ipl-logo

Liquid Chromatography Lab Report

1683 Words7 Pages
bulb-icon

Recommended: Chromatography lab report introduction

Name :SiphesihleGamede Student number : 213571124 Experiment : #10 Date : 4 March 2015 Experiment Name : High Performance Liquid Chromatography (HPLC), Quantification of caffeine in various drinks Aim The aim of this experiment was to ascertain the concentration levels of caffeine in cola soft drink samples such as coke, monster and red bull by utilising the internal standard method using HPLC. The experiment was also aimed at investigating the use of solid phase extraction for sample clean-up. Figure 1.1 shows a skematic diagram of an HPLC system. Figure 1.2 shows a skematic diagram of a solid phase extraction system. Introductory Questions Define SPE and explain the role of each of the steps used to prepare the SPE cartridge for the isolation of the analyte. Solid phase extraction (SPE) is an extraction method that uses a liquid and solid phase to isolate a single analyte or a specific type of analyte from a solution. It is usually used to clean up a sample before using a chromatographic or other analytical method to quantify the amount of analyte(s) in the sample. …show more content…

One of the reasons why SPE is a suitable technique for this analysis is the fact that there should be higher recoveries (% recoveries)since both caffeine and theophylline are polar compounds, with theophylline being more polar than caffeine which tells us that caffeine will have the longer retention time than theophylline. Other reasons include advantages such as SPE provides cleaner extracts and is easier to automate compared to other extraction …show more content…

There is a mathematical treatment called the ‘Fourier transformation’ and it is used to produce a spectrum and to interpret the data, hence the name FTIR spectroscopy. In general, infrared spectroscopy measures the ability of certain molecules to absorb infrared radiation. The radiation beam passes through a sample and then through a detector where the signal being produced is a function of optical path difference called an interferogram. The design of the FTIR is in such a way as to allow the use of capillary columns which produce sharper

Show More
Related
563 Words | 3 Pages

This addition aids in controlling the reproducibility and retention. Separation of the mixture via RP-HPLC can be done using continuous gradient or stepwise to move out the sample components. For every separation, the ideal gradient and volume must be

Read More
728 Words | 3 Pages

The lab started off by measuring critical materials for the lab: the mass of an an empty 100 mL beaker, mass of beaker and copper chloride together(52.30 g), and the mass of three iron nails(2.73 g). The goal of this experiment is to determine the number of moles of copper and iron that would be produced in the reaction of iron and copper(II) chloride, the ratio of moles of iron to moles of copper, and the percent yield of copper produced. 2.00 grams of copper(II) chloride was added in the beaker to mix with 15 mL of distilled water. Then, three dry nails are placed in the copper(II) chloride solution for approximately 25 minutes. The three nails have to be scraped clean by sandpaper to make the surface of the nail shiny; if the nails are not clean, then some unknown substances might accidentally mix into the reaction and cause variations of the result.

Read More
313 Words | 2 Pages

Elijah Brycth B. Jarlos IX-Argon 1. Multicellularity is a condition of an organism to have multicellular cells. An example of a organism who has multicellular cells are plants, animals, and humans. The main reason of why scientists have a hard time finding a good set of existing organisms to compare. Is neither the first set of organisms which is being compared is dying as fast as the second specimen is being examined or they just can’t find the right species.

Read More
615 Words | 3 Pages

The objective of the sludge lab was to determine how many different pure substances were in the sludge by using the methods and techniques we have learned throughout the year. We had to pick separation methods so we could separate our sludge and then test characteristic properties on our separated liquids and solids. This experiment made us use our knowledge on characteristic properties to pick the ones we should test to help us identify our pure substances. Characteristic properties are properties that help identify a solid or liquid. Each solid or liquid has a certain density, boiling point, solubility, flammability, so if you know what each one is then you can use that information to help you identify your solid or liquid.

Read More
412 Words | 2 Pages

Thin layer chromatography is performed by using a thin, uniform layer of silica gel and is commonly used to compare and separate most poisons, inks, and dyes. Many inks contain a colorful compound, most can be separated using TLC. The silica gel is the stationary phase. The stationary phase TLC often contains a substance which shines in UV. The mobile phase is an appropriate liquid solvent or combination of solvents.

Read More
1920 Words | 8 Pages

With respect to Infrared spectroscopy, FTIR exposes a sample to different wavelengths and measures which wavelengths are absorbed. It overall has made the structural analysis of molecules much easier by dividing bands into key components one can analyze to hypothesize functional groups present in a molecule. Attenuated total reflectance, or the ATR enables solid or liquid samples to be examined with minimal preparation by collecting a series of beams. RSC best defines FTIR-ATR

Read More
876 Words | 4 Pages

CLAIRE MUNTING 29/01/2018 Criterion C EFFECTS OF SURFACE AREA OF CALCIUM CARBONATE UPON RATE OF REACTION Calcium Carbonate Chips 1 Introduction: Within the current investigation, the effects of the surface area of Calcium Carbonate (CaCO3) in combination with Hydrochloric acid (HCl) upon its rate of reaction. CaCO3, commonly referred to as limestone, is an organic substance and is, in a sense, the crystallised “carbonic salt” of the element, calcium2. In addition to being a salt, the pH level of Calcium Carbonate is 9.91, and it is therefore, a basic substance, due to the fact that it is comprised of a pH level higher than 7, which is neutral3. HCl, however, is the bodily acid found in the stomach of human beings.

Read More
1629 Words | 7 Pages

We are going to determine the concentration of caffeine in Pepsi using gas chromatography/mass spectrometry

Read More
854 Words | 4 Pages

Experiment #1 Isolation of Caffeine from Tea Date: 11.09.14 Prepared by: Alibek Abilev Purpose The aim of the experiment was to isolate crude caffeine from tea leaves by using liquid – liquid extraction with methylene chloride, purify the crude substance by performing sublimation and determine the melting point of both crude and pure caffeine. Safety Lab coat, goggles, gloves. Methylene chloride is a carcinogenic substance, therefore should be kept in a well-ventilated place.

Read More
1696 Words | 7 Pages

Experiment #7: Column Chromatography of Food Dye Arianne Jan D. Tuozo Mr. Carlos Edward B. Santos October 12, 2015 Abstract Column chromatography is the separation of mixture’s components through a column. Before proceeding with the column chromatography itself, a proper solvent system must be chosen among the different solvents. The green colored food dye is the mixture whose components are separated.

Read More
1418 Words | 6 Pages

Caffeine is a chemical found naturally in few plants all over the world. From these plants it is processed into its pure form and then put into other things such as soda, energy drinks, tea, and pills. Energy drinks and soda are some of the most widely marketed products in the world, even though they contain the highest concentration of caffeine outside of pure caffeine supplements. It is a stimulant that affects everyone in strange but semi predictable ways. Research has been done on this topic since the 1920’s and many different conclusions have been reached.

Read More
1197 Words | 5 Pages

Range: The data generated in precision and linearity was considered for establishment of Range. The sample weight of 1250 mg to 3750 mg was taken for analysis and it was found to be within the linearity and precise. Robustness: Reliability of the method was done about deliberate variations in method parameters like a. Variation in the flow rate of mobile phase (±0.1 ml/min) b. Variation in cooler temperature (±2 nm)

Read More
1402 Words | 6 Pages

Introduction: High Performance Liquid Chromatography or also known as High Performance Liquid Chromatography is one of the most powerful and most commonly used analytical separation technique. HPLC is a form of liquid chromatography that separated solutes/compounds dissolved in the solution (High-performance liquid chromatography, 2012). It is an improved form of column chromatography, where the solvent is passed through under high pressure instead of letting it drip down due to gravity. The sample is injected into the column to separate the sample of interest. The two different phases in HPLC are mobile and stationary phase.

Read More
1076 Words | 5 Pages

It has been used to make significant contributions in many areas of physics and chemistry as well as in other areas of science. FTIR (also known as mid-IR) is the most commonly used vibrational technique for identification of material and authentication[]. Its applications include identification of functional groups or compound identification, determination of the strength of chemical bond and the calculation of thermodynamical properties. If one can obtain all the vibrational information from the molecule, its molecular structure can be determined. In the field of spectroscopy, two major techniques are used to detect molecular vibrational motions:

Read More
967 Words | 4 Pages

The internal standard method allows a very accurate analysis to be performed, since the behaviour of the species of interest is compared to that of a known substance which is present in a specified amount. It is usual to include an identical volume or mass of the internal standard into each prepared standard. This facilitates easier calculations of the composition of the

Read More

More about Liquid Chromatography Lab Report

Open Document