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Protein Integration Test Lab Report

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4. In order to determine if the nuclear localization signal will cause the cytosolic protein and any other proteins to move into the nucleus, there are experiments that can demonstrate this.

1. One such experiment includes the use of green fluorescent proteins. By fluorescently tagging the proteins, we can observe the proteins that are traveling to the nucleus.

2. In another experiment we could use the SDS page method, this technique uses protein separation by using a detergent to isolate particular proteins. Once this is done then the particles that are moving to the nucleus can be identified.

3. Another technique that can be used is coating gold particles with a nuclear localization signal. When these particles are coated, they will
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