.3 as it had the greatest Rfvalues. This conclusion coincides with the theory that the most polar solute will have the lowest Rf values due to its attraction between the polar cellulose fibers. Further, the Green No.3 dye is the most nonpolar due to its high Rfvalues which were the result of its lack of attraction to the polar cellulose fibers. Overall, the objective of identifying the most polar and nonpolar solutes through the use of paper chromatography was attained. Through the course of this lab, we have assimilate into learning how paper chromatography can help us

This can be of greater use when using this form of paper chromatography to separate mixtures, as we have grasped an understanding as to how this form of chromatography allows for us to identify the polarity of each constituent substance. Thus, during the examination of mixtures, we will be able to identify each component based on factors such as its Rfvalue and polarity. Potential errors in this lab could have occurred in many facets. One of our procedural errors involve our failure to mark the distance traveled by the solvent in the solutions which were of 0.02M. This failure inhibited our ability to compare the Rf factors and differences in the movement of the solute in the differing concentrations. Ergo, not allowing us to identify the effect that higher concentrations of the solvent have on the solvent during paper chromatography. This error could have been fixed in the case that we would have marked the final distance of the solvent. Furthermore, after making the solvents at 0.02 M, we failed to immediately place a stopper in the flask. This may have affected the amount traveled by