Dr. Colleen Winters – BIO 655 Vishall G. Kaistha TITLE: “Recombination-Directed DNA Repair Promote Homologous Stimulating Transcription of Genes That That Preserves Genomic Integrity by MEN1 Is a Melanoma Tumor Suppressor”.
Proteins are an essential part of all living organisms. Proteins are macromolecules made up of amino acid chains. These chains of amino acids are held together by peptide bonds to from polypeptide chains. Each proteins function is determined by its own unique three dimensional shape and active site. Proteins have multiple functions that are important to all cells.
After the results from the three experiments, it has been concluded that Vial B contains the living organisms. This is because in each of the experiments, a characteristic of life was tested. Vial B tested positive and proved that it contained the living organisms.
In the BIO 14 Lab, we to designed a competition experiment that would tell us something about the relative fitness of the E939 and E938 strains of E. Coli and that looked into answering whether it was better to have a low or high mutation rate and under what conditions. In our experiment, we looked into what concentration can the E938 strain coexist with the E939 strain in an equal number of colonies. We plated various concentration of the strains (Figure 1) and predicted that the disparity between the growth of each strain will be greater as the concentration of the high mutator increases. Our results did not support this hypothesis, instead it showed that the E938 dominated competition at all concentrations that could be analyzed (Plates D, E & G). It could have been that we needed to dilute E938 even further than 103 cell/mL to see equal competition, but with the given results only the conclusion that can be made is that the high mutator will outcompete
In the BIO 14 Lab, we attempted to design an experiment that would tell us something about the on the phenotypic plasticity of the stimulus-response Mimosa pudica. The specific question being asked in our experiment is: What is the effect of extreme light conditions in Mimosa plants grown in both short-day and long-day conditions on the time it takes for the plants to reopen their leaves after a heavy (1g) or a light stimulus (0.3g)? Does this behavior change when under conditions of normal long-days (14 hours light/10 hours dark) or normal short-days (10 hours light/14 hours dark) versus under conditions of extreme long-day (18 hours light/6 hours dark) or extreme short-day (6 hours light/18 hours dark)? Since phenotypic plasticity is the
The system was run with the power filter, then the fish were introduced to the system. The plants were then installed after two days. The BioMax insert was removed because the plant would replace it. The plant was held up by a sponge insert sitting on top of the activated carbon. The fish were fed and the plants were observed daily.
To do this we prepared cuvettes with various different pH solutions. We used the assay buffer from experiment 1 as our positive control. This gave us a baseline because we already knew that at pH 7 we would likely see a high amount of SDH activity. Our various pH's we tested were 5, 5.5, 6, 6.5, positive control pH 7, 7.5, and 8. We mixed these with equal amounts of DCIP indicator, sodium azide, succinate, and cell fraction.
The Ubx protein was successfully cross-linked with DMP and the results of it are shown below. In order to show that cross-linking the Ubx protein with DMP successfully cross-linked, a Native Page was ran. The sample type and lane number are presented in the table below. Figure 4 Image of the finished SDS Native page on light. Lane 1 contains the Sprectra Multicolor broad Range Protein Ladder provided by ThermoFisher.
Taisya Gowlovech Bio 110 Dr. Lewis & Dr. Mallowa 29 April 2018 Biology 110 Reflection When I registered for Biology last semester, I really did not think I was going to get much out of it. I thought I simply need to get through the class for SOPHIA credit and move on. I didn't really plan on learning anything useful. I was tired of having to memorize the functions of cells and similar topics that are not really applicable to my life outside the classroom..
SDS-PAGE can be used for many different uses such as; finding important proteins in a sample and figuring out how much each protein is in a sample (Experimental Biosciences). Coomassie staining is used on SDS-PAGE gels after proteins get separated by SDS-PAGE machine, in which the SDS-PAGE gels get immersed in coomassie stain and the extra amount gets rinsed off with solvent. When the staining of SDS PAGE is completed is should show blue bands on the clear background of the SDS PAGE gel (BIO RAD). Western blotting is a lab procedure that is used to find specific proteins in a mixture
It was originally hypothesised that when mince combined with protease solution is heated in water, the rate of degradation would increase continuously from room temperature up to 50 degrees. It was expected that rate of activity would reach its optimum activity, at 50 degrees celsius and be most active between 50 and 60 degrees. Once reaching over 60 degrees, the protease would begin to denature and therefore, decrease the rate of reaction dramatically. The results of this experiment supported the hypothesis.
The aims of this practical are to examine the effects of various substances on the activity of glycogen phosphorylase by the principles of allosteric control of the enzyme and reversible phosphorylation. These principles aim to reverse the effect glycogen phosphorylase has on the conversion of glycogen to glucose-1-phosphate, i.e. causing glycogen and glucose-1-phosphate to bind and release a phosphate. The amount of phosphate formed in this experiment is measured by the principles of a spectrometry reading at 660nm. [https://www.ncbi.nlm.nih.gov/books/NBK22354/]] Introduction: Glycogen is used in animals as a form of glucose that can be kept in storage in cells until there is a diminished amount of glucose in the body, which then glycogen
Proposed Work: The research that I am involved in is exploring the role and structure of the protein Perilipin 5, which is involved in lipolytic activity in oxidative muscles. The goal of my research is to uncover the N-terminal structure of the protein Perilipin 5. The value behind knowing the structure of a protein is great. Once the structure of a protein is fully comprehended, we can more fully grasp the impact and interactions that it has on other molecules in cells and, therefore, how the cell uses this protein in lipolytic activity.
Throughout two days, these boards will hang in these selected areas and the particles will stick to the
Biochemical tests are the tests used for the identification of bacterial species based on the differences in the biochemical activities of different bacteria. Bacterial physiology differs from one species to the other. These differences in carbohydrate metabolism, protein metabolism, fat metabolism, production of certain enzymes and ability to utilize a particular compound help them to be identified by the biochemical tests. Gram’s stain was originally devised by histologist Hans Christian Gram in 1884. Gram-positive bacteria stain purple, while Gram-negative bacteria stain pink when subjected to Gram staining.
