ipl-logo

Mitochondria Lab Report

1768 Words8 Pages

Experiment 1: Questions and Hypothesis In this experiment, we were tasked to measure the amount of mitochondria in various cell fractions by looking for a biochemical marker within mitochondria called succinate dehydrogenase or SDH. This is an enzyme that catalyzes the conversion of succinate and FAD into fumarate and FADH2. The reduced electron carrier, FADH2, then carries the newly acquired electrons that it received in the Citric Acid Cycle and takes them to Ubiquinone in the mitochondrial electron transport chain (Leicht and McAllister, 2017). Measuring for the presence of this SDH can be tough so we have to get help from an artificial electron acceptor called DCIP. This acceptor is a blue color in its oxidized state, but upon the presence of SDH activity, the DCIP gets reduced and goes from blue to clear. This signals the transfer of electrons from SDHíFADH2 is present and thus means mitochondria must be present as well. However, in order for the electrons to go to DCIP we had to redirect the electrons from getting to …show more content…

To do this we prepared cuvettes with various different pH solutions. We used the assay buffer from experiment 1 as our positive control. This gave us a baseline because we already knew that at pH 7 we would likely see a high amount of SDH activity. Our various pH's we tested were 5, 5.5, 6, 6.5, positive control pH 7, 7.5, and 8. We mixed these with equal amounts of DCIP indicator, sodium azide, succinate, and cell fraction. The cell fraction that we used was P3 for all of them because we found this to have the highest SDH activity in experiment 1. We then put the same amount of P3 cell fraction in the solutions one at a time and as soon as we did this we measured the absorbance with the spec 20. We repeated this for each pH three different times. The intervals of these reading were 7 minutes apart each

Show More
Related
1100 Words | 5 Pages

After the 15 minutes, each pair was removed from their assigned temperature and mixed with its partner. The mixed solution was then poured into the appropriate tube and placed in the spectrophotometer for 120 seconds. As peroxidase was broken down a brown color appears and is measured by the spectrophotometer. The absorbance readings were recorded every 20

Read More
1488 Words | 6 Pages

Compare the result to the chart on the back of the urinary pH test strips bottle, and record data. Clean the stirring rod with water before moving on to the next test tube. Repeat this process for each increment (2 mL, 3mL, 4mL) Figure #1: Picture of bean solution mixed Figure #2: Picture of materials needed for the with alpha galactosidase experiment Safety considerations: Be careful with the beakers, glass stirring rod, and test tubes, as they could break easily and can cause cuts in the skin. DCP: A scatter plot will be used to display how the amount of alpha galactosidase (measured in mL) in the bean solution affects the glucose concentration (measured in mg/dL) and error bars to show the standard deviation.

Read More
737 Words | 3 Pages

During this experiment, mitochondria were isolated from 20.2 grams of cauliflower using extraction buffer, filtration through Miracloth, and centrifusion. Twelve samples containing various volumes of mitochondrial suspension, assay buffer, DCIP, sodium azide, and citric acid cycle intermediates were prepared to be read by a spectrophotometer. The inclusion of the dye DCIP allowed for the absorbance of the reactions between the mitochondrial suspension and the TCA cycle intermediates succinate, malonate, and oxalate to be measured, as DCIP turns from blue to colorless as the activity of succinate dehydrogenase increases. Experimental Findings Increasing the number of mitochondria in the reaction did increase the reduction of DCIP relative to the amount of mitochondrial suspension present.

Read More
1580 Words | 7 Pages

Nichole Medeisis Section A23: David Cooper 2 March 2017 Team Members: Willow Schanz, Collyn Richards, Justin Peterson Question and Hypothesis Experiment 1: Question: Which one of the cell fractions of cauliflower had the largest concentration of mitochondria, identified by the enzymatic activity of SDH? Hypothesis: Our group predicted that Pellet 2 would contain the most mitochondria. We came to this conclusion because we know that the biggest and heaviest organelles are pushed down into the first pellet because of the slow spin of the centrifuge, leaving behind anything smaller. Additionally, we know that mitochondria may not be observed in big amounts in Pellet 3 because it is too large.

Read More
1249 Words | 5 Pages

Prepare the standard solution of FeSCN2+ pipetting 18 mL of 0.200 M Fe(NO3)3 in a 20 * 150 mm test tube labeled five. Pipet 2 mL 0.0020 M KSCN into the test tube and stir. 6. Prepare a blank. Fill the plastic cuvette ¾ full with distilled H2O. Properly clean the cuvette and handle only by the top ribbed edge while wearing gloves.

Read More
1258 Words | 6 Pages

Procedure Activity 1: Fill 6 large beaker halfway with distilled water, making sure all beakers have equal amounts of water. Cut 6 30 cm dialysis bags and label each bag with a letter, A through F. Fill each dialysis bag with 15 mL of solution A through F that corresponds with the lab on each bag. For example, bag A is filled with solution A. Measure the mass of each dialysis bag and record masses of each bag in BILL. Cover the beakers with paper towel and leave the bags in the beakers overnight. Remove the dialysis bags from the beakers and let dry.

Read More
1860 Words | 8 Pages

The purpose of the lab was to measure the reaction rate of succinate to fumarate, in the isolated mitochondria of cauliflower cells. This reaction is not visible to the naked eye, and thus an artificial electron acceptor DCIP was

Read More
1065 Words | 5 Pages

Variations of the Photosynthetic Rate of Chloroplasts Extracted from Spinach Leaves Under Different Conditions is Displayed through the Hill Reaction Carla Merveille, Sana Jabeen, Lauren Jones, Juan Martell, and Bera Koskulu March 22, 2018 Abstract Chloroplasts from spinach leaves were extracted and made into a suspension in order to study the changes occurring in the electron transport chain of Photosynthesis under differing conditions. To do so, their photosynthetic rate was calculated via the changes in absorbance measured by spectrophotometry during the Hill reaction. The results show that herbicide, non-exposure to light, and protein denaturation impact the electron chain reaction, stopping the Hill reaction altogether.

Read More
759 Words | 4 Pages

Discussion The lab allowed the experimenters to learn more about the macromolecules in cells. We tested for the presence of proteins, sugars, fats, and starches in solutions. The question for this lab is “How does an experimenter detect the presence of certain macromolecules in a substance?” We found the answers throughout the lab.

Read More
449 Words | 2 Pages

In life, photosynthesis and cellular respiration are two processes that allow life to survive. It is a cycle that uses each others reactants and products to fuel each other. The release of carbon dioxide and oxygen through photosynthesis or cellular respiration help the atmosphere stay stable. All cells in plants and animals perform cellular respiration to maintain homeostasis and grow. Cellular respiration harvests energy in glucose to produce ATP, which powers the cell.

Read More
172 Words | 1 Pages

Given the Citric Acid Cycle or Kreb's Cycle or Tricarboxylic acid, an overall decrease in the ATP production in mitochondria can be caused by deficiency of succinyl coenzyme A synthethase).This enzyme act on the conversion of of the succinyl group to succinate. Decrease in ATP levels can be seen in the substrate-level phosphorylation of converting succinyl CoA to succinate. No, the entire cycle will not be functional resulting to acidosis. The cells would choose to produce lactic acid as means of obtaining ATP.

Read More
2102 Words | 9 Pages

Abstract The purpose of this experiment is to test for mitochondrial activity by isolating different organelles using the differential centrifugation process. Studying mitochondria is extremely important because they control the death and life of the cell by regulating the apoptotic signals (Frezza et al 2007). Also they are responsible for the metabolic reactions (aerobic respiration) and the production of ATP (Frezza et al 2007). Three hypotheses were formed based on my knowledge.

Read More
862 Words | 4 Pages

Mitosis is the process and development in which new cells divide, through cell division, consisting of four different stages; Prophase, Metaphase, Anaphase and Telophase. The concept of mitosis is to create new cells, by duplicating the contents of a parent cell nucleus into two new daughter cell nuclei. The cells are contain an identical number of chromosomes and genetic content of the original parent cell. http://www.biology-online.org/dictionary/Mitosis. How exactly new cells are produced, is by DNA Replication, which is passed on to the steps of mitosis.

Read More
1407 Words | 6 Pages

Eukaryotic cells divide and reproduce in two ways: mitosis and meiosis. Mitosis is a process of nuclear division that chromosomes are separated into two identical sets of chromosomes. The purpose of mitosis is cell regeneration, growth, and asexual reproduction. Meiosis, on the other hand, is a special type of cell division which reduces the chromosome number by half. To achieve halving the genome, DNA replication is followed by two consecutive rounds of nuclear division during meiosis.

Read More
638 Words | 3 Pages

Before I can go into detail about the three steps of mitosis that are presented in the question I must first summarize mitosis. Mitosis is actually a process of nuclear division that is nicely broken up into five stages. The stages are prophase, prometaphase, metaphase, anaphase, and telophase. Mitosis conserves the chromosome number by assigning replicated chromosomes equally to each of the daughter nuclei. Mitosis takes place in eukaryotic cells (Campbell, pg. G-22).

Read More

More about Mitochondria Lab Report

Open Document