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Spectronic 20 Lab Report

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Discussion Week one- During the first week of this experiment we used a Spectronic 20 to measure the light absorbance and the percent of light transmitted. A Spectronic 20 is a machine that measures the amount of light that passes through the solution (Lewiston, 2014). For week one the goal of the experiment was to find the analytical for KMnO4. To work the Spectronic 20, it first must be turned on and allowed time to warm up. On the menu screen we selected to record percent of light transmitted to be displayed. Also before each time the percent transmitted was recorded, the Spectronic 20 was blanked with water. A secondary goal of week one is to compare the differences of the light absorbance and the percent of light transmitted of red, blue, and yellow. Figure 1 has the graph of the absorbance of red, blue, yellow, and KMnO4. Figure 2 has the graph of percent of light transmitted of red, blue, yellow, and KMnO4. We were able to calculate the amount of light absorbed from the percent of light transmitted. The analytical of KMnO4 is 540 nm, the analytical wavelength for the color red is 530 …show more content…

The bubbles in the carbonation would cause an inaccurate reading because the light would pass through the clear gas bubble. So do get rid of the bubbles we let the cola simmer on a hot plate. It is important to not let the cola boil because if the water in the cola evaporated it would make the cola more concentrated. The reason that the absorbance of PO43- ion and concentration graph was used instead of the transmittance of PO43- and concentration is used is because concentration and absorbance are proportional, therefore having a linear line of best fit were the absorbance could be used to calculate the PO43- concentration. Whereas if the transmittance of PO43- and concentration does not have a linear relationship so calculating the PO43- would be more

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